Figen DOĞU, Kenan KÖSE, Aydan İKİNCİOĞULLARI, Zehra Şule HASKOLOĞLU, Deniz GÜLOĞLU, Aylin ÇİFTÇİ, Funda ÇİPE, Ömer ÇİFTÇİ

Atopik Dermatitli Çocuklarda Regülatör T Hücreleri

Amaç: Bu çalışmanın amacı, atopik dermatitli (AD) çocuklarda periferik kanda CD4+CD25high+FOXP3+ regülatör T (Treg) hücreleri ve bu hücrelerin hastalığın patogenezi üzerine etkilerini değerlendirmektir. Gereç ve Yöntem: AD kriterlerini (Hanifin ve Rajka kriterlerini) karşılayan 41 AD’li hasta (yaş aralığı: 1-60 ay) ve 20 sağlıklı çocuk çalışmaya dahil edildi. CD4+CD25 + hücrelerde intrasitoplazmik FOXP3 ekspresyonu akım sitometrisi (Beckman Coulter FC500, USA, Software CXP 1.2) ile ölçüldü. Değerlendirmede FOXP3 molekülünün ortalama floresan yoğunluğunun (MFI) izotipik kontrolün MFI’ya göre değişimi esas alındı. Bulgular: AD’li çocukların kontrol grubuna göre; periferik kan Treg hücre seviyeleri düşük ve CD4+CD25high+FOXP3+Treg hücre MFI’ı daha yüksek bulundu (p

T Regulatory Cells in Children with Atopic Dermatitis

Objectives: The aim of this study was to examine peripheral blood CD4+CD25high+FOXP3+ regulatory T (Treg) cell levels in children with atopicdermatitis (AD) and to evaluate their effects on the pathogenesis of the disease.Materials and Methods: Forty one patients (age between 1-60 months) satisfied the criteria for AD (Hanifin and Rajka criteria) and 20 healthychildren were included into the study. Intracytoplasmic FOXP3 expression in CD4+CD25+ cells was measured by flow cytometry (Beckman CoulterFC500, USA, Software CXP 1.2). The change in mean fluorescence intensity (MFI) of the FOXP3 molecule relative to the MFI of the isotypic controlwas based on evaluation.Results: Children with atopic dermatitis have significantly lower levels of peripheral blood CD4+CD25high+FOXP3+ regulatory T cells and higherlevels of CD4+CD25high+FOXP3+ regulatory T cell MFI than the control group (p

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