Eva Aisser Botres AJAJ, Maab. I. Al- FARWACHİ

Irak’ ta Nineveh Bölgesindeki Sığırlarda Leptospira interrogans hardjo ve pomona Serotiplerinin Serosürveyansı

Bu çalışmada Irak’ta Nineveh bölgesindeki sığırlarda anti-Leptospira hardjo ve anti-L. pomona antikorlarının belirlenmesi amaçlandı. Bu amaçla 7 aylık bir süreçte, Eylül 2012 ile Mart 2013 arasında, 2 yaş ve üzeri 96 yerli sığır ırkında serum örnekleri toplandı. Leptospira interrogans hardjo ve pomona serotipleri ticari olarak elde edilen ELİZA test kitleri ile belirlendi. Doksanaltı serum örneğinin 6’sı % 6.32 Leptospira hardjo yönünden, yalnızca 1’i %1 L. pomona yönünden pozitifti. Bu bulgular leptospirozis’in bu bölgedeki sığırlarda mevcut olduğunu göstermektedir.

Anahtar Kelimeler:

ELISA, Leptospirozis, Leptospira hardjo, Serolojik tanı, Sığır

Serosurvey of Leptospira interrogans Serovars hardjo and pomona in Cattle in Nineveh Province, Iraq

The purpose of the present study was to determine the serodiagnosis of anti-Leptospira hardjo and anti-L. pomona antibodies in cattle in Nineveh province, Iraq. For this purpose 96 sera were collected from adult native breed cattle 2 years old or over during an 7 month period from September 2012 to March 2013. Serodiagnosis of Leptospira interrogans serovars hardjo and pomona was carried out using a commercial ELISA. From a total of 96 sera, 6 6.3% were positive for Leptospira hardjo and only one animal 1 % was seropositive to L. pomona. These data suggest that leptospirosis is present among cattle in the study area.

Keywords:

Leptospirosis , Cattle , ELISA , Serodiagnosis , Leptospira hardjo.,

PDF

___

Al-Badrawi TYG, Habasha FG , Sultan SH ( 2010). Serological study of Leptospirosis in cattle, sheep and goats in Baghdad Province . Al- Anbar Journal Veterinary Science , 3,78-82.
Adler B, Moctezuma A (2010). Leptospira and leptospirosis. Veteri- nary Microbiology, 140, 287–296.
Bahaman AR , IBRAHIM AL , ADAM H (1987). Serological prevalence of leptospiral infection in domestic animals in West Malaysia. Epi- demiology and Infection , 99, 379-392
Bolin CA. ( 2003). Leptospirosis. In: Fowler ME, Miller RE. Zoo and wild animal medicine. St. Louis, Missouri: WB Saunders , 699–702.
Cole JR , Sulzer CR , Pursell AR ( 1973). Improved microtechnique for the leptospiral microscopic agglutination test. Applied Microbiolo- gy, 25, 976–980.
Ezeh AO , Addo PB , Adesiyun AA , Bello CSS , Makinde A ( 1989). Serological prevalence of bovine leptospirosis in Plateau State, Ni- geria. Revue D’élevage et de Médecine Vétérinaire Des Pays Tropi- caux , 42,505-508.
Dassanayake DLB ,Wimalaratna H , Agampodi SB , Liyanapathrirana VC , Piyarathna TACL , Goonapienuwala BL ( 2009). Evaluation of surveillance case definition in the diagnosis of leptospirosis, using the Microscopic Agglutination Test: a validation study. BMC Infec- tious Diseases, 9:48.
El Jalii IM ( 2008). Comparison between ELISA and the Microscopic Agglutination a test for the Diagnosis of Bovine Leptospirosis Re- vue D’élevage et de Médecine Vétérinaire Des Pays Tropicaux , 61 (2) , 73-75.
Faine S , Adler B , Bolin C , Perolat P (2000). Leptospira and Leptospi- rosis, 2nd Ed., Med. Sci. , Melbourne, Australia.
Kavanagh OV , Skibinska A , Mackie DP, Montgomery JM, Logan EF, Ellis WA ( 2002). In Bovine leptospirosis: Validation of an ELISA to detect antibodies to Leptospira borgpetersenii serovar hardjo. Re- search in Veterinary Science, 72:26-26
Kocabiyik A L, Cetin C (2004 ). Bovine leptospirosis in south Marmara region of Turkey : A serological survey. Revue de Médecine Vétéri- naire,155, 12, 606-608
Larson RL , Maas J , Richey E , Bolin, C ( 2007). Herd Prevalence and Risk Factors of Leptospira Infection in Beef Cow/calf Operations in the United States: Leptospira borgpetwerseni Serovar Hardjo. Bo- vine Practitioner,41, 15-23.
Leonard N , Mee JF , Snijders S, Mackie D (2004). Prevalence of an- tibodies to Leptospira interrogans serovar hardjo in bulk tank milk from unvaccinated Irish dairy herds. Irish Veterinary Journal , 57, 226-231.
Miller DA , Wilson MA , Beran GW (1991). Survey to estimate prev- alence of Leptospira interrogans infection in mature cattle in the United States. American Journal of Veterinary Research , 52,1761– 1765.
Machang URS, Mgdde G, Mpanduji D (1997 ). Leptospirosis in animals and humans in selected areas of Tanzania. Belgian Journal of Zool- ogy , 127, 97-104.
Mariyar C P , Kumar AA, Thangapandian E , Amuthar R, Srivastavava SK ( 2006). Evaluation of a recombinant LipL41 antigen of Leptospi- ra interrogans serovar canicola in ELISA for
serodiagnosis of bovine leptospirosis. Comparative Immunology, Mi- crobiology & Infectious Diseases., 29,269-277.
Morgan LH , Mark AW , Tammy MA , Corrie LT , David AM (2007). Comparative serological study of Leptospira serovar hardjo geno- types for use in the microscopic agglutination test Journal of Veter- inary Diagnostic Investigation,19, 84–87 .
Peregrine AS, Martin SW, Hopwood DA (2006). Neospora caninum and Leptospira serovar serostatus in dairy cattle in Ontario. Canadi- an Veterinary Journal ,47, 467–470.
Pritchard DG (1986 ). National situation of leptospirosis in the United Kingdom. In: W.A. ELLIS and T.W.A. LITTLE (ed.) Present state of lep- tospirosis diagnosis and control. Martinus Nijhoff, Dordrecht, The Netherlands ,pp 221-233
Radostits OM, Gay CC, Hinchcliff KW, Constable PD. (2007). In: Veter- inary Medicine: A Textbook of the Diseases of Cattle, Horses,sheep, Pigs and Goats. Saunders (Elsevier), Edinburgh, pp. 1094-1110.
Rochat T (1998) .A review of leptospirosis in farm animals in Portugal. Revue scientifique et technique , 17, 699-712.
Ryan EG , Leonard N, Luke OG, Michael LD , Simon JM (2012). Herd-level risk factors associated with Leptospira Hardjo seroprev- alence in Beef/Suckler herds inthe Republic of Ireland. Irish Veter- inary Journal ,65, 6
Surujballi OM , Mallory M (2004 ) .An indirect enzyme linked immu- nosorbent assay for the detection of bovine antibodies to multiple Leptospira serovars .The Canadian Journal of Veterinary Research ,68,1–6.
Thiermann AB (1984). Leptospirosis: current developments and trends. Journal of the American Veterinary Medical Association , 184, 722-725.
Victoriano AF, Smythe LD , Gloriani-Barzaga N, Cavinta LL , Kasai T , Limpakarnjanarat K , Ong BL, Gongal G, Hall J , Coulombe CA , Yanagihara Y, Yoshida S, Adler B (2009). Leptospirosis in the Asia Pacific region. BMC Infectious Diseases , 9,147.
Wagenaar J , Zuerner RL, Alt D, Bolin CA. ( 2000). Comparison of polymerase chain reaction assays with bacteriologic culture, immu- noﬂuorescence, and nucleic acid hybridization for detection of Lep- tospira borgpetersenii serovar hardjo in urine of cattle. American Journal of Veterinary Research , 61, 316–320.
World Health Organization. ( 2003). Human Leptospirosis: Guidance for Diagnosis Surveillance and Control. Geneva: World Health Organi- zation,:109, ISBN 92-4-154589-5.