Cuma Mertoglu, Can Turkler, Pasa Ulug, Nahit Ata

Is there a role of antisperm antibodies in women with unexplained infertility - a Turkish pilot cross sectional case control study

Aim: Although some studies indicate that the presence of antisperm antibody (ASA) is important in the etiology of female infertility, this remains a controversial issue. The aim of this study was to determine whether the presence of ASA was important or not in primary and secondary unexplained infertility patients. Materials and Methods: Female patients (n = 90) were divided into three groups as follows: the primary infertility group (n = 30), the secondary infertility group (n = 28) and a control group (n = 32). In addition to laparoscopic evaluations, the presence of ASA in the study groups was also examined in serum and peritoneal washing liquid. The ASA - immunoglobulin G was measured using the Enzyme-Linked ImmunoSorbent Assay (ELISA) method with a qualitative measurement kit. Results: When the demographic data were analyzed, it was observed that the study groups were homogeneous in terms of age and period of infertility. The results of the study demonstrated that, when compared to the secondary infertility and control groups, the ASA positivity in the serum in the primary infertility group was significant (p < 0.05). However, when the groups were compared, there was no difference in terms of ASA positivity in peritoneal washing fluid (p > 0.05). Conclusion: This study showed that the presence of ASA in serum may be an impact on the etiology of unexplained primary infertility

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1. Zegers-Hochschild F, Adamson GD, Dyer S, et al. The International Glossary on Infertility and Fertility Care, 2017. Fertil Steril 2017;108:393-406.
2. Yildirim E, Derici MK. A Case-Control Study on the Oxidative Status in Women with Polycystic Ovary Syndrome Treated with Clomiphene Citrate. Med Sci Monit 2019;25:3910-7.
3. Bobak L, Bobakova D, Vaczy Z, et al. Incidence of antibodies in women after failure of assisted reproduction. BratislLekListy 2014;115:145-9.
4. Wagner A, Holland OJ, Tong M, et al. The role of SPRASA in female fertility. ReprodSci 2015;22:452-61.
5. Bhande S, Naz RK. Molecular identities of human sperm proteins reactive with antibodies in sera of immunoinfertile women. MolReprod Dev 2007;74:332- 40.
6. Stern JE, Dixon PM, Manganiello PD, et al. Antisperm antibodies in women: variability in antibody levels in serum, mucus, and peritoneal fluid. Fertil Steril 1992;58:950-8.
7. Putowski L, Darmochwal-Kolarz D, Rolinski J, et al. The immunological profile of infertile women after repeated IVF failure (preliminary study). Eur J ObstetGynecolReprodBiol 2004;112:192-6.
8. Zhang H, Zhao E, Zhang C, et al. The Change of Semen Superoxide Dismutase and Acrosin Activity in the Sterility of Male Patients with Positive Antisperm Antibody. Cell BiochemBiophys 2015;73:451-3.
9. Heng BC, Huang W, Zhong X, et al. Roles of antiphospholipid antibodies, antithyroid antibodies and antisperm antibodies in female reproductive health. Integr Med Int 2015;2:21-31.
10. Chang TH, Jih MH, Wu TC. Relationship of sperm antibodies in women and men to human in vitro fertilization, cleavage, and pregnancy rate. Am J ReprodImmunol 1993;30:108-12.
11. Somigliana E, Paffoni A, Busnelli A, et al. Age-related infertility and unexplained infertility: an intricate clinical dilemma. Hum Reprod 2016;31:1390-6.
12. Kamieniczna M, Domagała A, Kurpisz M. The frequency of antisperm antibodies in infertile couples--a Polish pilot study. MedSciMonit 2003;9:CR142-9.
13. Wang YX, Zhu WJ, Jiang H. Sperm exposure during menses is a risk factor for developing antisperm antibody (ASA) in female. ArchGynecolObstet 2013;288:1145-8.
14. Bahraminejad R, Kadanali S, Erten O, et al. Reproductive failure and antisperm-antibody production among prostitutes. ActaObstetGynecolScand 1991;70:483-5.
15. Vujisić S, Lepej SZ, Jerković L, et al. Antisperm antibodies in semen, sera and follicular fluids of infertile patients: relation to reproductive outcome after in vitro fertilization. Am J ReprodImmunol 2005;54:13-20.
16. Esteves SC, Schneider DT, Verza S Jr. Influence of antisperm antibodies in the semen on intracytoplasmic sperm injection outcome. IntBraz J Urol 2007;33:795- 802.