Cymbopogon spp. ve Helianthemum spp. Bitki Ekstraktlarının In-Vitro Antibakteriyal Aktivitelerinin Araştırılması
Patojen mikroorganizmalara karşı kullanılan antibakteriyel maddelere direncin artması ve gıda katkı maddelerinin istenmeyen yan etkileri nedeniyle, son yıllarda yeni bitki kaynaklı antibakteriyel madde arayışını arttırmıştır. Bu çalışmada, Türkiye’den toplanan Cymbopogon jwarancusa, Hindistan’dan toplanan Cymbopogon citratus ve Güney Afrika’dan toplanan Cymbopogon nardus türleri ile Mısır’dan toplanan Helianthemum kahiricum ve Suriye’den toplanan Helianthemum lippi türü bitki ekstraktlarının hastane enfeksiyonlarının etiyolojisinde yer alan Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aureginosa patojen türleri ile enteropatojenlerden; Salmonella typhi, Escherichia coli, türlerinin antibakteriyel aktivitelerinin disk difüzyon yöntemiyle araştırılması amaçlandı. Disk diffüzyon sonuçlarına göre, yalnızca E. coli ve S. aureus’a karşı tüm bitki ekstraktlarının (20μg/disk) farklı düzeylerde zon çapı oluşturduğu (16-38 mm) belirlendi. Bitki türleri içerisinde en yüksek antibakteriyel aktivite C. citratus ekstraktında tespit edildi. Sonuç olarak, başta C. citratus türü ekstraktı olmak üzere, araştırılan bitki ekstraktlarının sağlık, farmasötik, kozmetik ve gıda endüstrisi gibi birçok alanda mikroorganizmalara karşı etkili olduğu ve bu konuda daha ileri düzeyde in vitro ve in vivo çalışmaların yapılması gerektiği düşünülmektedir.
Anahtar Kelimeler:
Disk difüzyon, Antibakteriyel aktivite, Hastane enfeksiyonu
Investigation of Antibacterial Activities of Plant Extracts Prapered from Cymbopogon ssp. and Helianthemum ssp.
New plant-derived antibacterial agent researches have increased in recent years, because of increased resistance to antimicrobial agents that used against to pathogen microorganisms, and unwanted side effects of food additives. The aim of this research was to investigate the, in-vitro antibacterial activities and collected from Turkey Cymbopogon jwarancusa on, collected from India Cymbopogon citratus and collected from Egypt with Cymbopogon nardus species collected from South Africa Helianthemum kahiric and collected from Syria Helianthemum lippi species of plants extracts on as with pathogens species; Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aureginosa and as from entheropathogens Salmonella typhi, Escherichia coli, of which also in the etiology of nosocomial infections, by disk diffusion method.The diameter of the inhibition zone in the disc-diffusion method shows that all the plant extracts (20μg/disc) have antibacterial activity only on E.coli and S.aureus different levels (16-38 mm). C. citratus extract showed the highest antibacterial activity with disc diffusion method. In conclusion, particularly C.citratus plant extract, besides other plant extracts are thought to be effective against to microorganisms in many areas like medical, cosmetic and food industries. But there is need new in vitro and in vivo studies in future.
Keywords:
Antibacterial activity, Disc diffusion, Nosocomial,
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- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 2018
- Yayıncı: Çankırı Karatekin Üniversitesi
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