Ruminantlarda Border Hastalığı Üzerine Bir Derleme: Moleküler Tiplendirme, Patogenezis, Tanı ve Kontrol

Bu derleme; küçük ruminant sürülerinde abortlar, kongenital anomalili yavrular ve yaşama gücü zayıf yavruların doğumu ile karakterize olan ve pestivirusların neden olduğu Border Hastalığı BD hakkında son yıllarda gerçekleştirilen virolojik tanı, etken karakterizasyonu, patogenezis ve enfeksiyonun kontrolüne yönelik çalışmaları içermektedir. Sığır virus diare virus ve klasik domuz vebası virusu ile çok yakınlığı olan BD virus BDV , Flaviviridae ailesinin Pestivirus genusunda yer alır. 5’-NTR ve Npro gen bölgesini hedef alan filogenetik analizler koyun pestiviruslarını en az 6 alt grupta sınıflandırmaktadır. Türkiye küçük ruminantlardan elde edilen izolatların filogenetik analizlerinin sonuçları; BDV’ların, bilinen BDV-3 subgenomundan farklı olarak, BDV-3 ve BDV-7 olduğunu ortaya koymuştur. Her iki izolat genetik olarak aynı genustan klasik domuz vebası ile çok yakın ilişkili bulunmuştur. BD atıklar, konjenital anomaliler, yaşama gücü zayıf ve persiste enfekte zayıf yavru doğumları ile karakterizedir. Nekropsi muayenesinde, atık ve neonatal küçük ruminantlarda porensefali, hidranensefali, hidrosefalus ve serebellar hipoplazi en çok dikkati çeken bulgulardır. Makroskopik ve histopatolojik değişiklikler genellikle mavidil, akabane ve enzootik ataksi ile karışır. Ayırıcı tanı için, virolojik ve patolojik incelemelerde pestivirus antijen ve genomunu belirlemek gerekir. BD küçük ruminantlarda dünya genelinde yaygın bir hastalıktır ve önemli ekonomik kayıplara neden olur. Ayrıca, enfekte küçük ruminantlar, türler arası nakil nedeniyle, ruminant populasyonları arasında pestivirus enfeksiyonlarının yayılımında anahtar bir rol oynarlar. Pestivirus kontrol programlarında bu rezervuarların büyük önemi unutulmamalıdır.

A Review of Border Disease Virus Infection in Ruminants: Molecular Characterization, Pathogenesis, Diagnosis and Control

This review includes the recent studies of virological diagnosis, agent characterization, pathogenesis, and control of Border Disease BD infection caused by viruses in the genus Pestivirus. BD virus BDV , closely related to Bovine Viral Diarrhea Virus and Classical Swine Fever Virus, is classified in the genus Pestivirus of the family Flaviviridae. Based on phylogenetic analysis, ovine pestiviruses are segregated at least into six clusters, using the 5’-NTR and Npro gene region sequences. Phylogenetic analysis of BDV isolated from Turkey isolates reveal that the virus form a distinct cluster as BDV-7 and BDV-3, which isolates are being more closely related to CSFVs than to the known BDV-3 subgenotype that contains pig-derived Pestivirus obtained from sheep. BD infection is characterized by abortions, congenital abnormalities, and stillbirths, the birth of small weak lambs and persistent infections of the offspring in small ruminant flocks. On postmortem examination, porencephaly, hydranencephaly, hydrocephalus, and cerebellar hypoplasia are most marked lesions in aborted fetuses and neonatal animals. The gross and histologic lesions are generally consistent with bluetongue, akabane, and enzootic ataxia of small ruminants. Therefore, labeling the Pestivirus viral antigen and genome is essential to clarify the differential diagnosis. BD is reported worldwide and leads to significant losses in the ruminant population. In addition, the infected small ruminants play a key role in the spread of Pestivirus infections among ruminant population by interspecies transmission. The great importance of these reservoirs should not be forgotten in Pestivirus control programs.

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