Oğuz KUL, Yonca Betil KABAK, Tolga GÜVENÇ, M.Yavuz GÜLBAHAR, Ahmet DENİZ

Systemic toxoplasmosis in a kangaroo (Macropus sp.)

Bu çalışma, yerel hayvanat bahçesinde kronik diyare teşhisinden sonra ölen bir çalı kangurusunda (Macropus sp.) sistemik toxoplazmozisi tanımlanmaktadır. Makroskopik olarak, deri altında yaygın peteşiyal kanamalar, karın boşluğunda 100 ml kanlı sıvı, omentum ve yağ dokuda pek çok nodüler yapılar ile mide ve ince bağırsakların subserozal damarlarında dilatasyon gözlendi. Mikroskopik olarak, akciğerde nekrotik bronkointerstisyel pnömoni ve miyokardium, adrenal bezler ile mide ve ince bağırsakların tunika muskularisinde pyogranülomatöz yangı ve çok sayıda nekrotik alan belirlendi. Pankreas ve çevresindeki abdominal yağ dokuda pyogranülomatöz yangı ile birleşmiş multifokal asellüler nekroz vardı. Serbest ve /veya intrasellüler yerleşimli Toxoplasma gondii benzeri granüler yapılar nekrotik alanlar ile birlikte görüldü. Bu granüler yapılar ve nekrotik alanlar poliklonal anti-Toxoplasma gondii antikoru ile şiddetli immunoreaksiyon gösterirken, Neospora caninum ve Leishmania sp. için reaksiyon negatifti. Toxoplasma gondii B1 geninin 97 bp uzunluğunda spesifik bir bölgesine yapılan nested-PCR ile toksoplazmozisin teşhisi doğrulandı. Sonuç olarak, Türkiye’de local bir hayvanat bahçesindeki kanguruda sistemik toksoplazmozis vakası histopatolojik, immunoperoksidaz teknik ve PCR kullanılarak ilk kez teşhis edildi.

Bir kanguruda (Macropus sp.) sistemik toksoplasmozis

This paper describes systemic toxoplasmosis in a brush kangaroo died after diagnosed with chronic diarrhoea in a local zoo. Macroscopically, widespread subcutaneous petechial haemorrhagies, 100 ml sero-sanguineous fluid in the abdominal cavity, many nodular structures on omentum and fat tissues with dilatation in subserosal vessels of gastric and small intestines were observed. Microscopically, necrotic bronchointerstitial pneumonia in the lungs and multiple areas of necroses with pyogranulomatous inflammation in the myocardium, adrenal glands and tunica muscularis of stomach and small intestines were detected. The pancreas and surrounding abdominal adipose tissues had multifocal coalescing acellular necroses conjoined with pyogranulomatous inflammation. Free and/or intracellularly located Toxoplasma gondii-like granular structures showed coassociation with necrotic lesions. These granular structures and necrotic areas exhibited strong immunoreactivity to polyclonal anti- T.gondii antibodies whereas reaction was negative for Neospora caninum and Leishmania sp.. Nested-PCR designed to amplify a 97 bp long specific part in B1 gene of T. gondii gave positive results. In conclusion, the first case of systemic toxoplasmosis in a kangaroo from a local zoo in Turkey was diagnosed by detailed histochemical, immunoperoxidase technique and PCR.

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