Zeynep PEKCAN, M. Ziynet YILDIRIM, Sevil Atalay VURAL, Sibel E. Bilgihan OZTURK, Osman KUTSAL

Dermal squamous cell carcinoma (avian keratoacanthoma) in parakeets

Tümör şüpheli sekiz muhabbet kuşundan alınmış operasyon materyalleri klinik, patolojik ve immunhistokimyasal olarak değerlendirildi. İncelenen muhabbet kuşları 7ay- 9 yaş arasındaydı ve dördü erkek, biri dişi iken üç tanesinin cinsiyeti bildirilmemişti. Operasyon materyallerinin altısı kuyruk üzerinden ikisi ise kanat altından alınmıştı. İncelenen tümörlerin sert kıvamlı ve kesit yüzlerinin ise beyazımtırak renkte olduğu gözlendi. Tümöral kitlelerin histopatolojik incelenmesinde; dermiste hiperkromatik çekirdekli, büyük, ovoid şekilli, solgun eozinofilik sitoplazmalı hücrelerin gruplar oluşturduğu dikkati çekti. Bu hücre gruplarının bir çoğunun orta kısımlarında keratinize glob korne yapıları belirlendi. Glob korne yapıları içerisinde hiperkeratotik ve parakeratotik değişiklikler ile kalsifikasyonlara rastlandı. Olguların bazılarında dermiste nötrofil lökosit, lenfosit ve plazma hücrelerinden oluşan yangısal alanlar görüldü. Ayrıca bir olguda, saptanan tümörden başka aynı hayvandan alınan kitlelerin histopatolojik incelenmesinde ise; ortada koagulasyon nekrozu alanının etrafında heterofil lökositler, histiyositler, epiteloid hücreler, lenfositler ve yabancı cisim dev hücrelerinin oluşturduğu tüberkül yapısına rastlandı. Yapılan Ziehl-Neelsen boyamasında kırmızı renkli, çomak şekilli bakteri kümeleri görüldü. Mitotik aktiviteleri ve malignitelerini yorumlamak üzere dokular PCNA ve p53 proteinleri ile immunhistokimyasal olarak boyandı. Apoptozisin değerlendirilmesi için TUNEL metodu uygulandı.

Muhabbet kuşlarında dermal yassı hücreli kanser (keratoacanthoma)

Tumor suspected operation materials obtained from eight parakeets are evaluated clinically, pathologically and immunohistochemically. The age of the parakeets varied from seven months to nine years old. Four were male, one was a female and the sexes of the rest three were not identified. Six of the operation materials were taken from the above the tail while the other two from beneath the wings. Evaluated tumors were observed to be hard in consistency and whitish color on cut surface. At histopathological examination; it is seen that hyperchromatic nucleated, big, ovoid shaped, pale eosinophilic cytoplasmated cells had formed groups in dermis. Keratinized horn pearl formations are identified in the centers of most of these cell groups. Within the horn pearl formations hyperkeratotic, parakeratotic changes and calcifications are found. In some cases inflammation areas consisting of heterophil leukocytes, lymphocytes and plasma cells in the dermis are observed. Also in histopathological examination of one case, tubercle formations made of coagulation necrosis in the center surrounded by heterophil leukocytes, macrophages, epithelioid cells, lymphocytes and foreign body giant cells are found as well as tumoral formations. Groups of cudgel shaped, red colored bacteria are found in Ziehl-Neelsen staining of the sections. Tissues are stained for proliferating cell nuclear antigen (PCNA) and p53 proteins immunohistochemically in order to interpret mitotic activity and malignancy of the tumor. TUNEL method is applied to the tissues to evaluate apoptosis.

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