Ali BELGE, Büşra KİBAR KURT

Investigation of the effectiveness of dehydrated corneal collagen barriers on corneal defects: An experimental rabbit model

The aim of this study was to evaluate the effect of collagen shield on epithelial wound healing in rabbit eyes. Adult New Zealand Albino rabbits were used in the study. All surgical procedures were carried out under general anesthesia. Superficial keratectomies of 6 mm in diameter were created in 40 eyes of 20 rabbits and they were separated into 3 groups as the control (CN), medical treatment (CA) and collagen barrier (CB) groups. In the CN group, 6 rabbits received 0.9% NaCl drops. In the CA group, 7 rabbits received ciprofloxacin and acetylcysteine. In the CB group, a collagen shield was placed on corneal defect for 72 hours in 7 rabbits. Central corneal thickness was measured using an ultrasound pachymeter. Corneal thickness was determined before and at 72 and 96 hours after surgery. There was a significant increase (CA group: P

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1. Atiba A, Wasfy T, Abdo W, et al (2015): Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats. Clin Ophthalmol, 9, 2019–2026.
2. Carlson EC, Wang IJ, Liu CY, et al (2003): Altered KSPG expression by keratocytes following corneal injury. Mo Vis,9, 615–623.
3. Catalanotti P, Lanza M, Del Prete A, et al (2005): SlimeproducingStaphylococcus epidermidis and S. aureus in acute bacterial conjunctivitis in soft contact lens wearers. New Microbiol, 28, 345–354.
4. Cintron C, Kublin CL, Covington H (1981): Quantitative studies of corneal epithelial wound healing in rabbits. Curr Eye Res, 1, 507-516.
5. Eshar D, Wyre NR, Schoster J V (2011): Use of collagen shields for treatment of chronic bilateral corneal ulcers in a pet rabbit. J Small Ani Pract, 52, 380–383.
6. Fan T, Zhao J, Hu X, et al (2011): Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy. J Zhejiang Univ Sci B, 12, 492–498.
7. Frantz JM, Dupuy BM, Kaufman HE, et al (1989): The effect of collagen shields on epithelial wound healing in rabbits. Am J Ophthalmol, 108, 524–528.
8. Fu Y, Fan X, Chen P, et al (2010): Reconstruction of a tissue-engineered cornea with porcine corneal acellular matrix as the scaffold. Cells Tissues Organs, 191, 193–202.
9. Gasset AR, Kaufman HE (1970): Therapeutic uses of hydrophilic contact lenses. Am J Ophthalmol, 69, 252–259.
10. Gelatt KN, Gilger BC, Kern TJ (2013): Veterinary Ophthalmology. John Wiley&Sons, Inc., Iowa.
11. Günay C, Sağlıyan A, Yaman M (2005): Köpeklerde deneysel olarak oluşturulan korneal defektlerin sağaltımında asetilsisteinin etkisi. Fırat Üni Sağ Bil Vet Derg, 19, 151–156.
12. Honda N (2009): Descemet tripping automated endothelial keratoplasty using cultured corneal endothelial cells in a rabbit model. Arch Ophthalmol, 127, 1321.
13. Kanao S, Kouzuki S, Tsuruno M, et al (1993): Clinical application of 3% N-acetylcysteine collyrium on canine corneal diseases. J Japan Vet Med Assoc, 46, 487–491.
14. Klyce FD Beuerman RW (1988): Structure and function of the cornea. 3-54. In: HE Kaufman, BA Barron, MB McDonald (Eds), The Cornea. Churchill Livingstone, New York.
15. Kuwabara T, Perkins DG, Cogan DG (1976): Sliding of the epithelium in experimental corneal wounds. Invest Ophthalmol, 15, 4–14.
16. La Croix NC, Van Der Woerdt A, Olivero DK (2001): Nonhealing corneal ulcers in cats: 29 cases (1991-1999). J Am Vet Med Assoc, 218, 733–735.
17. Lesar TS, Fiscella RG (1985): Antimicrobial drug delivery to the eye. Drug Intell Clin Pharm, 19, 642–654.
18. Marmer RH (1988): Therapeutic and protective properties of the corneal collagen shield. J Cataract Refract Surg, 14, 496–499.
19. Marsich MM, Bullimore MA (2000): The repeatability of corneal thickness measures. Cornea, 19, 792–795.
20. Martin CL, Pickett JP (2019): Ophthalmic Disease In Veterinary Medicine. CRC Press/Taylor & Francis Group, New York.
21. Meek KM, Knupp C (2015): Corneal structure and transparency. Prog Retin Eye Res, 49, 1–16.
22. Mimura T, Yamagami S, Yokoo S, et al (2005): Sphere therapy for corneal endothelium deficiency in a rabbit model. Invest Opthalmol Vis Sci, 46, 3128.
23. Nagata M, Nakamura T, Hata Y, et al (2015): JBP485 promotes corneal epithelial wound healing. Sci Rep, 5, 14776.
24. Nakamura Y, Nakamura T, Tarui T, et al (2012): Functional role of PPARδ in corneal epithelial wound healing. Am J Pathol, 180, 583–598.
25. Oltulu R, Şatirtav G, Donbaloğlu M, et al (2014): Intraoperative corneal thickness monitoring during corneal collagen cross-linking with ısotonic riboflavin solution with and without dextran. Cornea, 33, 1164–1167.
26. Perran G (1989): Karnivorlarda (köpek ve kedi) ulkus kornea olgularının sagaltımında subkonjunktival alfakimotripsin enzimi uygulamaları. Ankara Univ Vet Fak Derg, 36, 704-721.
27. Poland DE, Kaufman HE (1988): Clinical uses of collagen shields. J Cataract Refract Surg, 14, 489–491.
28. Robin JB, Keys CL, Kaminski LA, et al (1990): The effect of collagen shields on rabbit corneal reepithelialization after chemical debridement. Invest Ophthalmol Vis Sci, 31, 1294-1300.
29. Schulz D, Iliev ME, Frueh BE, et al (2003): In vivo pachymetry in normal eyes of rats, mice and rabbits with the optical low coherence reflectometer. Vision Res, 43, 723–728.
30. Shaker GJ, Ueda S, LoCascio JA, et al (1989): Effect of a collagen shield on cat corneal epithelial wound healing. Invest Ophthalmol Vis Sci, 30, 1565–68.
31. Sharma S (2011): Antibiotic resistance in ocular bacterial pathogens. Indian J Med Microbiol, 29, 218–222.
32. Simsek NA, Ay GM, Tugal-Tutkun I, et al (1996): An experimental study on the effect of collagen shields and therapeutic contact lenses on corneal wound healing. Cornea, 15, 612–616.
33. Stoop JWFM (1970): Treatment of pressure sores in paraplegic patients with animal collagen. Spinal Cord, 8, 177–182.
34. Willoughby CE, Batterbury M, Kaye SB (2002): Collagen corneal shields. Surv Ophthalmol, 47, 174–182.