FARKLI KOŞULLARDA BULUNAN ANTİK İSKELET ÖRNEKLERİNDEN DEKONTAMİNASYON SÜRECİ İLE İLGİLİ METODOLOJİK YAKLAŞIM
Antik kalıntılardan DNA elde edilebileceğinin anlaşılması antropologlarınçalışmalarına yeni bir boyut kazandırmıştır. Bu önemli katkıya rağmen antik DNAanalizleri, üzerinde çalışılan örneklerin doğası gereği birçok güçlüğü deberaberinde getirir. Bu çalışmalarda en önemli sorun yeterince korunmuş örnekleriseçmek ve bu örneklerden mümkün olan en kaliteli DNA’yı elde etmektir. Antikörnekler DNA eldesinden önce farklı aşamalarda maruz kaldıkları dışkontaminantlardan arındırılmalıdır. Arındırma sürecinin başarısı, alınacaksonuçların güvenirliliği açısından büyük önem taşır. Süreç sonunda alınan hersonuç araştırmacı tarafından kontaminasyon nedeni ile gerçekleşmediği gösterilereksavunulmalıdır. Antik DNA çalışmalarında kullanılan metodolojik yaklaşım bunedenle büyük önem taşır. Çalışmada Femur ve Talus kemikleri bu metodolojiyeuygunlukları açısından kıyaslanmıştır. Korunma düzeyleri farklı olan örnekler DNAeldesi öncesi hazırlık sürecinde farklı uygulamalar gerektirmiştir. Sonuç olarak ikiörnek tipinden elde edilen DNA miktarı arasında belirgin bir fark bulunamamış veörneklerin modern insan DNA’sı kontaminasyonundan arındırılabildiğigözlenmiştir.
A Methodological Approach Related to the Decontamination Process from Ancient Skeletal Samples in Different Conditions
Understanding that DNA can be obtained from ancient remains has made valuable contribution to the work of anthropologists. Despite this important contribution, ancient DNA analysis brings many challenges due to the nature of the studied specimens. The most important problem in these studies is to select well-preserved samples and to obtain the best quality DNA possible from these samples. Ancient samples should be purified from external contaminants that they are exposed to at different stages prior to DNA extraction. The success of the purification process is of great importance in terms of the reliability of the results. Results obtained at the end of the process should be defended by researcher showing that they did not take place due to contamination. The methodological approach used in ancient DNA studies is therefore of great importance. In the study, the femur and talus bones were compared in terms of their suitability for this methodology. Samples with different levels of preservation required different applications in the pre-DNA preparation stage. As a result, no significant difference was found between the amount of DNA obtained from the two sample types and it was observed that the samples were successfully purified from modern human DNA contamination.
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