Effects of caffeic acid phenethyl ester on root canal dentin: A SEM/EDX study

Effects of caffeic acid phenethyl ester on root canal dentin: A SEM/EDX study

Aim: To evaluate the effects of Caffeic Acid Phenethyl Ester (CAPE) and its combination with EDTA and NaOCl on the surface of rootcanal dentine.Material and Methods: Ninety human extracted central anterior teeth were instrumented. Teeth were divided into six groupsaccording to irrigation regime: (n=15). Group 0 (negative control) saline; group 1: 5.25% NaOCl/ distilled water (positive control);group 2: 0.5% CAPE; group 3: 5.25% NaOCl/17% EDTA; group 4: 5.25% NaOCl/0.5% CAPE and group 5: 0.5% CAPE/17% EDTA. Rootswere splitted longitudinally and examined under scanning electron microscopy. Energy Dispersive X-Ray (EDX) method was used toanalyse the root dentine mineral content.Results: A significant difference in smear layer (SR) was found between group 3 and group 4 (P0.05).Conclusions: In this study, CAPE did not result in better SR removal compared to NaOCl at all levels of the root canals. Overall, Group5 was as effective as group 3 in SR removal. The mineral content of root dentin has changed.

___

  • 1. Violich D, Chandler N. The smear layer in endodontics–a review. Inter Endod J 2010;43:2-15.
  • 2. Jain H, Rao S, Johnson AD, et al. Evaluation of SmearOFF, maleic acid and two EDTA preparations in smear layer removal from root canal dentin AU - Ballal, Nidambur Vasudev. Acta Odontol Scand 2019;77:28- 32.
  • 3. Nassar M, Hiraishi N, Tamura Y, et al. Phytic acid: An alternative root canal chelating agent. J Endod 2015;4:242-7.
  • 4. Dutner J, Mines P, Anderson A. Irrigation trends among American Association of Endodontists members: a web-based survey. J Endod 2012;38:37-40.
  • 5. Andrabi SM-U-N, Kumar A, Tewari RK, et al. An in vitro SEM study on the effectiveness of smear layer removal of four different irrigations. Iran EndodJ 2012;7:171.
  • 6. Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod 2002;28:17-9.
  • 7. Grawehr M, Sener B, Waltimo T, et al. Interactions of ethylenediamine tetraacetic acid with sodium hypochlorite in aqueous solutions. Inter Endod J 2003;36:411-5.
  • 8. Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Inter Dent J 2008;58:329-41.
  • 9. Ok E, Adanir N, Ozturk T. Antibacterial and smear layer removal capability of oregano extract solution. Eur J Dent 2015;9:20-4.
  • 10. Mehmet G, Gunay A, Tanik A. An evaluation of the effects of caffeic acid phenethyl ester and Ankaferd blood stopper on secondary wound healing of oral mucosal tissue. Turk J Med Sci 2020;50:248-57.
  • 11. Ucan MC, Koparal M, Agacayak S, et al. Influence of caffeic acid phenethyl ester on bone healing in a rat model. J Int Med Res 2013;41:1648-54.
  • 12. Gunay A, Arpag OF, Atilgan S, et al. Effects of caffeic acid phenethyl ester on palatal mucosal defects and tooth extraction sockets. Drug Des Devel Ther 2014;8:2069-74.
  • 13. Arasoglu T, Derman S, Mansuroglu B. Comparative evaluation of antibacterial activity of caffeic acid phenethyl ester and PLGA nanoparticle formulation by different methods. Nanotechnol 2016;15;27:025103.
  • 14. Pedrosa VO, Franca FMG, Turssi CP, Amaral F, Teixeira LN, Martinez EF, et al. Effects of caffeic acid phenethyl ester application on dentin MMP-2, stability of bond strength and failure mode of total-etch and self-etch adhesive systems. Arch Oral Biol 2018;94:16-26.
  • 15. Spano JC, Silva RG, Guedes DF, et al. Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod 2009;35:727-30.
  • 16. Buldur B, Kapdan A. Comparison of the EndoVac system and conventional needle irrigation on removal of the smear layer in primary molar root canals. Niger J Clin Pract 2017;20:1168-74.
  • 17. Hulsmann M, Rummelin C, Schäfers F. Root canal cleanliness after preparation with different endodontic handpieces and hand instruments: a comparative SEM investigation. J Endod 1997;23:301-6.
  • 18. Basrani BM, Gevik Update of Endodontic Irrigating Solutions. In: Basrani B, editor. Endodontic Irrigation Chemical Disinfection of the Root Canal System: Springer 2015;100-1.
  • 19. Qian W, Shen Y, Haapasalo M. Quantitative analysis of the effect of irrigant solution sequences on dentin erosion. J Endod 2011;37:1437-41.
  • 20. Sen BH, Erturk O, Pişkin B. The effect of different concentrations of EDTA on instrumented root canal walls. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:622-7.
  • 21. Tosun S, Karataslioglu E. Influence of Trigonella foenum graecum seed extract as root canal irrigation agent on root surfaces: A scanning electron microscope/energy dispersive X-ray analysis study. Micros Res Tech 2019;82:1975-81.
  • 22. Dundar M, Ozcan M, Comlekoglu M, et al.Nanoleakage inhibition within hybrid layer using new protective chemicals and their effect on adhesion. J Dent Res 2011;90:93-8.
  • 23. Nogo-Zivanovic D, Bjelovic L, Ivanovic V, Kanjevac T, Tanaskovic I. Consideration of the Therapeutic Potential of Irrigants in Endodontic Therapy. Serbian J Exper Clin Res 2018;19:103-12.
  • 24. Torabinejad M, Khademi AA, Babagoli J, Cho Y, Johnson WB, Bozhilov K, et al. A new solution for the removal of the smear layer. J Endod 2003;29:170-5.
  • 25. Sayin TC, Cehreli ZC, Deniz D, Aet al. Time-dependent decalcifying effects of endodontic irrigants with antibacterial properties. J Endod 2009;35:280-3.
  • 26. Sayin TC, Serper A, Cehreli ZC, et al. Calcium loss from root canal dentin following EDTA, EGTA, EDTAC, and tetracycline-HCl treatment with or without subsequent NaOCl irrigation. J Endod 2007;33:581-4.
  • 27. Kawasaki K, Ruben J, Stokroos I, et al. The remineralization of EDTA–treated human dentine. Caries Res 1999;33:275-80.
  • 28. Kandaswamy D, Venkateshbabu N. Root canal irrigants. J Conserv Dent 2010;13:256.
  • 29. Zhang K, Kim YK, Cadenaro M, et al. Effects of different exposure times and concentrations of sodium hypochlorite/ethylenediaminetetraacetic acid on the structural integrity of mineralized dentin. J Endod 2010;36:105-9.
  • 30. Turkun M, Kaya A. Effect of 10% sodium ascorbate on the shear bond strength of composite resin to bleached bovine enamel. J Oral Rehabil 2004;31:1184-91.
  • 31. Altundasar E, Ozcelik B, Cehreli ZC, et al. Ultramorphological and histochemical changes after ER, CR: YSGG laser irradiation and two different irrigation regimes. J Endod 2006;32:465-8.
  • 32. Topcuoglu HS, Koseoglu M. Effect of Er: YAG and Nd: YAG lasers on the mineral content of root canal dentin. Laser Med Sci 2015;30:809-13.
  • 33. Gurbuz T, Ozdemir Y, Kara N, et al. Evaluation of root canal dentin after Nd: YAG laser irradiation and treatment with five different irrigation solutions: a preliminary study. J Endod 2008;34:318-21.
Annals of Medical Research-Cover
  • Yayın Aralığı: 12
  • Yayıncı: İnönü Üniversitesi Tıp Fakültesi
Sayıdaki Diğer Makaleler

Monocyte/HDL ratio in sarcoidosis patients without treatment

Pınar YILDIZ, Mehmet Fatih ELVERİŞLİ

Methastatic N2 in T1 Non-small cell lug cancer: Should we go back to invasive staging?

Özgür Ömer YILDIZ, İlknur AYTEKİN ÇELİK

Epilepsy in the emergency department: Who needs hospitalization?

Miraç Aysen ÜNSAL, Ahmet ŞENEL

Evaluation of macular thickness and retinal nerve fiber layer thickness in pediatric patients with malnutrition

Mete GÜLER, Şükrü GÜNGÖR, Elif DAMAR GÜNGÖR, Nisa KAHRAMAN

Safe, easily applicable mini-laparotomic surgical steps in cornual ectopic pregnancy: Case report and demonstration of surgical technique

Zeynep BAYRAMOĞLU, Erhan OKUYAN, Çağlar ALTUNDAL

Comparison eCAP and behaviour thresholds in post lingual medel cochlear implant users

Hüseyin DENİZ, Elif Tuğba SARAÇ, Şemsettin OKUYUCU

Development, Validity and Reliability of the 4-point Likert Turkish version of Cognitive Failures Questionnaire

Hale YAPICI ESER, Ömer AYDEMİR, Merve YALÇINAY İNAN, Özge KILIÇ, Cenk ERCAN, Mehmet Utku KÜÇÜKER, Can Misel KILIÇSIZ, Sezen YILMAZ, Neris DİNÇER

Is lesser curvature resection for GIST can be associated with delayed gastric emptying?

Tamer ERTAN, Ömer TOPUZ, Yunus DÖNDER, Yusuf SEVİM, Mehmet BAYKAN, Saliha KARAGÖZ EREN

Does postimplantation syndrome due to endovascular aneurysm repair increase mortality rate?

Selen ÖZTÜRK, İbrahim ÖZTÜRK, Betül ŞEN, Habip YILMAZ

Influence of coronary artery dominance on long-term outcome in patients with non-obstructive coronary artery disease

Hasan Ali BARMAN