Chenopodium botrys L. ekstraktlarının fenolik taraması ve biyolojik aktiviteleri

Chenopodium cinsi, 200'den fazla türe sahip ve hemen hemen tüm Dünya’da yayılış gösteren tek yıllık veya çok yıllık otsu çiçekli bitki türlerini içeren bir cinstir. Chenopodium cinsine ait türler çeşitli hastalıkların tedavisinde kullanılırken, modern farmasötik araştırmalar güçlü antikanser, antifungal ve antibakteriyel potansiyellerini ortaya çıkarmıştır. Bu çalışmada, Chenopodium botrys (metanol ve etanol) ekstraktlarının bazı biyolojik etkileri (antioksidan ve sitotoksik potansiyelleri), çeşitli analitik yaklaşımlar yardımıyla değerlendirilmiştir. Çalışmada ekstraktlarda bulunan 4 fenolik bileşenin tespiti HPLC tekniği ile yapılmış, bitkinin antioksidan aktivitesini belirlemek için TPC, RPA ve DPPH yöntemleri kullanılmıştır. Ekstraktların HL60 hücre hattı üzerindeki sitotoksik etkisini belirlemek için ise MTT testi kullanılmıştır. Sonuçlar, her iki ekstraktın da değerlendirilmesi gereken önemli özelliklere sahip olduğunu ve özellikle etanolik ekstraktın her yönden (TPC, antioksidan kapasite ve sitotoksisite) metanolik ekstrakttan daha etkili olduğunu göstermiştir. Sonuç olarak, yeni ve etkili fitokimyasalların izolasyonu ve karakterizasyonu için bu bitkinin daha fazla araştırılması gerektiği açıktır. Bu çalışmanın gelecekteki çalışmalar için bir öncü olabileceğini umuyoruz.

Phenolic screening and biological activities of Chenopodium botrys L. extracts

The genus Chenopodium is a genus that includes over 200 species and contains annual or perennial herbaceous flowering plant species scattered almost worldwide. While species belonging to the Chenopodium genus are used to treat various diseases, modern pharmaceutical research has revealed their strong anticancer, antifungal and antibacterial potential. In this study, some biological effects of Chenopodium botrys (methanol and ethanol) extracts were evaluated by helping some analytical approaches. The antioxidant and cytotoxic potentials of the extracts were revealed. In this study, 4 phenolic components in the extracts were determined with the HPLC technique and to determine the antioxidant activity of the plant extracts TPC (total phenolic content), RPA (reducing power activity), and DPPH (2,2′-diphenyl-1-picrylhydrazyl) methods were used. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effect of the extracts on the HL60 cell line. The results showed that both extracts have important properties that should be evaluated, and especially the ethanolic extract is more effective than the methanolic extract in all aspects (TPC, antioxidant capacity, and cytotoxicity). As a conclusion, it is clear that this plant needs to be investigated further for the isolation and characterization of new and effective phytochemicals. We hope that this study could be a precursor for future studies.

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