Osman KUTSAL, A.Kübra Terim KAPAKİN

Tavuklarda deneysel amiloid artropatinin ışık mikroskobik ve elektron mikroskobik incelenmesi

Bu çalışmada, beşi kontrol grubu olacak şekilde 45 adet 5 haftalık ISA Brown ırkı kahverengi yumurtacı tavuk kullanıldı. I. gruba Enterococcus faecalis’in EFAA-01 suşu, II. gruba ise Enterococcus faecalis’in EFTD-07 suşları intravenöz (v.ulnaris) yolla 0.1 ml, 109 CFU dozda, inokule edildi. 10 haftalık gözlemden sonra tavukların servikal dislokasyon yöntemi ile nekropsileri yapıldı. Bulgular klinik, makroskobik, ışık ve elektron mikroskobik yönden değerlendirildi. Yapılan değerlendirmeler sonucunda I. grup hayvanların eklemlerinde amiloid birikimleri gözlenirken, II. grup hayvanların eklemlerinde amiloid birikimi gözlenmedi. Bu sonuçlar doğrultusunda da her Enterococcus faecalis suşunun amiloid birikimine neden olmadığı görüldü.

Light microscopic and electron microscopic examination of experimental amyloid arthropathy in the chickens

In this study, a total of 45 layer chickens (ISA Brown) at age of 5 weeks were assigned one of three experimental groups: Control (n=5) and intravenous inoculation of EFAA-01 serotype of Enterococcus faecalis (Group I, n=20) and EFTD-07 serotype of Enterococcus faecalis (Group II, n=20) via V. ulnaris at 109 cfu (0.1 ml). After monitoring for 10 weeks, chickens were sacrificed by cervical dislocation for necropsy. Findings were evaluated clinically and macroscopically as well as under light- and electron-microscope. Amiloid deposition in joints of chickens in Group I was evident, but not in Group II. The results suggest that amiloid deposition in response to Enterococcus faecalis inoculation was serotype dependent.

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