Salivary cortisol and total antioxidant capacity levels of children with untreated dental caries

Salivary cortisol and total antioxidant capacity levels of children with untreated dental caries

Aim: The study aimed to evaluate the relationship between the caries frequency, which was determined using different caries indexesand biological parameters such as salivary cortisol and total antioxidant capacity (TAC).Materials and Methods: 140 patients between the ages of 5-13 without systemic disease were included in the study. Saliva sampleswere collected from patients whose DMFT (dmft) and PUFA (pufa) indexes were recorded, and these samples were centrifuged at5,000 rpm for 10 minutes. All samples were stored at -20 °C until measurement. Saliva cortisol and TAC levels were measured usingthe ELISA kit. All data were analyzed with SPSS v.22.Results: The mean DMFT/dmft and PUFA/pufa values were estimated as 6.72±4.37, 1.39±2.08, respectively. There was no significantcorrelation between caries indexes and biochemical parameters.However, there was a correlation between TAC and cortisol levelsin saliva.Conclusion: Within the limitations of this study, it was determined that high risk of caries in patients could not affect salivary cortisoland TAC levels; however, there was a positive correlation between salivary cortisol and TAC levels. Further studies are needed tobetter understand the background of the pathophysiological changes in these biochemical parameters and their relationship withcaries.

___

  • 1. Kutsch VK. Dental caries: an updated medical model of risk assessment. J Prosthet Dent 2014;111:280-5.
  • 2. Hegde AM, Rai K, Padmanabhan V. Total antioxidant capacity of saliva and its relation with early childhood caries and rampant caries. J ClinPediatr Dent 2009;33:231-4.
  • 3. Wright JT. The Burden and Management of Dental Caries in Older Children. PediatrClin North Am 2018;65:955-63.
  • 4. Fontana M, Young DA, Wolff MS. Evidence-based caries, risk assessment, and treatment. Dent Clin North Am 2009;53:149-61.
  • 5. Nelson S, Lee W, Albert JM, et.al. Early maternal psychosocial factors are predictors for adolescent caries. J Dent Res 2012;91:859-64.
  • 6. Tang C, Quinonez RB, Hallett K, et. al. Examining the association between parenting stress and the development of early childhood caries. Community Dent Oral Epidemiol 2005;33:454-60.
  • 7. Tikhonova S, Booij L, D'Souza V, et. al. Investigating the association between stress, saliva and dental caries: a scoping review. BMC Oral Health 2018;13;18:41.
  • 8. Lupien SJ, McEwen BS, Gunnar MR, et.al. Effects of stress throughout the lifespan on the brain, behaviour and cognition. Nat Rev Neurosci 2009;10:434-45.
  • 9. Caruso S, Gatto R, Cinque B, et. al. Association between salivary cortisol level and caries in early childhood. Eur J Paediatr Dent 2018;19:10-5.
  • 10. Jessop DS, Turner-Cobb JM. Measurement and meaning of salivary cortisol: a focus on health and disease in children. Stress 2008;11:1-14.
  • 11. Rai K, Hegde AM, Shetty S, et al. Estimation of salivary cortisol in children with rampant caries. J ClinPediatr Dent 2010;34:249-52.
  • 12. Pani SC. The Relationship between Salivary Total Antioxidant Capacity and Dental Caries in Children: A Meta-Analysis with Assessment of Moderators. J IntSocPrev Community Dent 2018;8:381-5.
  • 13. Boyce WT, Den Besten PK, Stamperdahl J, et. al. Social inequalities in childhood dental caries: the convergent roles of stress, bacteria and disadvantage. Soc Sci Med 2010;71:1644-52.
  • 14. Aschbacher K, O'Donovan A, Wolkowitz OM, et. al. Good stress, bad stress and oxidative stress: insights from anticipatory cortisol reactivity. Psychoneuroendocrinology 2013;38:1698-708.
  • 15. Giebułtowicz J, Wroczyński P, Samolczyk-Wanyura D. Comparison of antioxidant enzymes activity and the concentration of uric acid in the saliva of patients with oral cavity cancer, odontogenic cysts and healthy subjects. J Oral Pathol Med 2011;40:726-30.
  • 16. Vrbanović E, Lapić I, Rogić D, et al. Changes in salivary oxidative status, salivary cortisol, and clinical symptoms in female patients with temporomandibular disorders during occlusal splint therapy: a 3-month follow up. BMC Oral Health 2019 6;19:100.
  • 17. Dodwad R, Betigeri AV, Preeti BP. Estimation of total antioxidant capacity levels in saliva of caries-free and caries-active children.ContempClin Dent 2011;2:17- 20.
  • 18. Hellhammer DH1, Wüst S, Kudielka BM. Salivary cortisol as a biomarker in stress research. Psychoneuroendocrinology 2009;34:163-71.
  • 19. Rolfsjord LB, Bakkeheim E, Berents TL, et.al. Morning Salivary Cortisol in Young Children: Reference Values and the Effects of Age, Sex, and Acute Bronchiolitis. J Pediatr 2017;184:193-8.
  • 20. Pereslegina IA. The activity of antioxidant enzymes in the saliva of normal children. Lab Delo1989;20-3.
  • 21. Kamran R, Farooq W, Faisal MR, et al. Clinical consequences of untreated dental caries assessed using PUFA index and its covariates in children residing in orphanages of Pakistan. BMC Oral Health 2017 11;17:108.
  • 22. Monse B, Heinrich-Weltzien R, Benzian H, et al. PUFA- -an index of clinical consequences of untreated dental caries. Community Dent Oral Epidemiol 2010;38:77- 82.
  • 23. Kambalimath HV, Dixit UB, Thyagi PS. Salivary cortisol response to psychological stress in children with early childhood caries. Indian J Dent Res 2010;21:231-7.
  • 24. Gunnar MR, White BP. Salivary cortisol measures in infants and child assessment. In: Singer LT, Zeskind PS, editor. Bio behavioral assessment of the infants, 1st ed. New York; Guilford Publications 2001;167-89.
  • 25. Kumar D, Pandey RK, Agrawal D, et al. An estimation and evaluation of total antioxidant capacity of saliva in children with severe early childhood caries. Int J Paediatr Dent 2011;21:459-64.
  • 26. Tulunoglu O, Demirtas S, Tulunoglu I. Total antioxidant levels of saliva in children related to caries, age, and gender. Int J Paediatr Dent 2006;16:186-91.
  • 27. Banda NR, Singh G, Markam V. Evaluation of total antioxidant level of saliva in modulation of caries occurrence and progression in children. J Indian SocPedodPrev Dent 2016;34:227-32.
  • 28. Mahjoub S, Ghasempour M, Gharage A, et. al. Comparison of total antioxidant capacity in saliva of children with severe early childhood caries and cariesfree children. Caries Res 2014;48:271-5.
  • 29. Uberos J, Alarcón JA, Peñalver MA, et. al. Influence of the antioxidant content of saliva on dental caries in an at-risk community. Br Dent J 2008 26;205:5.
  • 30. AlAnazi GS, Pani SC, AlKabbaz HJ. Salivary antioxidant capacity of children with severe early childhood caries before and after complete dental rehabilitation. Arch Oral Biol 2018;95:165-9.
  • 31. Muchandi S, Walimbe H, Bijle MN, et. al. Comparative evaluation and correlation of salivary total antioxidant capacity and salivary pH in caries-free and severe early childhood caries children. J Contemp Dent Pract 2015 1;16:234-7.
  • 32. Prabhakar A, Dodawad R, Os R. Evaluation of Flow Rate, pH, Buffering Capacity, Calcium, Total Protein and Total Antioxidant Levels of Saliva in Caries Free and Caries Active Children-An In Vivo Study. Int J Clin Pediatr Dent 2009; 2:9-12.
  • 33. Vrbanović E, Alajbeg IZ, Vuletić L, et al. Salivary Oxidant/ Antioxidant Status in Chronic Temporomandibular Disorders Is Dependent on Source and Intensity of Pain - A Pilot Study. Front Physiol 2018;17;9:1405.
Annals of Medical Research-Cover
  • Yayın Aralığı: Aylık
  • Yayıncı: İnönü Üniversitesi Tıp Fakültesi
Sayıdaki Diğer Makaleler

A rare case of rhinosinusitis: Three different complications in a patient

Erdoğan OKUR, Mehmet Emre SİVRİCE, Hasan YASAN, Yusuf Çağdaş KUMBUL, Sarper SAYIN

Clinical results and complications in patients Operated for colloid cyst

Hasan Emre AYDIN, İsmail KAYA, Gönül GÜVENÇ, Ceren KIZMAZOĞLU, Orhan KALEMCİ, İnan UZUNOĞLU, Gökhan GÜRKAN, İlker Deniz CİNGÖZ, Murat SAYIN, Nurullah YÜCEER, Ali Osman MUCUOĞLU, Baran TAŞKALA

Retrospective evaluation of anesthesia and surgery methods in proximal hypospadias cases

Serpil SANCAR, Derya KARASU, Esra ÖZÇAKIR, Mete KAYA, Ümran KARACA, Şeyda Efsun ÖZGÜNAY, Şermin EMİNOĞLU

Intensive care nurses’ attitudes toward the “dying with dignity” principles and affecting factors

Hakime ASLAN, Mehmet DÜNDAR

Foreign bodies in the external auditory canal: A single center experience

Mustafa ALTINTAŞ

Assessment of upper airway obstruction in children with specific learning disorder

Ayla UZUN ÇİÇEK, Adem BORA

Comparison of influence on psychosocial factors of single and recurrent sexual abuse in childhood

Fadime YÜKSEL, Nilüfer KOÇTÜRK, Esra ÇÖP

Microorganisms isolated from external ear cultures and their antimicrobial susceptibility in patients with chronic suppurative otitis media: A six years experience

Mustafa Koray BALCI, Tuba MUDERRİS, Rahim ÖZDEMİR, Süreyya GÜL YURTSEVER, Selçuk KAYA

Candidemia in adult intensive care units: Analysis of a 4 year process in a tertiary hospital in Turkey

Leman Acun DELEN, Esra ERDOĞAN, Mehmet Mustafa ERDOĞAN

Oral mucous membrane condition of the patients with chronic obstructive pulmonary disease and the measures taken for the protection

Derya TÜLÜCE, Sevinç KUTLUTÜRKAN, Emine KAPLAN SERİN