Atakan BENEK, Kerem CANLI, Ergin Murat ALTUNER

Bazı Briyofitlerin Antimikrobiyal ve Antioksidan Aktiviteleri

Bu çalışmada, Plagiomnium medium (Bruch & Schimp.), Leptodon smithii (Hedw.), Rhynchostegium alopecuroides(Brid.) A.J.E.Sm., Nogopterium gracile (Hedw.) Crosby & W.R.Buck, Pylasia polyantha (Hedw.) ve Timmia bavarica (Hessl.)’nın in vitro antimikrobiyal ve antioksidan aktiviteleri araştırılmıştır. Hazırlanan karayosunu etanol ekstraktlarının antimikrobiyal aktivitesi 20 suşa karşı disk difüzyon yöntemi kullanılarak belirlenmiştir. Antioksidan aktiviteyi belirlemek için DPPH (2,2-difenil-1-pikrilhidrazil) yöntemi kullanılmıştır. Antimikrobiyal aktivite testi sonucunda önemli bir hastane enfeksiyonu olan çoklu ilaca dirençli ve standart Staphylococcus aureus suşlarına karşı etki görülmüştür. Antioksidan aktivite testi sonucunda tüm örneklerde antioksidan etki gözlenmiş ve 6,0826 μg/mL EC50 değeri ile P. medium örneğinin en yüksek etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler:

Antimikrobiyal aktivite, Antioksidan aktivite, Disk difüzyon yöntemi, DPPH, Karayosunu

Antimicrobial and Antioxidant Activities of Some Mosses

In this study, in vitro antimicrobial and antioxidant activity of Plagiomnium medium (Bruch & Schimp.), Leptodon smithii (Hedw.), Rhynchostegium alopecuroides(Brid.) A.J.E.Sm., Nogopterium gracile (Hedw.) Crosby & W.R.Buck, Pylasia polyantha (Hedw.) and Timmia bavarica (Hessl.) was investigated. The antimicrobial activity of prepared moss ethanol extracts was determined using the disk diffusion method against 20 strains. The DPPH (2,2-difenil-1-pikrilhidrazil) method was used to determine the antioxidant activity. As a result of the antimicrobial activity test, it was found to be effective against multi-drug resistant and standard Staphylococcus aureus strains, which is an important hospital infection. As a result of the antioxidant activity test, an antioxidant effect was observed in all samples, and it was determined that the P. medium sample had the highest effect with an EC50 value of 6.0826 μg/mL.

Keywords:

Antimicrobial activity, Antioxidant activity, Disk diffusion method, DPPH, Moss,

PDF

___

Altuner E.M. Canli K. Akata I. 2014. Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme. Journal of Pure and Applied Microbiology. 8:1, 539-545.
Andrews J.M. 2007. BSAC standardized disc susceptibility testing method (version 6). Journal of Antimicrobial Chemotherapy. 60:1, 20-41.
Asakawa Y. Ludwiczuk A. Nagashima F. 2013. Chemical constituents of bryophyta. Chemical Constituents of Bryophytes. Progress in the Chemistry of Organic Natural Products Vol. 95 Vienna: Springer. 563-605.
Benek A. Şenturan M. Şimşek Ö. Canlı K. Altuner E.M. 2021. Bazı Karayosunu ve Ciğerotu Türlerinin Antimikrobiyal Aktivitesinin Belirlenmesi. Anatolian Bryology. 7:1, 1-7.
Bozyel M.E. Şenturan M. Benek A. Merdamert Bozyel E. Canli K. Altuner E.M. 2019. In vitro antimicrobial activity screening of Heliotropium europaeum against wide range of microorganisms and multi drug resistant (MDR) bacteria. European Journal of Biomedical and Pharmaceutical Sciences. 6:3, 113-117.
Canli K. Altuner E.M. Akata I. 2015. Antimicrobial screening of Mnium stellare. Bangladesh Journal of Pharmacology. 10:2, 321-25.
Carranza M.S.S. Linis V.C. Ragasa C.Y. Tan M.C.S. 2019. Chemical constituents and antioxidant potentials of seven Philippine mosses. Malaysian Journal of Analytical Sciences. 23:6, 950-962.
Chen F. Ludwiczuk A. Wei G. Chen X. Crandall-Stotler B. Bowman J.L. 2018. Terpenoid secondary metabolites in bryophytes: chemical diversity, biosynthesis and biological functions. Critical Reviews in Plant Sciences. 37:2-3, 210-231.
Core R Team. 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ [Erişim tarihi: 15/12/2017].
Ertürk Ö. Sahin H. Ertürk E.Y. Hotaman H.E. Koz B. Özdemir Ö. 2015. The antimicrobial and antioxidant activities of extracts obtained from some moss species in Turkey. Herba Polonica. 61:4, 52-65.
Ilhan S. Savaroğlu F. Çolak F. İşçen, C.F. Erdemgil F.Z. 2006. Antimicrobial activity of Palustriella commutata (Hedw.) ochyra extracts (Bryophyta). Turkish Journal of Biology. 30:3, 149-152.
Krzaczkowski L. Wright M. Rebérioux D. Massiot G. Etiévant C. Gairin J.E. 2009. Pharmacological screening of bryophyte extracts that inhibit growth and induce abnormal phenotypes in human HeLa cancer cells. Fundamental & clinical pharmacology. 23:4, 473-482.
Kürschner H. Frey W. 2020. Bryophyte novelties from the Arabian Peninsula and Socotra Island. Nova Hedwigia. 281-286.
Mensor L.L. Menezes F.S. Leitão G.G. Reis A.S. Santos T.C.D. Coube C.S. Leitão S.G. 2001. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research. 15:2, 127-130.
Onbasli D. Yuvali G. 2021. In vitro medicinal potentials of Bryum capillare, a moss sample, from Turkey. Saudi Journal of Biological Sciences. 28:1, 478-483.
Özen-Öztürk Ö. Özdemir T. Batan N. Erata H. 2023. Three Sphagnum taxa new to Turkey and South-West Asia. Botanica Serbica. 47:1, 47-53.
Turu D. Bozyel M.E. Candan K. Yakan M.A. Benek A. Canli K. 2020. In vitro antimicrobial and antioxidant activities of Pyracantha coccinea fruits ethanol extract. Vitro. 4: 89-93.
Uyar G. Doğru N.H. Ören M. Çavuş A. 2016. Determining Antibacterial Activity of Some Mosses (Cinclidotus riparius (Host ex Brid.) Arn., Calliergonella cuspidata (Hedw.) Loeske, Thamnobryum alopecurum (Hedw.) Gangulee, Leucobryum juniperoideum (Brid.) Müll. Hal., Cirriphyllum crassinervium (Taylor) Loeske & M. Fleisch.). Anatolian Bryology. 2:1-2, 1-8.
Vollár M. Gyovai A. Szűcs P. Zupkó I. Marschall M. Csupor-Löffler B. Urbán E. 2018. Antiproliferative and antimicrobial activities of selected bryophytes. Molecules. 23:7, 1520.
Whitehead J. Wittemann M. Cronberg N. 2018. Allelopathy in bryophytes-a review. Lindbergia. 41:1.
Yayintas O. T. Ozyurt D. Demirata B. Yuceer Y.K. Yilmaz S. 2019. Chemical Composition and Antioxidant Capacities of Mosses Gathered from Mount Ida (Canakkale, Turkey). Fresenius Environmental Bulletin. 2413.