GC / MS metodu ile idrarda aynı anda morfin , kodein ve 6-monoasetilmorfin tayini

Bu çalışmada idrarda morfin, kodein ve 6-monoasetilmorfin (6-MAM)' nin aynı anda tespit edilebilmesine yönelik bir yöntem kurulmuştur. Bu yöntemde tüketme, katı faz tüketimi (Solid Phase Extraction) kolonlarında yapılmış ve N,O,bis (trimetilsilil) trifloroasetamid (BSTFA) ile %1 trimetilklorosilan (TMCS) kullanılarak türevlendirme yapılmıştır. Ölçümler kütle dedektörlü gaz kromatografisinde (GC/MS), SİM modu (seçici iyon modu) kullanılarak yapılmış ve kullandığımız opiatların her üçü içinde % 90 nın üzerinde verim (geri kazanılabilirlik) değerleri elde edilmiştir. Çalışmamızda ayrıca morfin kullanan bir şahsın idrarında da kurmuş olduğumuz bu yöntem uygulanmıştır.

Simultaneous determination of morphine, codeine and 6-monoacetylmorphine in urine by GC / MS method

In the present study a method was developed for simultaneous determination of morphine, codeine and 6-MAM (6-monoacetylmorphine) in urine. We used SPE (Solid Phase Extraction) method in combination with the BSTFA + 1 % TMCS derivatization method. Morphine, codeine and 6-MAM were determined in urine by using GC/MS method. We determined recovery rates over 90 % for the opiates mentioned above. This method was also applied for the urine obtained from a morphine addict.

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