Pınar COŞKUN, Sueda TÜRK, Mehmet Ali KISAÇAM, Filiz KAZAK

Rutin Ratlarda Metotreksata Bağlı Hepatik Oksidatif Stresi Hafifletir

Bu çalışma, rutinin ratlarda metotreksata bağlı hepatik oksidatif stres üzerindeki potansiyel antioksidan etkilerini değerlendirmeyi amaçlamıştır. Wistar Albino ratlar (n=24) dört gruba ayrıldı: Kontrol grubu (K); metotreksat (M, 20 mg/kg, tek doz) grubu; rutin (R, 50 mg/kg/gün, 15 gün) grubu; ve metotreksat + rutin [MR, metotreksat (20 mg/kg, tek doz) + rutin (50 mg/kg/gün, 15 gün)] grubu. Ratlar onaltıncı gün sakrifiye edildi. Karaciğer dokuları alındı. Malondialdehit (MDA), vitamin C, total glutatyon (tGSH), nikotinamid adenin dinükleotid fosfat (NADPH) seviyeleri ve glutatyon peroksidaz (GPx) ve glukoz-6-fosfat dehidrojenaz (G6PD) aktiviteleri kolorimetrik olarak belirlendi. M grubunda diğer gruplara göre MDA düzeyleri artmış ve C vitamini düzeyleri azalmıştır (P

Rutin Attenuates Methotrexate-Induced Hepatic Oxidative Stress in Rats

This study aimed to assess the antioxidant effects of rutin on methotrexate-induced hepatic oxidative stress. Wistar Albino rats (n=24) were separated into 4 groups: control group (C); methotrexate (M, 20 mg/kg, single dose) group; rutin (R, 50 mg/kg/day, for fifteen days) group; and methotrexate + rutin [MR, methotrexate (20 mg/kg, single dose) + rutin (50 mg/kg/day, for fifteen days)] group. Rats were sacrificed on the sixteenth day and liver tissues were removed. The liver glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), malondialdehyde (MDA), nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), and vitamin C were determined colorimetrically. Malondialdehyde increased and vitamin C decreased in the M group compared to the others (P

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