M Arif ALADAG, Azibe YILDIZ, Yusuf TURKOZ, Hakan PARLAKPINAR

Ratlarda Deneysel Subaraknoidal Kanama Sonrası Gelişen Serebral Vazospazmın, Seleritrin Kullanılarak Engellenmesi

Amaç: Önlenmesi için büyük çapta deneysel ve klinik araştırmalar yapılmış olmasına rağmen, anevrizmal subaraknoidal kanamanın SAK yıkıcı bir tıbbi komplikasyonu olan serebral vazospazm, yüksek oranda morbidite ve mortalite ile birliktedir. Deneysel sonuçların çoğu güçlü vazokonstrüktör protein kinaz C’nin PKC serebral vazospazmda anahtar bir rol oynadığı görüşünü desteklemektedir. Biz vazospazmı önlemek için, deneysel subaraknoidal kanama modelinde güçlü, selektif ve hücre içine geçebilen protein kinaz C PKC inhibitörünü seleritrin klorid sistemik olarak kullandık. Materyal ve Yöntem: Yirmi sekiz sıçan dört gruba ayrıldı: grup 1 kontrol grubu; grup 2, SAK grubu, grup 3, SAK artı plasebo grubu; ve grup 4, SAK artı chelerythrine chloride 5 µmol /kg/gün grubu ayrıldı. 3. ve 4. gruplara Seleritrin klorid veya eşit hacimde % 0.9 salin sırasıyla, intraperitoneal olarak 5 gün süreyle verildi. Sıçanlar beşinci günde sakrifiye edildi. Kesitler ışık mikroskobu ile incelendi. Pons seviyesinde yapılan kesitlerde, baziler arterin lümen alanı ve duvar kalınlığı ölçümleri mikrometre ile alındı. Bulgular: 4. gruptaki baziler arter daralması 2 ve 3 gruplarla karşılaştırıldığında, önemli ölçüde p

Anahtar Kelimeler:

Sıçan, Subaraknoidal Kanama, Vazospazm, PKC İnhibitörü

The Inhibition of Cerebral Vasospasm by Using Chelerythrine After Experimental Subarachnoidal Haemorrhage in Rats

Background: Cerebral vasospasm, a devastating medical complication of aneurysmal subarachnoid hemorrhage SAH , is associated with high morbidity and mortality although a great deal of experimental and clinical research conducted to prevent this complication. The most of the experimental results support the view that potent vasoconstrictor proteine kinase C PKC , plays a key factor in cerebral vasospasm. To inhibit vasospasm, we used a potent, selective, and cell-permeable protein kinase C PKC inhibitor chelerythrine chloride systemically in an experimental subarachnoidal hemorrhage SAH model. Methods: Twenty eight rats were divided into four groups: group 1; control, group 2; SAH, group 3; SAH plus placebo, and group 4; SAH plus chelerythrine chloride 5 µmol/kg/day . Chelerythrine chloride or an equal volume of 0.9 % saline was intraperitoneally administered for 5 days to groups 3 and 4, respectively. The rats were sacrificed on the fifth day. Sectioned slices were examined by light microscopy. Measurements were made for the cross-sectional areas of the lumen and the vessel wall of basilar artery in the sections of pons by a micrometer. Findings: The vasoconstriction of the basilar artery significantly attenuated in group 4 compared with the groups 2 and 3 p

Keywords:

Rat, Subarachnoid Haemorrhage, Vasospasm, PKC Inhibitor,

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