Erdal DİNÇ, Feyyaz ONUR

3120

Simultaneous determination of atropine sulfate and morphine hydrochloride in their binary mixture using spectrophotometric methods

Bu çalışmada, atropin sülfat ve morfin hidroklorürün ikili karışımında aynı anda miktar tayinleri için dört spektrofotometrik yöntem kullanılmıştır. Birinci ve ikinci yöntemlerde, Vierordt ve modifiye Vierordt yöntemi, atropin sulfat ve morfin hidroklorürün miktar tayinleri bunların distile su içerisindeki çözeltilerinde 257.3 nm ve 284.4 nm lerde hesaplanmış olan A (% 1.1 cm) değerlerinden yararlanılarak gerçekleştirilmiştir. Üçüncü yöntemde, türev spektrofotometri, dA/dl değerleri her iki maddenin distile su içerisindeki çözeltilerinin birinci türev spektrumlarında 260.8 nm de atropin sülfat ve 244.7 nm de morfin hidroklorür için okunmuştur. Dördüncü yöntemde ise, spektrum oranları türev spektrofotometri, analitik sinyaller bu maddelerin distile su içerisindeki çözeltilerinde kendilerinin spektrumları bölücü olarak kullanılarak elde edilen bölüm spektrumlarının birinci türevinde 255.8nm de atropin sülfat için ve 273.6 nm de morfin hidroklorür için ölçülmüştür. Yöntemlerde hiçbir ayırma işlemi gerekmemektedir. Yöntemlerin ortalama geri kazanımları ve bağıl standart sapmaları hazırlanan sentetik karışımlarda hesaplanmıştır.

İkili karışımında atropin sulfat ve morfin hidroklorürün spektrofotometrik yöntemler kullanılarak aynı anda miktar tayinleri

In this study, four spectrophotometric methods are used for the simultaneous determination of atropine sulphate and morphine hydrochloride in their binary mixture. In the first and second methods, Vierordt's and modified Vierordt's methods, quantitation of atropine sulphate and morphine hydrochloride were realized by using A (% 1.1 cm) values determined at 257.3 nm and 284.4 nm in their solution in distilled water. In the third method, derivative spectrophotometry, dA/dl values were read at 260.8 nm for atropine sulphate and at 244.7 nm for morphine hydrochloride in the first derivative spectra of both compounds in their solution in distilled water. In the fourth method, ratio spectra derivative spectrophotometry, analytical signals were measured at 255.8 nm for atropine sulphate and 273.6 nm for morphine hydrochloride in the first derivative of ratio spectra obtained by using their spectra as divisor in their solution in distilled water. The procedures do not require any seperation step. Mean recoveries and relative standard deviations of the methods were calculated in synthetic mixtures.
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  • 1.Sichko A.I., "Phototurbidimetric titration of morphine hydrochloride and atropine sulfate" Farmatsiya, 27(3), 81-82 (1978) (CA 89, 33172g, 1978) ,
  • 2. Gao, M., Luo, J., "Determination of atropine in compound diphenoxylate tablets by ion-pair extraction spectrophotometry" Zhongguo Yiyao Gangye Zashi, 20(2), 78-81 (1989) (CA111,454095,1989)
  • 3. Hassan S.M., Davidson, A.G., "The assay of tropane derivatives in formulations by second derivative ultraviolet spectrophotometry" J.Pharm.Pharmacol., 36(1), 7-10 (1984)
  • 4. Karatodorov K., Kolarova, R.,"Extraction spectrophotometric method for the quantitative determination of atropine in Bulgarian patent drug forms" Izv.Durzh.Inst.Kont.LekSredstva, 11,24-29 (1978) (CA90, 7661 ld,1979)
  • 5. Korany, M.A., Mahgoup,H., "Spectrophotometric determination of atropine sulfate and nystatine in presence of each other drugs using orthogonal polinomials for an equal intervals" Pharmazie, 46(12), 883-885 (1991)
  • 6. Mahrous M.S., Abdelhamid, M.D., Dabees, H.G., Beltagy, Y.A., "Analysis of some selected non-UV absorbing drugs by first derivative spectrophotometry of their picrates" J.Pharm.Belg., 47(2), 135-140 (1992)
  • 7. Taha., A.M., Gomaa, C.S., "Spectrophotometric analysis of alkaloids with picrolonic acid" JAssoc.Anal.Chem., 59(3), 683-688 (1976)
  • 8. Lopez,A., Mazzeo, P., Quaglia, M.G., "Determination of morphine and heroine by second derivative UV spectrophotometry" Farmaco.Ed.Prat., 37(11), 371-376 (1982)
  • 9. Cieri U.R., "Determination of small quantities of atropine in commercial preparations in liquid chromatography" J.Assoc.Anal.Chem., 68(5), 1042-1045 (1985).
  • 10. Bettero, A. And Bollettin P., "Atropine sulfate determination by derivative spectroscopy or HPLC", Ann.Chim., 75(7-8), 251-258 (1985) (CA, 103,220892c, 1985)
  • 11. Jalal, I.M., "Determination of diphenoxylate hydrochloride and atropine sulfate in tablet formulations" Anal.Lett., 18(B20), 2551-68 (1985)
  • 12. Ceyhan,T., Kartal, M., Altun, M.L., Tulemis, F. And Cevheroğlu, S., "LC determination of atropine sulfate and scopolamine hydrobromide in pharmaceuticals" J.Pharm.Biomed.Anal., 25(3-4), 399-406 (2001)
  • 13. Lehr, G.J.,"Determination of diphenoxylate hydrochloride and atropine sulfate in combination drug formulations by liquid chromatography", J.AOAC, 79(6), 1288-1293 (1996)
  • 14. Longshaw, R.N., Walli, N., "Comparison of two methods of assay of selected eye drops" J.Hosp. Pharm., 31(2), 36-38 (1973)
  • 15. Aboutabl,E.A., Mossa J.S., Hassan,M.A., "pmr assay of natural products in pharmaceuticals. IV. Assay of codeine and simultaneous detection of morphine" Spectrosc.Lett., 12(7-8), 79-90 (1979)
  • 16. Vycudulik, W., Street,H.W., Machata,G., "Preparation of GC packed columns with low adsorption activity by heating the support material in vacuo prior to coating with substituted polysiloxane (SE-30): application to analysis of morphine and other polar drugs" J.Chromatogr.Sci, 21(9), 429-31 (1983)
  • 17. Mohamed, M.E. "First derivative spectrophotometric determination of a mixture of pirbuterol hydrochloride and butorphenol tartrate" Anal.Lett, 19(11-12), 1323-1339(1986)
  • 18. Çakır, B., Atay,0 and Tamer, U., "Quantitative determination of Hydrochlorothiazide and benazepril HC1 in pharmaceuticals using spectrophotometry and high performance liquid chromatography", J.Fac.Pharm. Gazi, 17(1), 43-52 (2000)
  • 19. Banoğlu, E., Özkan, Y. And Atay, O., "Dissolution tests of benazepril HC1 and hydrochlorothiazide in commercial tablets" IlFarmaco, 55(6-7), 477-483 (2000)
  • 20. Ivanovic, D., Medenica, M., Markovic, S. And Mandic, G., Second derivative assay of pseudoephedrine, ibuprofen and loratadine in pharmaceuticals, Arzneim.-Forsch., 50(II), 1004-1008 (2000)
  • 21. Rogic, D., "Simultaneous determination of acetylsalicylic and salicylic acid by first derivative spectrophotometry in pharmaceutical preparations, J.Molecular Structure, 294, 255-258(1993)
  • 22. Sayın, F., Kır, S. And Temizer, A., "Analysis of a syrup containing chlorpheniramine maleate, codeine phosphate and ephedrine hydrochloride by derivative spectrophotometry", FABAD J.Pharm.Sci, 21, 55-60 (1996)
  • 23. Yücesoy, C. And Erol S., "Simultaneous determination of medroxyprogesterone acetate and estradiol valerate by first derivative UV spectrophotometry", J.Fac.Pharm.Gazi. 11(1), 33-42 (1994)
  • 24. Salinas, F., Berzas Nevado J.J and Espinosa Maansilla A., "A new spectrophotometric method for quantative multicomponent analysis resolution of mixtures of salicylic and salicyluric acids" Talanta, 37, 347 - 351 (1990)
  • 25. Berzas Nevado, J.J., Lemus Gallego J.M. and Castenada Panalvo G. , "Spectra ratio derivative spectrophotomeric determination of sulphaquinoxaline pyrimethamine in veterinary formulation" J. Pharm. Biomed. Anal., 11, 601-607 (1993)
  • 26. Berzas Nevado J.J, Rodrigeuez Flores J. And Moreno Pardo M.L,. "Determination of sulphamethizole in the presence of nitrofurantoine by derivative spectrophotometry and ratio spectra derivative" Talanta, 38, 1261-1264 (1991)
  • 27. Berzas Nevado J.J, Rodrigeuez Flores J. And Moreno Pardo M.L, "Simultaneous determination of quinoline yellow and tartrazine by derivative spectrometry and ratio spectra derivative " Analusis , 21,395-401 (1993),
  • 28. Berzas Nevado, J.J., Lemus Gallego J.M. and Castenada Panalvo G. "Determination of sulfamethoxazole and trimethoprim by ratio spectra derivative spectrophotometry" Fresenius J.Anal. Chem., 342, 723 - 728 (1992),
  • 29. Dinç, E. And Onur, F., "Comparative study of the ratio spectra derivative spectrophotometry, derivative spectrophotometry and Vierordt's's method applied to the analysis of oxfendazole and oxyclonazide in a veterinary formulation" Analusis, 25, 55 -59 (1997)
  • 30. Dinç, E. And Onur, F., "Comparison of the ratio spectra derivative spectrophotometry, derivative spectrophotometry and Vierordt's's method applied to quantitative analysis of pseudoephedrine hydrochloride and triprolidine hydrochloride in tablets" STP Pharma Sci, 8(3),203- 208 (1998)
  • 31. Dinç, E. And Onur, F., "Application of the ratio spectra derivative spectrophotometric and Vierordt's's method to quantitative analysis of paracetamol and metamizol in a pharmaceutical formulation" Sci.Pharm., 67, 57 - 68 (1999)
  • 32.Dinç, E. And Onur, F., "Four new spectrophotometric methods for simultaneous determination of niclosamide and thiabendazole in tablets" Sci.Pharm., 64, 151 - 172 (1996)
  • 33. Dinç, E., Kökdil , G. And Onur, F. "A comparison of matrix resolution method, ratio spectra derivative spectrophotometry and HPLC for the determination of thiamine hydrochloride and pyridoxine hydrochloride in pharmaceutical preparation" J.. Pharm. Biomed.AnaL, 22(6), 915-923 (2000)
  • 34. Dinç, E., Kökdil , G. And Onur, F. " Derivative ratio spectra Zero-crossing spectrophotometry and HPLC method applied to the quantitative determination of paracetamol, propyphenazone and caffeine in ternary mixtures" J. Pharm. BiomedAnaL,26(5-6), 769-778 (2001)
  • 35. Dinç, E. And Onur, F ."Application of derivative and ratio derivative spectrophotometry for determination of pseudoephedrine and acrivastine" Anal.Lett., 30(6), 1179-1191 (1997)
  • 36. Onur, F., Yücesoy, C, Dermiş, S., Kartal, M. And Kökdil, G., "Simultaneous determination of pseudoephedrine sulfate, dexbrompheniramine maleate and loratadine in pharmaceutical preparation using derivative spectrophotometry and ratio spectra derivative spectrophotometry" Talanta, 51(2), 269-279 (2000)
  • 37. Dinç, E. And Onur, F. "Spectrophotometric determination of active ingredients in tablets containing meclizine hydrochloride" Sci.Pharm., 63, 113-126 (1995)
  • 38. Dinç, E. And Onur, F. "Simultaneous determination of caffeine and meclizine dihydrochloride in sugar-coated tablets" Anal.Lett, 28(14), 2521-2534 (1995)
  • 39. Dinç, E. And Onur, F ./'Simultaneous determination of cetrimide and chlorhexidine gluconate in antiseptic solutions by first derivative UV spectrophotometry" J.Fac.Pharm.Gazi, 11(2), 135-142 (1994)
  • 40. Dinç, E. And Onur, F .,'Simultaneous determination of caffeine and chlorphenoxamine hydrochloride in mixture by first derivative spectrophotometry" J.Fac.Pharm.Gazi, 12(1), 63-72 (1995)
Ankara Üniversitesi Eczacılık Fakültesi Dergisi-Cover
  • ISSN: 1015-3918
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2016
  • Yayıncı: Ankara Üniversitesi Eczacılık Fakültesi
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Sayıdaki Diğer Makaleler

Simultaneous determination of atropine sulfate and morphine hydrochloride in their binary mixture using spectrophotometric methods

Erdal DİNÇ, Feyyaz ONUR

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-net Daş EVCİMEN, George L. KING

SIMULTANEOUS DETERMINATION OF ATROPINE SULFATE AND MORPHINE HYDROCHLORIDE IN THEIR BINARY MIXTURE USING SPECTROPHOTOMERIC METHODS : İKİLİ KARIŞIMINDA ATROPİN SULFAT VE MORFİN HİDROKLORÜRÜN SPEKTROFO