Gülçin AKÇA, İlke Doğa ŞEKER, Tayfun ALAÇAM

In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens

Aim: To investigate the antimicrobial eects of Citrus limonum and calcium hydroxide comparatively and to evaluate MIC, MBC, and MFC levels for some microorganismal strains of the root canal microbial ora. Materials and Methods: Enterococcus faecalis, Candida albicans, Staphylococcus aureus, Streptococcus mutans, and Escherichia coli standard strains were used. Microorganisms were cultivated and used for the evaluation of the antimicrobial eect of the dierent concentrations of the C.limonum and calcium hydroxide using disc diusion and agar well diusion methods. Then MIC, MBC, and MFC values were recorded. Results: Wider inhibition zones were obtained for C.limonum essential oil compared to calcium hydroxide. In the agar well diusion method, while the widest inhibition zone was obtained for C.albicans, the narrowest inhibition zone was observed for E.faecalis for C.limonum. For calcium hydroxide, the widest inhibition zone was in C.albicans cultures, however, the narrowest inhibition zone was observed for S.mutans. In the agar disc diffusion method, the widest inhibition zone was observed for C.albicans, and the narrowest inhibition zone was spotted in S.aureus for Citrus limonum. For calcium hydroxide, while the widest inhibition zone again reached for C.albicans, the narrowest inhibition zone was found for E.faecalis. The lowest MIC and MBC value for Citrus limonum was observed in S.mutans. For calcium hydroxide, the lowest MIC and MBC values were recorded for S.aureus, E.coli, and S.mutans. Conclusion: Data in experimental conditions showed that C.limonum essential oil may be an alternative candidate, and superior to calcium hydroxide in terms of antimicrobial activity for all chosen members of intracanal ora.

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