Şükrü KİRKAN, Göksel ERBAŞ, Serap SAVAŞAN, Neşe UÇAN, Saadet GÜRPİNAR, Volkan ÖZAVCİ

Subklinik Mastitisli Saanen Keçilerinden İzole Edilen Staphylococcus aureus İzolatlarının Antimikrobiyel Dirençlerinin Belirlenmesi

Özbilgi/Amaç: Mastitis, bir ya da birden çok mikroorganizma tarafından oluşturulan meme bezinin yangısı ile karakterize bir hastalıktır. Süt içinde bulunan mikroorganizmaların varlığı genellikle mikrobiyel kültür yöntemi ile ortaya konabilmektedir. Staphylococcus aureus, keçilerde en sık teşhis edilen ve meme içi infeksiyonlara yol açan mikroorganizmadır. Bu çalışmanın amacı, Saanen keçilerinden izole edilen S. aureus etkenlerine karşı antimikrobiyel ajanların dirençliliğini tespit etmektir.Materyal ve Metot: Süt örnekleri, Batı Türkiye bölgesinde bulunan bir Saanen keçisi çiftliğinden toplanmıştır. Erken laktasyon periyodunda bulunan her bir hayvanın iki meme lobundan olmak üzere 280 adet 140 Saanen keçisi süt numunesi tek sefer örnekleme ile toplanmıştır. Toplanan süt numuneleri, % 5 koyun kanlı agara ekilmiş ve 37oC’de 24-48 saat inkube edilmiştir. Daha sonra, bütün izolatlar için biyokimyasal testler ve API ID32 STAPH sistemi bioM´erieux® kullanılmış ve izolatlar S. aureus olarak identifiye edilmiştir.Bulgular ve Sonuç: Bu çalışmada subklinik mastitisli Saanen keçilerinden alınan 280 adet süt numunesinden 138 % 49.3 adet bakteriyel izolasyon yapılmıştır. Bakteriyel izolatlardan 90 % 32.1 adedi S .aureus olarak identifiye edilmiştir. Bütün S. aureus n=90 suşları antimikrobiyel duyarlılık testine tabi tutulmuştur. S. aureus izolatları, oksitetrasikline karşı % 100, ampisiline karşı % 91 ve vankomisine karşı % 60 oranında dirençlilik göstermiştir. S. aureus etkeni, subklinik mastitiste etiyolojik ajan olarak sayıca daha fazla bulunmuştur ve novobiosin, Saanen keçilerinde görülen subklinik mastitis hastalığında söz konusu etkene karşı en etkili antibiyotik olarak tespit edilmiştir.

Anahtar Kelimeler:

Saanen Keçisi, Subklinik Mastitis, S. aureus, İdentifikasyon, Antimikrobiyel dirençlilik

Detection of Antimicrobial Resistance in Staphylococcus aureus Isolated from Saanen Goats with Subclinical Mastitis

Background/Aim: The aim of the present study is to describe the distribution of viral antigen localization in nonnervous tissues was described in goats naturally infected with caprine arthritis encephalitis virus.Materials and Methods: The milk samples were collected from one Saanen goats flock in the Western Turkey. A total of 280 half-udder milk samples 140 Saanen goats were collected in a single sampling time in a flock during the early lactation period. The milk samples were incolated to 5 % sheep blood agar incubated at 37oC for 24-48 hours. After that, all isolates were identified as S. aureus, using biochemical tests and using API ID32 STAPH system bioM´erieux® . Results and Conclusion: In this study, bacterial isolation was made from 138 49.3% out of 280 milk samples taken from subclinical mastitic milk of Saanen goat. Bacterial isolates were identified as S. aureus 90 32.1% . All S. aureus isolates n=90 were subjected to antimicrobial susceptibility testing. S. aureus isolates were resistant to oxytetracycline 100%, ampicillin 91% and vancomycin 60%. S. aureus are more in number as causative agents in subclinical mastitis and novobiocin is the most effective antibiotic in Saanen subclinical mastitis.

Keywords:

Saanen Goat, Subclinical Mastitis, S. aureus, Identification, Antimicrobial resistance.,

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