Evaluation of the time-dependent effect of an enzymatic denture cleanser tablet against six microbial species

Aim: In elderly individuals, infection control is, essential, especially in the presence of severe bacteria such as oral environment.Prevention of biofilm formation in removable dental prosthesis, which is often used in elderly patients might help infection control.This study aimed to evaluate the time-dependent effect of an enzymatic tablet on six microorganisms cultured in two differentdenture base resins.Material and Methods: Polymethylmethacrylate (PMMA) and polyamide-resin were used in this study. 480 samples were preparedfor each resin, and denture cleanser tablet was tested against C.albicans, S.mutans, S.gordonii, A.actinomycetemcomitans, S.aureus,and E.coli for 3rd, 5th, 10th, 20th, 40th, 80th, 160th, and 200th minutes. Cell viability (CV) was evaluated by MTT. ANOVA was used forstatistics.Results: C.albicans exhibited higher CV in polyamide-resin compared to PMMA-resin in all tested durations. S.gordonii andA.actinomycetemcomitans exhibited higher CV in PMMA-resin except for 80th minute-S.gordonii, which had similar values. S.mutanshad a higher CV in PMMA-resin than polyamide-resin at 3rd, 5th, 10th, 160th, and 200th minutes, and other values were similar topolyamide-resin. The tested concentration killed more than 75% of microorganisms except for 3rd and 5th minute-C.albicans on resins.S.aureus and E.coli had similar. CV of microorganisms on PMMA-resin was higher than on polyamide-resin. All microorganismsexhibited different adherence on the resins.Conclusions: Tablet cleanser was effective in all microorganisms and durations and over 10-minute durations provided over 76% celldeath. Patients with a compromised immune system can safely use restorations composed of polyamide-resin or PMMA-resin with40 min-treatment of tablet cleanser.

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1. Ioannidis G, Paschalidis T, Petridis H, et al. The influence of age on tooth supported fixed prosthetic restoration longevity. A systematic review. J Dent 2010;38:173-81.
2. Kraft-Bodi E, Jørgensen M, Keller M, et al. Effect of probiotic bacteria on oral Candida in frail elderly. J Dent Res 2015;94:181S-6S.
3. Scannapieco FA, Shay K. Oral health disparities in older adults: oral bacteria, inflammation, and aspiration pneumonia. Dent Clin North Am 2014;58:771-82.
4. Duval X, Millot S, Chirouze C, et al. Oral streptococcal endocarditis, oral hygiene habits, and recent dental procedures: a case-control study. Clin Infect Dis 2017;64:1678-85.
5. Konig MF, Abusleme L, Reinholdt J, et al. Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid arthritis. Sci Transl Med 2016;8:369ra-176.
6. Aoyama N, Suzuki JI, Kumagai H, et al. Specific periodontopathic bacterial infection affects hypertension in male cardiovascular disease patients. Heart Vessels 2018;33:198-204.
7. Komaru Y, Ishioka K, Oda T, et al. Nephritis-associated plasmin receptor (NAPlr) positive glomerulonephritis caused by Aggregatibacter actinomycetemcomitans bacteremia: A case report. Clin Nephrol 2018;90:155-60.
8. Storms I, Van Den Brand M, Schneeberger P, et al. Aggregatibacter actinomycetemcomitans pneumonia with chest and abdominal wall involvement. BMJ Case Rep 2017;2017:bcr2016217377.
9. Gradel KO, Nielsen SL, Pedersen C, et al. Seasonal variation of Escherichia coli, Staphylococcus aureus, and Streptococcus pneumoniae bacteremia according to acquisition and patient characteristics: a population-based study. Infect Control Hosp Epidemiol 2016;37:946-53.
10. Abuzar MA, Bellur S, Duong N, et al. Evaluating surface roughness of a polyamide denture base material in comparison with poly (methyl methacrylate). J Oral Sci 2010;52:577-81.
11. Baena-Monroy T, Moreno-Maldonado V, Franco-Martinez F, et al. Candida albicans, Staphylococcus aureus and Streptococcus mutans colonization in patients wearing dental prosthesis. Med Oral Patol Oral Cir Bucal 2005;10:E27- 39.
12. Branting C, Sund ML, Linder LE. The influence of Streptococcus mutans on adhesion of Candida albicans to acrylic surfaces in vitro. Arch Oral Biol 1989;34:347-53.
13. Busscher HJ, Weerkamp AH, van der Mei HC, et al. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol 1984;48:980-83.
14. Barbeau J, Seguin J, Goulet JP, et al. Reassessing the presence of Candida albicans in denture-related stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:51-9.
15. Ferreira MA, Pereira-Cenci T, Rodrigues de Vasconcelos LM, et al. Efficacy of denture cleansers on denture liners contaminated with Candida species. Clin Oral Investig 2009;13:237-42.
16. Hahnel S, Rosentritt M, Burgers R, et al. Candida albicans biofilm formation on soft denture liners and efficacy of cleaning protocols. Gerodontology 2012;29:e383-91.
17. Kuramitsu HK, He X, Lux R, et al. Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 2007;71:653-70.
18. Teles FR, Teles RP, Sachdeo A, et al. Comparison of microbial changes in early redeveloping biofilms on natural teeth and dentures. J Periodontol 2012;83:1139-48.
19. Yasui M, Ryu M, Sakurai K, et al. Colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. Gerodontology 2012;29:e494-502.
20. Bamford CV, d’Mello A, Nobbs AH, et al. Streptococcus gordonii modulates Candida albicans biofilm formation through intergeneric communication. Infect Immun 2009;77:3696-704.
21. da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008;17:627-33.
22. de Freitas Fernandes FS, Pereira-Cenci T, da Silva WJ, Filho AP, et al. Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins. J Prosthet Dent 2011;105:51-8.
23. Freitas-Fernandes FS, Cavalcanti YW, Ricomini Filho AP, et al. Effect of daily use of an enzymatic denture cleanser on Candida albicans biofilms formed on polyamide and poly(methyl methacrylate) resins: an in vitro study. J Prosthet Dent 2014;112:1349-55.
24. Kulak OY, Kazazoglu E, Arikan A. Oral hygiene habits, denture cleanliness, presence of yeasts and stomatitis in elderly people J Oral Rehabil 2002;29:300-04.
25. Lucena-Ferreira SC, Ricomini-Filho AP, Silva WJ, et al. Influence of daily immersion in denture cleanser on multispecies biofilm. Clin Oral Investig 2014;18:2179-85.
26. van Winkelhoff AJ, Wolf JW. Actinobacillus actinomycetemcomitans-associated peri-implantitis in an edentulous patient. A case report. J Clin Periodontol 2000;27:531-35.
27. Sumi Y, Kagami H, Ohtsuka Yet al. High correlation between the bacterial species in denture plaque and pharyngeal microflora. Gerodontology 2003;20:84-7.
28. Sumi Y, Miura H, Michiwaki Y, et al. Colonization of dental plaque by respiratory pathogens in dependent elderly. Arch Gerontol Geriatr 2007;44:119-24.
29. Bezirtzoglou E, Cretoiu SM, Moldoveanu M, et al. A quantitative approach to the effectiveness of ozone against microbiota organisms colonizing toothbrushes. J Dent 2008;36:600-05.
30. de Souza RF, de Freitas Oliveira Paranhos H, Lovato da Silva CH, et al.Interventions for cleaning dentures in adults. Cochrane Database Syst Rev 2009:CD007395.
31. Hayran Y, Sarikaya I, Aydin A, et al. Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins. J Appl Oral Sci 2018;26:e20170077.
32. Karakis D, Akay C, Oncul B, et al. Effectiveness of disinfectants on the adherence of Candida albicans to denture base resins with different surface textures. J Oral Sci 2016;58:431-37.
33. Ozkan YK, Ucankale M, Ozcan M, et al. Effect of denture adhesive on the micro-organisms in vivo. Gerodontology 2012;29:9-16.
34. Nikawa H, Hamada T, Yamashiro H, et al. A review of in vitro and in vivo methods to evaluate the efficacy of denture cleansers. Int J Prosthodont 1999;12:153-59.
35. Vallittu PK, Miettinen V, Alakuijala P. Residual monomer content and its release into water from denture base materials. Dent Mater 1995;11:338-42.
36. Radford DR, Sweet S, Challacombe S, et al. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26:577-83.
37. Stafford G, Huggett R, MacGregor A, et al. The use of nylon as a denture-base material. J Dent 1986;14:18-22.
38. Kawara M, Iwata Y, Iwasaki M, et al. Scratch test of thermoplastic denture base resins for non-metal clasp dentures. J Prosthodont Res 2014;58:35-40.
39. Vojdani M, Giti R. Polyamide as a denture base material: A literature review. J Dent 2015;16:1-9.
40. Durkan R, Ayaz EA, Bagis B, et al. Comparative effects of denture cleansers on physical properties of polyamide and polymethyl methacrylate base polymers. Dent Mater J 2013;32:367-75.