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Hindi ve tavuklarda avian pneumovirus infeksiyonlarının indirek floresan antikor testi ve reverse transkriptaz-polimeraz zincir reaksiyonu ile teşhisi

Bu çalışmada Türkiye'de solunum problemi bulunan tavuk ve hindi sürülerinde Avian pneumovirus (APV) infeksiyonun varlığının belirlenmesi ve sonuçlarının karşılaştırmalı olarak değerlendirilmesi amaçlandı. Çalışmada 50 tavuk ve 20 hindi sürüsünden toplanan nazal turbinat, nazal svab akciğer ve trakea örnekleri indirek floresan antiköf testi (IFAT) ve reversetranskriptaz-polimeraz zincir reaksiyonu (RT-PCR) teknikleri ile incelendi. RT-PCR ile yapılan çalışmalarda, incelenen tavuk sürülerinin %46'sında ve hindi kümeslerinin %50'sinde APV genomu belirlendi. IF AT ile tavuk kümeslerinin %38'inde ve hindi kümeslerinin %35'inde APV antijenlerinin varlığı saptandı. RT-PCR ile incelenen teşhis materyalleri içinde tavuklarda en yüksek pozitiflik %50 ile nazal turbinatta ve %44.1 ile nazal svabta, hindilerde ise %57.1 ile nazal svabta ve %33.3 ile nazal turbinatta belirlendi. IF AT ile en yüksek pozitiflik aynı materyallerde fakat daha düşük oranlarda tespit edildi. Her iki teknikte de, nazal svab ve nazal turbinatlardan alınan materyallerin akciğer ve trakea örneklerine göre daha uygun olduğu saptandı. APV tanısında RT-PCR'ın IFAT'a göre daha duyarlı olduğu, kullanılan organın ve infeksiyon döneminin sonuçlara etki edebileceği kanısına varıldı.

Detection of avian pneumovirus infection in chickens and turkeys by indirect fluorescent antibody test and reverse transcriptase-polymerase chain reaction

The aim of this study was to detect the avian pneumovirus (APV) infection in chicken and turkey flocks with respiratory problems in Turkey. Nasal turbinate, nasal swab, lung and trachea samples collected from 50 chicken and 20 turkey flocks were examined by indirect fluorescent antibody test (IFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques in the study. APV genome was detected in 46% of chicken flocks and and 50% of turkey flocks by RT-PGR.In IFAT, APV antigens was determined in 38% of chicken flocks and 35% of turkey flocks. On the basis of diagnostic materials used in RT-PCR were considered, the highest positivities were detected in nasal turbinates (50%) and nasal swabs (44.1 %) of chickens; and in nazal swabs (57.1%) and nasal turbinates (33.3%) of turkeys. IFAT detected the highest positivity for APV infection in same materials with relatively lower percentages. In both techniques, nasal turbinates and nasal swabs were considered to be superior to the lung and tracheal samples for diagnosis of APV. In conclusion, it was suggested that RT-PCR was more sensitive than IFAT for the detection of APV, but test results might be affected by the test material and the time of infection.

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