Tuba Berra SARITAŞ, Fatma GÖRÜCÜ, Yusuf KOÇ, Aziz BÜLBÜL, Zülfükar Kadir SARITAŞ, Musa KORKMAZ, H. Hüseyin DEMİREL

Anzer menşeli propolis örneklerinin sıçanların bağırsak kesisi üzerindeki yara iyileştirici etkisi

Bu çalışmada, Anzer menşeli propolis örneklerinin sıçanların deneysel bağırsak kesisi üzerindeki yara iyileştirici etkisinin araştırılması amaçlanmıştır. 250-300 gr ağırlığındaki toplam 34 erkek Wistar Albino sıçan beş gruba ayrıldı. Anzer propolis su solüsyonu (PW3 [n=6], PW7 [n=6]) ve Anzer propolisin etonolik solüsyonu (PE3 [n=6], PE7 [n=6]) uygulandı. Kontrol gruplarına (K3 [n=5], K7 [n=5]) tedavi uygulanmadı. Cerrahi prosedür (0. gün) genel anestezi altında laparotomi ile kolon enzize edildi ve dikiş uygulanarak kapatıldı. Fizyolojik tuzlu su ile seyreltilmiş Anzer propolis örneği PW3 ve PW7 gruplarına ameliyat öncesi ve sonrası 3 ve 7 gün süreyle gavaj (100 mg/kg/gün) ile uygulandı. Etanolde çözdürülmüş Anzer propolisi, PE3 ve PE7 gruplarına ameliyat öncesi ve sonrası 3 ve 7 gün sonda ile (100 mg /kg/gün) uygulandı. Deney hayvanlarına biyokimyasal ve histopatolojik değerlendirmeler için 3. ve 7. günlerde ötenazi yapılmıştır. Serum total oksidan seviye, total antioksidan seviye, interlökin-1, interlökin-6, tümör nekroz faktör-alfa, miyeloperoksidaz, hidroksiprolin ve nitrik oksit seviyeleri ELİSA yöntemi ile ölçüldü. Ensizyon hattı, yangı hücreleri, fibroblastik aktivite, neovaskülarizasyonu ve kollajen seviyesini değerlendirmek için örneklendi. Bu çalışma, etanolik Anzer propolis solüsyonunun oral uygulamasının, operasyon öncesi ve sonrası yedi gün boyunca bağırsak yara iyileşmesi üzerinde faydalı bir etkiye sahip olduğunu göstermektedir (PE7).

Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision

This study was investigated the wound healing effect of Anzer origin propolis specimens on rats’ experimental intestinal incisions. Thirty-four male Wistar albino rats have been divided into five groups, weighing 250-300 gr. Water solution of Anzer propolis (PW3 [n=6], PW7 [n=6]) or ethanolic solution of Anzer propolis (PE3 [n=6], PE7 [n=6]) were administered. Control groups (K3 [n=5], K7 [n=5]) received no treatment. The surgical procedure (day 0) consisted of a laparotomy under general anesthesia, during which the colon was incised and repaired with sutures. The Anzer propolis specimen diluted with physiologic saline was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PW3 and PW7 groups. Ethanol dissolved Anzer propolis was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PE3 and PE7. Experimental animals have been humanly euthanized on the 3rd and 7th days for biochemical and histopathological evaluations. Serum total oxidant status, total antioxidant status, interleukin-1, interleukin-6, tumor necrosis factor-alpha, myeloperoxidase, hydroxyproline and nitric oxide, levels were measured by ELISA. The incision line sampled to evaluate inflammatory cells, fibroblastic activity, neovascularization, and collagen level. The study indicates that the ethanolic Anzer propolis solution's oral administration has a beneficial effect on intestinal wound healing for seven days pre-and postoperatively (PE7).

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