Harun ALBAYRAK, Emre OZAN, Selma KAYA, Mitat KURT, Hamza KADİ, Abdullah CAVUNT, Cenk BOLUKBASİ, Zafer PEKMEZCİ

Investigation of tick- and mosquito-borne flaviviruses in Blacksea Region

Flavivirus cinsi içinde yer alan türler dünya üzerinde halk sağlığı problemleri oluşturmaktadır. Asyada artan Japonensefalitis ve dang virus humması vakaları, Güney Amerika ve Afrikada sık görülen sarı humma salgınları ve Amerikadabaştan başa yayılan batı nil virusu, flavivirusların coğrafi yoğunluğunu göstermektedir. Bu çalışmada, Karadeniz bölgesindeçeşitli memeli hayvan türlerinden (sığır, koyun, keçi ve manda) toplanan 2340 olgun kene (675 kene havuzu) ve Culex spp., Anopheles spp., Aedes spp., ve Culicoides spp.den oluşan 3226 sinek (142 sinek havuzu) sinek ve kenelerle taşınanflavivirusların varlığı yönünden reverz-transkriptaz zincir reaksiyonu metodu ile test edildi. Örneklerin hiçbirisinde flavivirus genomik RNAsı tespit edilemedi. Bu çalışma, hem kene hem de sineklerle taşınan flavivirusların varlığı yönünden Türkiyedeyapılan ilk çalışmadır.

Karadeniz Bölgesinde kene ve sineklerle taşınan flavivirusların araştırılması

Species within the Flavivirus genus pose public health problems around the world. Increasing cases of Dengue and Japanese encephalistis virus in Asia, frequent outbreaks of Yellow fever virus in Africa and South America, and the ongoing spread of West Nile virus throughout the America, show the geographical burden of flavivirus diseases. In this study, a total of 2340 adult ticks (675 hard tick pools) collected from the variety of mammalian species (cattle, sheep, goat and buffalo) and 3226 mosquitoes (142 mosquito pools) including Culex spp., Anopheles spp., Aedes spp., and Culicoides spp., trapped in Blacksea region of northern Turkey were surveyed for the presence of RNA from mosquito-borne flaviviruses (MBFV) and tick-borne flaviviruses (TBFV) by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. No flavivirus genomic RNA was detected in these samples. This is the first study about both the TBFV and MBFV infections in Turkey.

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Albayrak H., Ozan E., Kurt M., 2010. Molecular detection of Crimean-Congo haemorrhagic fever virus (CCHFV) but not West Nile virus (WNV) in hard ticks from provinces in northern Turkey. Zoonoses Public Hlth., 57, e156e160.
Anonim, 2010. Update on average annual values of heat, humidity, and rainfall, http//www. dmi.gov.tr/ tahmin/ il-ve- ilceler.aspx [accessed on 8 October, 2010].
Burke DS., Monath TP., 2001. Flaviviruses. In Fields thVirology, Eds., DM. Knipe, PM. Howley, 4 edn, Lippincott Williams & Wilkins,Philadelphia.
Calisher CH., Karabatsos N., Dalrymple JM., Shope RE., Porterfield JS., Westaway EG., Brandt WE., 1989. Antigenic relationships betweenflaviviruses as determined by cross-neutralization tests with polyclonal antisera. J.Gen. Virol., 70, 3743.
Charrel RN., Zaki AM., Attoui H., Fakeeh M., Billoir F., Yousef AL., De Chesse R., De Micco P., Gould EA., De Lamballerie, X., 2001. Complete coding sequence of the Alkhurma virus, a tick-borne flavivirus causing severe hemorrhagic fever in humans in Saudi Arabia. Biochem. Bioph. Res. Co., 287, 455461.
Dik B., Yagcı S., Linton YM., 2006. A review of species diversity and distribution of Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) in Turkey. J. Nat. Hist., 40, 19471967.
Ergunay K., Saygan MB., Aydogan S., Menemenlioglu D., Turan HM., Ozkul A., Us D., 2007a. West Nile virus seroprevalance in blood donors from central Anatolia, Turkey. Vector Borne Zoonotic Dis., 7, 157161.
Ergunay K., Ozer N., Us D., Ozkul A., Simsek F., Kaynas S., Ustacelebi S., 2007b. Seroprevalance of West Nile virus and tick-borne encephalitis virus in southeastern Turkey: First evidence for tick-borne encephalitis virus infections. Vector Borne Zoonotic Dis., 10, 771775.
Gaunt MW., Sall AA., De Lamballerie X., Falconar AK., Dzhivanian TI., Gould EA., 2001.Phylogenetic relationships of flavivirusescorrelate with their epidemiology, disease association and biogeography. J. Gen. Virol.,82, 18671876.
Grard G., Moureau G., Charrel RN., Lemasson JJ., Gonzalez JP., Gallian P., Gritsun TS., Holmes EC., Gould EA., De Lamballerie X., 2007. Genetic characterization of tick-borne flaviviruses: New insight into evolution, pathogenetic determinants and taxonomy. Virology, 361, 8092.
Hubalek Z., Halouzka J., 1999. West Nile fever a reemerging mosquito-borne viral disease in Europe. Emerg. Infect. Dis., 5, 643650.
Kurt R., Meece JK., Henkel JS., Shukla SK., 2002. Birds, migration and emerging zoonoses: West Nile virus, lyme disease, influenza A and enteropathogens. Clin. Med. Res., 1, 512.
Maher-Sturgess SL., Forrester NL., Wayper PJ., Gould EA., Hall RA., Barnard RT., Gibbs MJ., 2008. Universal primers that amplify RNA from all three flavivirus subgroups. Virology J., 5, 110.
Ozkul A., Yıldırım Y., Pinar D., Akcali A., Yilmaz V., Colak D., 2006. Serological evidence of West Nile virus (WNV) in mammalian species in Turkey. Epidemiol. Infect., 134, 826829.
Pierre V., Drouet MT., Deubel V., 1994. Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction. Res. Virology., 145, 93104.
Spackman E., Senne DA., Myers TJ., Bulaga LL., Garber LP., Perdue ML., Lohman K., Daum LT., Suarez DL., 2002. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol., 40, 32563260.
Thiel HJ., Collett MS., Gould EA., Heinz FX., Meyers G., Purcell RH., Rice CM., Houghton M., 2005. Flaviviridae. In Virus taxonomyeighth report of the international committee on taxonomy of viruses, Eds., CM. Fauquet, MA. Mayo, J. Maniloff, U. Desselberger, LA. Ball, Academic Press, San Diego., 981-999
Uyar Y., Akcali A., Carhan A., Ozkaya E., Ertek M., 2007. Seroprevalance of tick-borne encephalitis virus (TBEV) among cases with tick bite history in Turkey. Turk Hij. Den. Biyol. Derg., 64, 2125.