Yusuf Kemal ARSLAN, Faruk KARAKEÇİLİ, Aysun YILMAZ, Murat KARA, Bülent DABANLIOĞLU

Genotype distribution in Hepatitis C patients admitted to Erzincan Mengücek Gazi Training and Research Hospital

Aim: We aimed to determine the genotype distribution in Hepatitis C patients who Parasitology applied to Erzincan Binali YildirimUniversity, Mengücek Gazi Training and Research Hospital. Hepatitis C virus, the only member of the genus Hepacivirus of Flaviviridaefamily, is responsible for 25%-40% of all liver diseases. Hepatitis C virus causes acute hepatitis C and chronic hepatitis C infections.Chronic hepatitis C infection causes cirrhosis, liver failure, hepatocellular cancerand liver diseases in terminal periods.Material and Methods: A total of 77 patients, 41 male and 36 female, who were admitted to Erzincan Binali Yıldırım University,Mengücek Gazi Training and Research Hospital in the period of January 2013-June 2019, were included in our study. RT-PCR andDNA sequencing for the 5’UTR region of the HCV genome for HCV genotyping was performed using the ABI Prism 3130 × 1 DNASequencer device.Results: Sex of a total of 77 patients included in the study; 41 of them are men (53.2%) and 36 are women (46.8%). Average age of thepatients; It was 59.9 ± 16.6 (minimum 20 and maximum 94). HCV G1b (80.5%) in 62 patients, HCV G1a (7.8%) in 6, HCV G3a (3.9%)in 3, HCV G3 (2.6%) in 2 and HCV G2 (2.6%) was found in 2 patients. HCV G1 (1.3%) was detected in 1, HCV G4 (1.3%) in 1 patient.Conclusion: The dominant genotype in Hepatitis C patients who applied to Mengücek Gazi Training and Research Hospital wasdetermined as “HCV Genotype 1b”.

PDF

___

1. Choo Q, Kuo G, Weiner A, et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989;244:359-62.
2. World Health Organization.Monitoring and evaluation for viral hepatitis B and C: recommended indicators and framework. (Erişimtarihi:4.2016).
3. LiuCH, Liang CC, Liu CJ, et al. Pegylated interferon alfa-2a monotherapy for hemodialysis patients with acute hepatitis C. Clin Infect Dis 2010;51:541-9.
4. International Committee on Taxonomy of HCV genotypes and subtypes.https://talk.ictvonline. org/ictv_wikis/flaviviridae/w/sg_flavi/634/table1---confirmed-hcv-genotypes-subtypes-may- (Erişimtarihi: 8.5.2019).
5. World Health Organization. Hepatitis C.https://www. who.int/news-room/fact-sheets/detail/hepatitisc(Erişimtarihi: 9.7.2019).
6. BuschMP, Glynn SA, Stramer, SL, et al. A new strategy for estimating risks of Transfusion-transmitted viral infections based on rates of detection of recently infected donors. Transfusion 2005;45:254-64.
7. Shalmani HM, Ranjbar M, Alizadeh, AHM. Recommendations for prevention and control of hepatitis C virus (HCV) infection and HCV-related chronicdisease. J Liver 2013;3:889-2167
8. Alter MJ.Prevention of spread of hepatitis C. Hepatology 2002;36:93-8.
9. Shepard CW, Finelli L, Alter MJ.Global epidemiology of hepatitis Cvirus infection. Lancet 2005;5:558-67.
10. Yildirim B, Tahan V, Ozaras R. et al. Hepatitis C virus risk factors in the Turkish community. Dig Dis Sci 2005;50:2352-5. Ann Med Res 2020;27(4):1369-73
11. Caruntu FA, Benea L. Acute hepatitis C virus infection: Diagno¬sis, pathogenesis, treatment.Gastrointestin Liver Dis 2006;15:249-56.
12. World Health Organization. Guidelines for the Screening, Careand Treatment of Persons with Hepatitis C Infection.https://apps.who.int/iris/bitstream/ handle/10665/205035/9789241549615_eng. F7E027BBB8?sequence=1(Erişimtarihi:27.4.2016).
13. Altuglu I, Soyler I, Ozacar T, et al. Distribution of hepatitis C virus genotypes inpatients with chronic hepatitis C infection in Western Turkey. Int J Infect Dis 2008;12:239-44.
14. Gower E, Estes C, Blach S, et al. Global epidemiology and genotype distribution of the hepatitis C vi¬rus infection. J Hepatol 2014;61:45-57.
15. Gurbuz Y, Tutuncu EE, Tulek NE, et al. Evaluation of Dual Therapy in Real Life Setting in Treatment-Naïve Turkish Patients with HCV Infection: A Multicenter, Retrospective Study. Balkan Med J 2016;33:18-26.
16. Altuğlu I, Sertoz R, Aksoy A, et al.Possible transmission risks and genotype distribution of hepatitisC virus infection in Western Turkey. Turk J Gastroenterol 2013;24,349-55.
17. Aktas O, Ozbek A, Aydin H, et al. Distribution of HCV Genotypes in Patients of with Chronic Hepatitis C in the Eastern Anatolia Region. Viral Hepat J 2014;20:91- 4.
18. Ucbilek E, Abayli B, Koyuncu MB, et al. Distribution of hepatitis C virus genotypes among intravenous drug users in Çukurova region of Turkey. Turk J Med Sci 2016;46:66-71.
19. Akgun S, Tarhan G, Sayiner HS, et al.Determination of hepatitis C virus genotype distribution in Adıyaman province. Ortadoğu Tıp Dergisi 2017;9:1-5.
20. Guss D, Sherigar J, Rosen P, and Mohanty SR. Diagnosis and management of hepatitis C infection in primary care settings. J Gen Intern Med 2018;33:551- 7.
21. World Health Organization.Global Hepatitis Report 2017.http://https://www.who.int/hepatitis/ publications/global-hepatitis-report2017/en/ (Erişimtarihi: 21.4.2017).
22. Popping S, Bade D, Boucher C, et al. The global campaign to eliminate HBV and HCV infection: International Viral Hepatitis Elimination Meeting and core indicators for development towards the 2030 elimination goals. J Virus Erad 2019;5:60.
23. Chung RT, Ghany MG, Kim AY, et al. Hepatitis C guidance 2018 update: AASLD-IDSA recommendations for testing, managing, and treating hepatitis C virus infection. Clin Infect Dis 2018.
24. Abacioglu YH, Davidson F, Tuncer S, et al. The distribution of hepatitis C virus genotypes in Turkish patients. J Viral Hepat 1995;2:297-301.
25. Kuckoztas MF, Ozgunes N, Yazici S. Investigation of the relationship between hepatitis c virus (HCV) genotypes with HCV-RNA and alanine aminotransferase levels in chronic hepatitis c patients. Mikrobiyol Bul 2010;44:111-5.
26. Buruk CK, Bayramoglu G, Reis A, et al.Determination of Hepatitis Cvirus genotypes in the Eastern Black Sea region.Mikrobiyol Bul 2013;47:650-7.
27. Us T, Kasifoglu N, Aslan FG, et al. The Distribution of Hepatitis C Virus Genotypes of patients with Chronic Hepatitis C Infection in the Eskişehir Region of Turkey. J Clin Virol 2017;8:88-91.
28. Balin SO, Tartar AS, Akbulut A, et al. Distribution of Hepatitis C virüs Genotype in Elazığ and the relationship between HCV RNA and serum alanin aminotransferase levels with genotype. ANKEM 2017;31:48-52.
29. Karabulut N, Alacam S, Yolcu A, et al. Distribution of hepatitis C virus genotypes in Istanbul, Turkey. Indian J Med 2018;36:192.
30. Zeytinli UO, Yucel FM, Dincer SD, et al. Distribution of hepatitis C virus genotypes in the region of Istanbul Northern Anatolian Association of Public Hospitals. Viral Hepatit Dergisi 2017;23:10.
31. Oz S, Koroglu M, Ozbek A, et al.Hepatitis C Virus Genotype Distribution in Sakarya Province; ThreeYear Retrospective Study. Online Türk Sağlık Bilimleri Dergisi 2019;4:444-53.
32. Tuzuner U, Gulcen BS, Ozdemir M, et al.Seven year genotyp distribution among Hepatitis C patients in a city in the central Anatolia Region of Turkey.Viral Hepatit J 2018;24:12-7.
33. Agca H, Mistik R, and Kazak E. Distribution of Hepatitis C Virus Genotypes in the South Marmara Region. J Clinand Anal Med 2013;4,7.
34. Kayman T, Karakukcu C, Karaman A, et al. Genotype distribution of Hepatitis C virus infection in the Kayseri region.Türk Mikrobiyol Cem Derg 2012;42:21-6.
35. Gokahmetoglu S, Atalay MA, and Kilinc A. Determination of the Hepatitis C Virus Genotypes With “Pyrosequencing” Method. Erciyes Med J 2011;33:99- 102.
36. Kirisci O, Caliskan A, Kocturk SA, et al. The Relationship Between Distribution of HCV-RNA and ALT-AST Levels with Genotypes of Hepatitis C Virus Infected Patients. Viral Hepat J 2013;19:67-70.
37. Saglik I, Mutlu D, Ongut G, et al. Distribution of Hepatitis C Virus Genotypes among Patients with Chronic Hepatitis C Infection in Akdeniz University Hospital, Antalya, Turkey: A Five-Year Evaluation Mikrobiyol Bul 2014;48:429-37.
38. Borcak D, Cagir U, and Yalciner A. Serum wıth hepatıtıs c vırus genotype dıstrıbutıon ın Nevşehir provınce amınotransphase and quantıtatıve serum of the fıeld HCV RNA level relatıons. ANKEM 2015;29:36-40.
39. Lee YS, Yoon SK, Chung ES, et al. The relationship of histologic activity to serum ALT, HCV genotype and HCV RNA titers in chronic hepatitis C. J Korean Med Sci 2001;16:585.