Büşra CUMHUR, Sezen YILMAZ SARIALTIN, Safa GUMUSOK, Tülay COBAN, Ceyda Sibel KILIC

SAMBUCUS EBULUS L. BİTKİSİNİN FARKLI KISIMLARININ ANTİOKSİDAN VE ANTİ-ENFLAMATUVAR AKTİVİTELERİ

Amaç: Adoxaceae (Mürver) ailesinin Sambucus L. cinsi, önceki çalışmalarda antioksidan, anti-enflamatuar, antiviral ve antibakteriyel aktiviteleri nedeniyle araştırılmıştır. Ayrıca, yapraklarda yapılan önceki in vivo ve in vitro çalışmalar, bitkinin anti-enflamatuar aktiviteye sahip olduğunu göstermiştir. Çalışmamız, Sambucus ebulus L. bitkisinin kök, meyve ve yaprak ekstrelerinin in vitro antioksidan ve anti-enflamatuar potansiyelini araştırmayı amaçlamaktadır.Gereç ve Yöntem: Antioksidan aktivite, Sambucus ebulus kullanılarak biyolojik bir tahlilde değerlendirilirken, radikal süpürücü aktivite 2,2-difenil-1-pikrilhidrazil (DPPH) ve ABTS + yöntemleri kullanılarak ölçülmüştür.Sonuç ve Tartışma: Maksimum anti-enflamatuvar etki, sırasıyla yaprak ve meyve ekstrelerinin ardından kök ekstrelerinde gözlendi. Kök ekstreleri, anti-enflamatuvar aktivitenin sonuçlarına benzer şekilde en yüksek ABTS ve DPPH serbest radikal süpürme aktivitesini (FRSA) sergiledi. Sonuç olarak, kök ekstresi, FRSA ve antiinflamatuar aktivite açısından diğerleri arasında en güçlü ekstre olmuştur.

Anahtar Kelimeler:

Adoxaceae, anti-enflamatuvar aktivite, antioksidan aktivite, cüce mürver, Sambucus ebulus

ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF DIFFERENT PARTS OF SAMBUCUS EBULUS L.

Objective: Sambucus L. genus of Adoxaceae (Elderberry) family was investigated in previous studies for its antioxidant, anti-inflammatory, antiviral and antibacterial activities. Moreover, previous in vivo and in vitro studies performed on the leaves showed that the plant possesses anti-inflammatory activity. Our study aims to investigate the in vitro antioxidant and anti-inflammatory potential of the stem, fruit and leaf extracts of Sambucus ebulus L. plant. Material and Method: The antioxidant activity was evaluated in a biological assay using Sambucus ebulus, whereas the radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ABTS methods.Result and Discussion: The maximum anti-inflammatory effect was observed in stem extracts followed by leaf and fruit extracts, respectively. Stem extracts exhibited the highest ABTS and DPPH free radical scavenging activity (FRSA) similar to the results of anti-inflammatory activity. As a conclusion, stem extracts were the most potent extracts among the others regarding the FRSA and anti-inflammatory activities.In our study, the biological activity potential of the extracts was demonstrated, thus providing data supporting the traditional use of Sambucus ebulus L.

Keywords:

Adoxaceae, anti-inflammatory activity, antioxidant activity, dwarf elder, Sambucus ebulus,

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1. Jabbari, M., Daneshfard, B., Emtiazy, M., Khiveh, A., Hashempur, M.H. (2017). Biological effects and clinical applications of dwarf elder (Sambucus ebulus L). Journal of Evidence-Based Complementary & Alternative Medicin; 22(4), 996-1001. [CrossRef]
2. Chamberlain, D.F., Davis, P.H. (1972). Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh, p.540-41.
3. Seçmen, Ö., Gemici, Y., Görk, G., Bekat, L., Leblebici, E. (2000). Tohumlu Bitkiler Sistematiği, Ege Üniversitesi Basımevi, İzmir.
4. Ecevit-Genç, G., Özhatay, N. (2006). An ethnobotanical study in Çatalca (European part of Istanbul) II. Turkish Journal of Pharmaceutical Sciences, 3(2), 73-89.
5. Tasinov, O., Kiselova-Kaneva, Y., Ivanova, D. (2013). Sambucus ebulus - from traditional medicine to recent studies. Scripta Scientifica Medica, 45, 36-42. [CrossRef]
6. Sezik, E., Zor, M., Yesilada, E. (1992). Traditional medicine in Turkey II. Folk medicine in Kastamonu. International Journal of Pharmacognosy, 30(3), 233-239. [CrossRef]
7. Şimşek, I., Aytekin, F., Yeşilada, E., Yıldırımlı, Ş., Anadolu’da Halk Arasında Bitkilerin Kullanılış Amaçları Üzerinde Etnobotanik Bir Çalışma, 14. Bitkisel İlaç Hammaddeleri Toplantısı, Bildiriler, Başer K.H.C., Kırımer N., (Eds.), 29-31 Mayıs 2002, Eskişehir.
8. Yeşilada, E., Sezik, E., Honda, G., Takaishi, Y., Takeda, Y., Tanaka, T. (1999). Traditional medicine in Turkey IX: folk medicine in north-west Anatolia, Journal of Ethnopharmacology, 64, 195–210. [CrossRef]
9. Bulut, Y. (2006). Master Thesis. Manavgat (Antalya) Yöresinin Faydalı Bitkileri. Biyoloji Ana Bilim Dalı, Fen Bilimleri Enstitüsü, Süleyman Demirel Üniversitesi, Isparta, Türkiye.
10. Koçyiğit, M., Özhatay, N. (2006). Wild plants used as medicinal purpose in Yalova (Northwest Turkey). Turkish Journal of Pharmaceutical Sciences, 3(2), 91-103.
11. Tuzlaci, E., Işbilen-Alparslan, D.F., Bulut, G. (2010). Turkish Folk Medicinal Plants, VIII: Lalapaşa (Edirne). Marmara Pharmaceutical Journal, 14, 47-52. [CrossRef]
12. Tuzlaci, E., Tolon, E. (2000). Turkish folk medicinal plants, part III: Sile (Istanbul). Fitoterapia, 71, 673-685. [CrossRef]
13. Mustafa, B., Hajdari, A., Pieroni, A., Pulaj, B., Koro, X., Quave, C.L. (2015). A cross-cultural comparison of folk plant uses among Albanians, Bosniaks, Gorani and Turks living in south Kosovo. Journal of Ethnobiology and Ethnomedicine, 11(1), 1-26. [CrossRef]
14. Kültür, S. (2007). Medicinal plants used in Kırklareli Province (Turkey). Journal of Ethnopharmacology, 111(2), 341–364. [CrossRef]
15. Yeşilada, E. (1997). Evaluation of the anti-inflammatory activity of the Turkish medicinal plant Sambucus ebulus. Chemistry of Natural Compounds, 33, 539–540. [CrossRef]
16. Ahmadiani, A., Fereidoni, M., Semnanian, S., Kamalinejad, M., Saremi, S. (1998). Antinociceptive and anti-inflammatory effects of Sambucus ebulus rhizome extract in rats. Journal of Ethnopharmacology, 61(3), 229–235. [CrossRef]
17. Ebrahimzadeh, M.A., Mahmoudi, M., Salimi, E. (2006). Antiinflammatory activity of Sambucus ebulus hexane extracts. Fitoterapia, 77, 146–148. [CrossRef]
18. Yeşilada, E., Gurbuz, I., Shibata, H. (1999). Screening of Turkish anti-ulcerogenic folk remedies for anti-Helicobacter pylori activity. Journal of Ethnopharmacology, 66(3), 289–293. [CrossRef]
19. Martinez, M.J.A., Del Olmo, L.M.B., Benito, P.B. (2005). Antiviral activities of polysaccharides from natural sources. Studies in Natural Products Chemistry, 30, 393–418. [CrossRef]
20. Neto, C.C., Owens, C.W., Langfield, R.D., Comeau, A.B., Onge, J.S., Vaisberg, A.J., Hammond, G.B. (2002). Antibacterial activity of some Peruvian medicinal plants from the Callejon de Huaylas. Journal of Ethnopharmacology, 79, 133–138. [CrossRef]
21. Dawidowicz, A.L., Wianowska, D., Baraniak, B. (2006). The antioxidant properties of alcoholic extracts from Sambucus nigra L. (antioxidant properties of extracts). LWT-Food Science and Technology, 39(3), 308–315. [CrossRef]
22. Yeşilada, E., Ustun, O., Sezik, E., Takaishi, Y., Ono, Y., Honda, G. (1997). Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1alpha, interleukin-1beta and tumor necrosis factor alpha. Journal of Ethnopharmacology, 58(1), 59–73. [CrossRef]
23. Blois, M.S. (1958). Antioxidant determination by the use of a stable free radical. Nature, 181(4617), 1199–1200. [CrossRef]
24. Yalçin, C.Ö., Yilmaz Sarialtin, S., Çiçek Polat, D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. Journal of Research in Pharmacy, 24(4), 487-496. [CrossRef]
25. Carneiro, N.V.Q., Da Silva, H.B.F., Da Silva, R.R., Carneiro, T.C.B., Costa, R.S., Pires, A.O., Marques, C.R., Velozo, E.S., Conceição, A.S., Da Silva, T.M.S., Da Silva, T.M.G., Alcântara-Neves, N.M., Figueiredo, C.A. (2022), Sambucus australis modulates inflammatory response via inhibition of nuclear factor kappa B (NF-kB) in vitro. Anais da Academia Brasileira de Ciências 11(7), e12911729567. [CrossRef]
26. Bahiense, J.B., Marques, F.M., Figueira, M.M., Vargasa, T.S., Kondratyuk, T.P., Endringer, D.C., Scherer, R., Fronzaa, M. (2017). Potential anti-inflammatory, antioxidant and antimicrobial activities of Sambucus australis. Pharmaceutical Biology, 55(1), 991–997. [CrossRef]
27. Ali, E.M., Somayyeh, E., Bahman, E. (2009), Sambucus ebulus elburensis fruits: A good source for antioxidants. Pharmacognosy Magazine, 5(19), 213-218.
28. Ivanova, D., Tasinov, O., Kiselova-Kaneva, Y. (2014). Improved lipid profile and increased serum antioxidant capacity in healthy volunteers after Sambucus ebulus L. fruit infusion consumption. International Journal of Food Sciences and Nutrition, 65(6), 740-744. [CrossRef]
29. Topuzović, M.D., Stanković, M.S., Jakovljević, D.Z., Bojović, B.M. (2016). Plant part variability of Sambucus ebulus L. secondary metabolites content and antioxidant activity. Agro FOOD Industry Hi Tech, 27(2), 60-63.
30. Barak, T.H., Celep, E., ˙Inan, Y., Yeşilada, E. (2020). In vitro human digestion simulation of the bioavailability and antioxidant activity of phenolics from Sambucus ebulus L. fruit extracts. Food Bioscience, 37, 100711. [CrossRef]
31. Nascimento, P.G.G. do, Lemos, T.L.G, Bizerra, A.M.C., Arriaga, Â.M.C., Ferreira, D.A., Santiago, G.M.P., Braz-Filho, R., Costa, J.G.M. (2014). Antibacterial and antioxidant activities of ursolic acid and derivatives, Molecules, 19(1), 1317-1327. [CrossRef]
32. Anton, A.M., Pintea, A.M., Rugină, D.O., Sconţa, Z.M., Hanganu, D., Vlase, L., Benedec, D. (2013). Preliminary studies on the chemical characterization and antioxidant capacity of polyphenols from Sambucus sp.. Digest Journal of Nanomaterials and Biostructures, 8(3), 973 – 980.
33. Meriç, Z.İ., Bitiş, L., Birteksöz-Tan, S., Özbaş Turan, S., Akbuga, J. (2014). Antioxidant, antimicrobial and anticarcinogenic activities of Sambucus ebulus L. flowers, fruits and leaves. Marmara Pharmaceutical Journal, 18(1), 22-25. [CrossRef]
34. Zahmanov, G., Alipieva, K., Denev, P., Todorov, D., Hinkov, A.., Shishkov, S., Simova, S., Georgiev, M.I. (2015). Flavonoid glycosides profiling in dwarf elder fruits (Sambucus ebulus L.) and evaluation of their antioxidantand anti-herpes simplex activities. Industrial Crops and Products, 6(3), 58–64. [CrossRef]
35. Rodino, S., Butu, A., Petrache, P., Butu, M., Dinu-Pîrvu, C., Cornea, C.P. (2015). Evaluation of the antimicrobial and antioxidant activity of Sambucus ebulus extract. Farmacia, 63(5), 751-754.
36. Motevalli-Haghi, S.F., Fathi, H., Ebrahimzadeh, M.A., Eslami, S., Karamie, M., Eslamifar, M., Dehghan, O. (2020). Evaluation of phytochemical, total phenolic and flavonoid content, antioxidant activities, and repelling property of Sambucus ebulus. Journal of Medicinal Plants and By-products, 9, 97-105.
37. Sidor, A., Gramza-Michałowska, A. (2015). Advanced research on the antioxidant and health benefit of elderberry (Sambucus nigra) in food – a review. Journal of functional foods, 18(B), 941–958. [CrossRef]
38. Azari, B., Siami, A., Ebrahimzadeh, M.A., Khan, B.A. (2015). Antıoxidants activity of extracts from Sambucus nigra, efficiency of different extraction methods. Latin American Applied Research, 45(3), 139-144. [CrossRef]
39. Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., Rupasinghe, H.P. (2018). In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines, 6(4), 107. [CrossRef]
40. Shinde, U.A., Phadke, A.S., Nair, A.M., Mungantiwar, A.A., Dikshit, V.J., Saraf, M.N. (1999). Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. [CrossRef]
41. Süntar, İ.P., Kupeli-Akkola, E., Yalçin, F.N., Koca, U., Keleş, H., Yeşilada, E. (2010). Wound healing potential of Sambucus ebulus L. leaves and isolation of an active component, quercetin 3-O-glucoside. Journal of Ethnopharmacology, 129(1), 106–114. [CrossRef]