Umut Eren ERDOĞDU, Haci Murat ÇAYCI, Ali TARDU, Ufuk ARSLAN, Yasemin BUDAK, Özcan EREL, Hakan DEMİRCİ, Hasan ÇANTAY

Helicobacter pylori ve tiyol-disülfid homeostazı arasındaki ilişki: Prospektif, gözlemsel bir çalışma

Amaç: Helicobacter pylori (HP) enfeksiyonu, hücresel düzeyde inflamasyona ve oksidatif strese neden olur. Bu çalışmada, oksidatif stresin bir göstergesi olan tiyol-disülfid homeostazı (TDH) ve HP arasındaki olası ilişkiyi değerlendirmeyi amaçladık.Yöntemler: Dispepsi yakınması ile başvuran ve gastroskopi uygulanan toplam 53 hastanın tıbbi verileri prospektif olarak değerlendirildi. Hastalar gastrik biyopsi sonucuna göre HP-pozitif (+) ve HP-negatif (-) olarak iki gruba ayrıldı. Hastaların demografik verileri, plazma ferik indirgeme kabiliyeti (FRAP), iskemi modifiye albümin (IMA), native tiyol, total tiyol, disülfid ve malondialdehit (MDA) düzeyleri kaydedildi ve iki grup arasında karşılaştırıldı.Bulgular: Doğal tiyol (451,03 mmol/L ve 407.03 mmol/L, p=0.005) ve toplam tiyol (493.20 mmol/L ve 456.40 mmol/L, p=0,027) seviyeleri HP (+) grubunda HP (-) grubuna göre anlamlı olarak daha yüksekti. Disülfid düzeyleri ve disülfid / native thiol, disülfid/total tiyol ve native tiyol / total tiyol oranları HP (+) ve HP (-) grupları arasında benzerdi. FRAP, HP (+) grubunda HP (-) grubuna göre daha düşük olmasına rağmen, bu fark istatistiksel olarak anlamlı değildi (0,94 mmol/L ve 1,10 mmol/L). IMA ve MDA düzeylerinde gruplar arasında istatistiksel olarak anlamlı bir fark saptanmadı. Sonuç: HP kaynaklı gastrit gibi oksidatif stresin arttığı koşullar altında serum tiyol-disülfid düzeylerinin yükselmesi görülebilir. HP enfeksiyonunda yüksek serum tiyol-disülfid düzeylerinin antioksidan veya pro-oksidan durumu gösterip göstermediğini incelemek için daha fazla sayıda hasta ve alt grup analizi içeren çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

Helicobacter pylori, homeostaz, tiyol, disülfid, oksidatif stres

Relationship between Helicobacter pylori and thiol-disulfide homeostasis: A prospective observational study

Aim: Helicobacter pylori (HP) infection causes inflammation and oxidative stress at a cellular level. In the present study, we aimed to evaluate the possible relationship between HP and thiol-disulfide homeostasis (TDH), a novel indicator of oxidative stress.Methods: Medical data of a total of 53 patients admitted with persistent dyspepsia and undergoing gastroscopy were evaluated prospectively. The patients were divided into two groups, based on the result of gastric biopsy, as HP-positive (+) and HP-negative (-). Demographic data, ferric-reducing ability of plasma (FRAP), ischemia-modified albumin (IMA), native thiol, total thiol, disulfide, and malondialdehyde (MDA) levels of the patients were recorded and compared between the two groups.Results: The native thiol (451.03 mmol/L vs. 407.03mmol/L, p=0.005) and total thiol (493.20 mmol/L vs. 456.40 mmol/L, p=0.027) levels were significantly higher in the HP (+) group than in the HP (-) group. The disulfide levels and disulfide/native thiol, disulfide/total thiol and native thiol/total thiol ratios were similar between the HP (+) and HP (-) groups. Although the FRAP was lower in the HP (+) group than in the HP (-) group, this difference was not statistically significant (0.94 mmol/L vs. 1.10 mmol/L). No statistically significant difference was found between the groups in the IMA and MDA levels. Conclusion: In this study, oxidative status of HP patients was evaluated in several different methods. Among them, only elevated native thiol and total thiol levels were found in HP-induced gastritis. There is a need for further studies involving a larger number of patients and a subgroup analysis to examine whether elevated serum thiol-disulfide levels in HP infection suggest an antioxidant or pro-oxidant status.

Keywords:

Helicobacter pylori, homeostasis, thiol, disulfide, oxidative stress,

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