Gökhan KUŞ, Mete ÖZKURT, Nilüfer ERKASAP, Tülay KÖKEN, Ömer ÇOLAK, Halit Buğra KOCA, Selda KABADERE, Oğuzhan KOCA

Acorus calamus bitki ekstraktının prostat kanser hücre kültürü üzerine etkileri

Batılı ülkelerde erkekler arasında prostat kanseri en sık tanısı koulan kanser türüdür ve erkeklerde kanser nedeniyle ölümde ikinci sırada yer almaktadır. Prostat kanserinde vasküler endotelyal büyüme faktör-A’nın (VEGF-A) tek başına en önemli anjiogenik faktör olduğu düşünülmektedir. Apoptotik süreçte yer alan poli-(ADP-riboz) polimeraz’ın (PARP) yıkılımı apoptotik süreç markırı olarak kullanılmaktadır. Acorus calamus’un anti-kanserojen etkisi ile birlikte çeşitli tıbbi özelliklere sahip olduğu uzun yıllardır özellikle Asya’da kabul edilmektedir. Çalışmamızda A. calamus kökünün etanolik ekstraktının insan prostat kanser LNCaP hücre dizisinde hücre çoğalmasına, anjiogeneze ve apoptozise olası etkileri in vitro ortamda çalışıldı. Hücreler 24 ve 48 saat boyunca çeşitli ekstrakt konsantrasyonlarıyla muamele edildi. Bu konsantrasyonlardaki ekstraktlar 24 ve 48 saatte LNCaP hücre yaşayabilirliğini kontrolle karşılaştırıldığında doza ve zamana bağlı olarak %44’lere kadar azalttı. Bölünmüş PARP, VEGF-A proteini ve gen ekspresyon miktarlarında 24 ve 48 saat sonra belirgin değişiklikler gözlemlendi. Bu çalışma A.calamus kökünün etanolik ekstraktının zaman ve doz bağımlı olarak güçlü bir anti-kanser, anti-anjiogenik ve apoptotik etkileri olabileceğini ortaya koymaktadır.

Effects of Acorus calamus plant extract on prostate cancer cell culture

In western countries,prostate cancer is the most frequently diagnosed cancer and the second mostcommon cause of death from cancer in men. Vascular endothelial growth factor-A(VEGF-A), thought to be the single most important angiogenic factor in prostatcancer. Poly-(ADP-ribose) polymerase (PARP) involved in apoptotic process andcleavage of PARP serves as a marker of cells undergoing apoptosis. Acorus calamus have long been consideredto have anti-carcinogenic and medicinal properties especially in Asia. Weexamined whether ethanolic extract of A.calamus root affects the survival of prostate cancer LNCaP cells andinduces apoptosis and angiogenesis of these cells in vitro. Cells were incubated during 24 and 48 hours with variousdoses of extract. Extract with these concentrations reduced the number of LNCaPliving cells up to 44 % as compared to the control at dose and time dependentmanner at 24 and 48 hours. Significantly alterations were observed at cleavedPARP, VEGF-A protein and gene expression amounts after 24 and 48 hours. Thepresent study reveals the possibility that ethanolic extract of A. calamus root posseses a dose and timedependent anticancer, apoptotic and anti-angiogenic properties.

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