Neziha HACIHASANOĞLU ÇAKMAK, Hasret YARDİBİ

Holştayn ırkı sığırlarda sığır lökosit bağlanma yetmezliği, faktör XI eksikliği ve kompleks vertebral malformasyon hastalıkları genlerinin allel ve genotip sıklıklarının tespit edilmesi

Kalıtsal hastalıklar verim ve ekonomik kayıplara neden olur. Kalıtsal hastalıkları moleküler düzeyde incelemek, hastalıkları sürüden uzaklaştırmak için önemlidir. Araştırmamızda, Holstayn ırkı sığırlarda sığır lökosit bağlanma yetmezliği, faktör XI eksikliği ve kompleks vertebral malformasyon hastalıklarına sebep olan genlerin allel sıklıklarının tespit edilmesi amaçlanmıştır. Kocaeli, Sakarya ve Balıkesir illerinde bulunan farklı çiftliklerdeki 300 adet Holştayn sığırdan kan örnekleri alınmıştır. Kan örneklerinden standart amonyum asetat çöktürme yöntemiyle deoksiribonükleik asit örnekleri izole edilmiştir. Sığır lökosit bağlanma yetmezliği, faktör XI eksikliği ve kompleks vertebral malformasyona sebep olan mutant allelleri belirlemek için hedef bölgeler polimeraz zincir reaksiyonu ile çoğaltılmıştır. Sığır lökosit bağlanma yetmezliği için dizin analizi işlemine tabi tutulan örneklerin nükleotid kromotogramlarına göre toplamda 300 adet sığırdan 4 tanesi heterozigot birey, 296 tanesinin homozigot birey olduğu tespit edilmiştir. Faktör XI eksikliği hastalığı için polimeraz zincir reaksiyonu işlemi yeterli olup, kompleks vertebral malformasyon hastalığı için ise polimeraz zincir reaksiyonu ile çoğaltılan örnekler restriksiyon parçacık uzunluğu polimorfizmi işlemine tabi tutulmuştur. Faktör XI eksikliği ve kompleks vertebral malformasyon hastalıkları genlerinin hepsi homozigot normal bulunmuştur.

Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Hereditary diseases cause yield and economic loses. It is important to examine hereditary diseases at the molecular level and to remove diseases from the herd. In our study, it was aimed to determine allele frequencies of genes that cause bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation diseases in Holstein cattle. Blood samples were randomly taken from 300 Holstein cattle in different dairy farms in Kocaeli, Sakarya and Balıkesir provinces. Deoxyribonucleic acid samples were isolated from blood samples by using the standard ammonium acetate salt-out method. The target regions were amplified by polymerase chain reaction to determine the mutant alleles causing bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation. According to the nucleotide chromotograms of the samples subjected to bovine leukocyte adhesion deficiency analysis, it was determined that 4 out of 300 cattle were heterozygous and 296 were homozygous. Polymerase chain reaction procedure for factor XI deficiency disease was sufficient, while samples amplified by polymerase chain reaction for complex vertebral malformation disease were subjected to restriction particle length polymorphism. Factor XI deficiency and complex vertebral malformation disease genes were all homozygous normal.

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