Osman KUTSAL, Nihat YUMUŞAK

A comparative study between fine needle aspiration biopsy (FNAB) findings and histopathology in the evaluation of canine skin and skin adnexal tumors

Bu çalışmada, Ankara Üniversitesi Veteriner Fakültesi'ne ve Ankara'da bulunan özel veteriner kliniklerine deri tümörü şüphesiyle getirilen 43 köpeğe ait biyopsi örneği sitopatolojik ve histopatolojik olarak incelendi. Sitopatolojik örnekler sedasyon altında ince iğne aspirasyon biyopsisi (İİAB) tekniği ile alınarak May-Grünwald Giemsa (MGG) ile boyandı. Histopatolojik inceleme amacıyla alınan doku örnekleri %10'luk nötral formalin solüsyonunda tespit edilip, rutin doku takibine alınarak hematoksilen-eozin (H&E) ile boyandı. Tümörlerin alındığı köpeklerden 29'u erkek olup 14'ü ise dişiydi. Tümör görülme oranının 8-15 yaşları arasında arttığı gözlendi. Köpek ırkları incelendiğinde; köpeklerden 14'ü terrier, 14'ü melez ve 15'i diğer ırklardandı. Lokalizasyon olarak tümörler daha çok kafa, perianal bölge ve ön-arka bacaklarda yerleşim göstermekteydi. Sitopatolojik olarak toplam tümörlerden 30 (%69,76)'una malign epitelyal tümör, 5 (%11,62)'ine benign epitelyal tümör, 6 (%13,95)'sına epitelyal tümör şüpheli ve 2 (%4,65)'sine hafif malign epitelyal tümör tanısı konuldu. Histopatolojik olarak yapılan tanılar doğrultusunda ise tespit edilen tümörler; 12 (%27,9) yassı hücreli kanser, 9 (%20,9) hepatoid bez tümörü, 7 (%16,3) bazal hücreli kanser, 7 (%16,3) ter bezi tümörü, 5 (%11,6) sebaseöz bez tümörü, 2 (% 4,7) papillom ve 1 (%2,3) malignant pilomatrikom olarak belirlendi. Çalışma sonucunda sitopatolojik ve histopatolojik olarak yapılan tanıların karşılaştırmasında %86,05 oranında her iki tanının birbirleriyle örtüştükleri tespit edildi. Sonuç olarak, bu çalışma ile sitopatolojik tanı yönteminin köpeklerde deri tümörlerinin erken tanısında kullanılabileceği düşünülmektedir

Köpeklerde deri ve eklenti tümörlerinin değerlendirilmesinde ince iğne aspirasyon biyopsisi (İİAB) bulgularının histopatolojik bulgularla karşılaştırılması

The material of this study was, cytopathologic and histopathologic specimens obtained from 43 of dogs suffering from skin tumors. Samples were submitted by Ankara University, Faculty of Veterinary Medicine or private clinics of veterinary in Ankara. Cytologic specimens were prepared by fine needle aspiration biopsy (FNAB) technique and stained with May-Grünwald Giemsa (MGG). For histopathologic examinations tissue samples were obtained by surgery. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with Hematoxylin-Eosin (H&E). Tumors were obtained from 29 male dogs and 14 female dogs. The age with highest incidence in dogs was between 8 and 15. The breeds of dogs more frequently affected were 14 terrier, 14 mongrel and 15 another different breeds. Localizations of tumors were in the followed regions, head, perianal regions and legs. Cytopathologically, 30 (69,76%) malignant epithelial tumors, 5 (11,62%) benign epithelial tumors, 6 (13,95%) suspect epithelial tumors and 2 (4,65%) few malignant epithelial tumors were diagnosed. Histopathological diagnosis determined 12 (27,9%) squoamous cell carcinoma, 9 (20,9%) hepatoid gland tumours, 7 (16,3%) basal cell carcinoma, 7 (16,3%) sweat gland tumours, 5 (11,6%) sebaceous gland tumours, 2 (4,7%) papilloma and 1 (2,3%) malignant pilomatricoma. The diagnoses were in agreement cytopathologically and histopathologically in 86,05% of cases. The results obtained in the present study suggest that cytopathology is a practical tool for the early diagnosis of canine skin tumours

PDF

___

Berrocal A, Vos JH, Van Den Ingh Tsgam, et al. (1989): Canine perianal tumours. Zentralbl Veterinarmed A, 36, 739-749.
Bettini G, Morini M, Campagna F, et al. (2005): True grit: The tale of a subcutaneous mass in a dog. Vet Clin Pathol, 34, 73-75.
Bronden LB, Nielsen SS, Toft N, et al. (2010): Data from the Danish veterinary cancer registry on the occurrence and distribution of neoplasms in dogs in Denmark. Vet Rec, 166, 586-90.
Carroll EE, Fossey SL, Mangus LM, et al. (2010): Malignant pilomatricoma in 3 dogs. Vet Pathol, 47, 937- 943.
Daskalopoulou D, Maounis N, Kokalis G, et al. (1993): The role of fine needle aspiration cytology in the diagnosis of primary skin tumors. Arch Anal Cytol Path, 41, 75-81.
Dobson JM, Samuel S, Milstein H, et al. (2002): Canine neoplasia in the UK: estimates of incidence rates from a population of insured dogs. Small Anim Pract, 43, 240- 246.
Ghisleni G, Roccabianca P, Ceruti R, et al. (2006): Correlation between fine-needle aspiration cytology and histopathology in the evaluation of cutaneous and subcutaneous masses from dogs and cats. Vet Clin Pathol, 35, 24-30.
Goldschmidt MH, Dunstan RW, Stannard AA, et al. (1998): Histological classification of epithelial and melanocytic tumors of the skin of domestic animals. In: Schulman FY (ed.). WHO international histological classification of tumors of domestic animals, Armed Forces Institute of Pathology, Washington.
Goldschmidt MH, Hendrick MJ (2002): Tumors of the skin and soft tissues. In: Meuten DJ (ed.). Tumors in Domestic Animals. pp. 45-117, Iowa State Press, Iowa.
Goldschmidt MH, Thrall DE, Jeglum KA, et al. (1981): Malignant pilomatricoma in a dog. J Cutan Pathol, 8, 375- 381.
Griffiths GL, Lumsden JH, Valli V (1984): Fine-needle aspiration cytology and histologic correlation in canine tumors. Vet Clin Pathol, 13, 13-17.
Gulcubuk A, Gurel A (2003): 1995-2000 yılları arasında İstanbul'da saptanan köpek tümörleri. Istanbul Univ Vet Fak Derg, 29, 83-91.
Hall RL, Macwilliams PS (1988): The cytologic examination of cutaneous and subcutaneous masses. Semin Vet Med Surg (Small Anim), 3, 94.
Haziroglu R, Yardimci B, Aslan S, et al. (2010): Cytological evaluation of canine mammary tumours with fine needle aspiration biopsy technique. Revue Méd Vét, 161, 212-218.
Kaldrymidou H, Leontides L, Koutinas SF, et al. (2002): Prevalence, distribution and factors associated with the presence and the potential for malignancy of cutaneous neoplasms in 174 dogs admitted to a clinic in Northern Greece. J Vet Med A Physiol Pathol Clin Med, 49, 87-91.
Lakatos I, Baba AI, Catoi C, et al. (2008): Spontaneous skin tumors in dogs and cats, squamous carcinoma. Bulletin UASVM, 65, 51-54.
Macneill AL (2011): Cytology of canine and feline cutaneous and subcutaneous lesions and lymph nodes. Top Companion Anim Med, 26, 62-76.
Madewell BR, Theilen GH (1987): Tumors of the skin and subcutaneous tissues. Veterinary Cancer Medicine, Lea & Febiger, Philadelphia, 238-281.
Masserdotti C, Ubbiali FA (2002): Fine needle aspiration cytology of pilomatricoma in three dogs. Vet Clin Pathol, 31, 22-25.
Merlo DF, Rossi L, Pellegrino C, et al. (2008): Cancer incidence in pet dogs: Findings of the animal tumour registry of Genoa, Italy. J Vet Int Med, 22, 976-984.
Morrison WB, Denicola DB (1993): Advantages and disadvantages of cytology and histopathology for the diagnosis of cancer. Semin Vet Med Surg, 8, 222-227.
Nielsen SW, Aftosmis J (1964): Canine perianal gland tumors. J Am Vet Med Assoc, 144, 127.
Nielsen SW, Cole CR (1960): Cutaneous epithelial neoplasms of the dog, a report of 153 cases. Amer J Vet Res, 21, 931.
Pakhrin B, Kang MS, Bae IH, et al. (2007): Retrospective study of canine cutaneous tumors in Korea. J Vet Sci, 8, 229-236.
Rothwell TLW, Howlett CR, Middleton DJ, et al. (1987): Skin neoplasms of dogs in Sydney. Austr Vet J, 64, 161.
Sanja AK, Kukolj V, Marinkovic D, et al. (2005): Retrospective study of canine epithelial and melanocytic tumors. Acta Veterinaria (Beograd), 55, 319-326.
Scott DW, Anderson WI (1990): Canine sebaceous gland tumors: A retrospective analysis of 172 cases. Canine Pract, 15, 24-27.
Sharif MAM (2006): Epidemiology of skin tumors entities according to the new WHO classification in dogs and cats. VVB, Laufersweiler Verlag, Giessen, Germany.
Vascellari M, Baioni E, Ru G, et al. (2009): Animal tumour registry of two provinces in northern Italy: Incidence of spontaneous tumours in dogs and cats. BMC Vet Res, 5, 39.
Wellman ML (1990): The cytologic diagnosis of neoplasia. Vet Clin North Am Small Anim Prac, 20, 919- 937.
Yildirim F, Gurel A (2012): Comparison between cytological and histopathological evaluations of canine mammary tumours. Revue Méd Vét, 163, 116-122.