Turunçgil arastırmalarında biyoteknoloji çalısmaları
Tarımsal alanda abiyotik ve biyotik stresler ve yeni çesitlere olan taleplerin artması daha etkili araçların gelistirilmesini zorunlu kılmaktadır. Bu konu ile ilgili olarak tarımsal arastırmalar alanında biyoteknolojinin kullanımı son dönemlerde hızla artmıs ve bu alana önemli katkılar sunmustur. Turunçgil arastırmalarında biyoteknoloji yaygın olarak taksonomik çalısmalar, hastalıktan arındırılmıs ve hızlı bitki materyali üretimi, genetik çesitliliğin tespiti, genetik haritalama, transformasyon ve gen kaynaklarının etkili korunması amacıyla kullanılmaktadır. Bu çalısmada söz konusu konularda yapılan çalısmalar gözden geçirilmekte ve önemli konular vurgulanmaktadır. Ana baslıklar üretim ve ıslah, taksonomi, gen kaynaklarının korunması ve değerlendirilmesi ile sonuç bölümlerinden olusmaktadır.
Biotechnological studies in citrus research
Abiotic and biotic stress and demand to new cultivars force to develop more efficient tools in agricultural industry. Related to these topics, the use of biotechnology in agricultural research have made significant contribution. Biotechnology is commonly used in Citrus research for taxonomic studies, rapid production of disease-free plant material, detection of genetic diversity, genome mapping, transformation and germplasm resources. As a result, biotechnology brought great opportunities to improve citrus fruits. In this review, the results of studies done in this area were reviewed and critical issues were emphasized. Main titles are production and breeding, conservation and utilization of genetic resources, and conclusion.
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- Abbas, M., Khan, M.M., Mughal, S.M., Jaskani, M.J., Abbas, H., 2006. Propagation of ctv-free sweet orange [Citrus sinensis (l.) Osbeck] plants through microbuddıng technique. Pak. J. Bot., 38: 583-587.
- Ali, S., Mirza, B., 2006. Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explant type and hormone concentration. Acta Bot. Croat., 65:137–146.
- Almeida, W.A.B., Filho, F.A.A.M., Mendes, B.M.J., Rodriguez, A.P.M., 2002. In vıtro organogenesıs optımızatıon and plantlet regeneratıon ın Citrus sinensis nd C. limonia. Sci. Agric., 59:35-40.
- Ananthakrishnan, G., Orbovic, V., Pasquali, G., Calovic, M., Grosser, J.W., Newton, R.J., 2007. Transfer of citrus tristeza virus (CTV)-derived resistance candidate sequences to four grapefruit cultivars through Agrobacterium-mediated genetic transformation. In vitro Cell & Dev. Biology-Plant, 43:593-601.
- Barkley, N.A., Roose, M.L., Krueger, R.R., Federici, C.T., 2006. Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor. Appl. Genet., 112: 1519–1531.
- Barbosa-Mendes, Jm., Filho, F.A.A.M., Filho, A.B., Harakava, R., Beer, S.V., Mendes, B.M.J., 2009. Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker. Sci. Hort., 122:109-115.
- Barlass, M., Skene, G.M., 1986. Citrus (Citrus species). P. 207-219. In: Y.P.S. Bajaj (eds.). Biotechnology in agriculture and forestry. Vol. 1: trees I. Springer.Berlin.
- Barrett, H.C., Rhodes, A.M., 1976. A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Sys. Bot. 1:105-136.
- Baruah, A., Nagaraju, V., Parthasaraty, V.A., 1996. Micropropagation of three endangered Citrus species. 1. Shoot proliferation in vitro. Annals Plant Physiology 10:124-128.
- Ben-Hayyim, G., Goffer, Y., 1989. Plantlet regeneration from a NaCl-selected salt-tolerant callus culture of Shamouti orange (Citrus sinensis L. Osb.). Plant Cell. Rep., 7: 680-683.
- Canel, C., Bailey-Serres, J.N., Roose, M.L., 1996. Molecular characterization of the mitochondrial citrate synthase gene of an acidless pummelo (Citrus maxima). Plant Molecular Biology, 31: 143-147.
- Cepeda-Nieto, A.C., Barera-Saldana, H.A., 1997. Cloning and sequencing of the coat protein gene of a new isolate of citrus tristeza virus from Mexico. Plant Disease 81: 693.
- Cervera, M., Juárez, J., Navarro, L., Pena, L., 2008. Genetic transformation of mature Citrus plants. Transgenic Plants: Methods and Protocols, 286: 177-187.
- Chaturvedi, H.C., Mitra, G.C., 1975. A shift in morphogenetic pattern in Citrus callus tissue during prolonged culture. Ann. Bot., 39: 683-687.
- Chen-Zhen, G., Jia, F.W., Chen, Z., Wang, J.F., 1995. Study on storage of Citrus germplasm in vitro (brief report). J. Fujian Agricultural Univ., 24: 376.
- Cheng, Y., De Vicente, M.C., Meng, H., Guo, W., Tao, N., Deng, X.X., 2005. A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. Tree Physiology, 25: 661–672.
- Chiancone, B., Tassoni, A., Bagni, N., Germana, M.A., 2006. Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan. J. Plant Biotech., 87:145-153.
- Cipriani, G., Testolin, R., Gardner, R., 1998. Restriction-site variation of PCR-amplified chloroplast DNA regions and its implication for the evolution and taxonomy of Actinidia. Theor. Appl. Genet., 96: 389-396.
- Coelho, S., Da, AF, De, D.L., Siqueira-Bruckner, C.L., De Oliveira, A.B., Cardoso, A.A., Da Silva-Coelho, A.F., De Siqueira, D.L., De Oliveira, A.B., 1998. Plant regeneration from ovule culture of Citrus reticulata cv. Dancy. 33: 29-35.
- Çevik, B., Lee, R.F., Niblett, C.L., 2006. Genetic Transformation of Citrus paradisi with Antisense and Untranslatable RNA-dependent RNA Polymerase Genes of Citrus tristeza closterovirus. Turk. J. Agric. For., 30: 173-182.
- Deka, P.C., Thakur, A.C., 1996. Tissue culture techniques for improvement and conservation of genetic resources. Proc Seminar Problems and Prospects Agric Res Development in North-East India, Assam Agricultural University, Jorhat, India pp 27-28.
- Deng, Z.N., Gentile, A., Nicolosi, E., Vardi, A., Tribulato, E., 1995. Identification of in vivo and in vitro lemon mutants by RAPD markers. J. Hort. Sci., 7: 117-125.
- Deng, Z.N., Shu, H., Shun, Y.X., Gmitter, F.G., Deng, Z.N., Shu, H., Xiao, S.Y., 1997. Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata. Genome 40: 697-704.
- D'Onghia, A.M., Carimi, F., De Pasquale, F., Djelouah, K., Martelli, G.P., 2001. Elimination of Citrus psorosis virus by somatic embryogenesis from stigma and style cultures. Plant Pathol., 50: 266-269.
- Dutt, M., Grosser, J.W., 2009. Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus. Plant Cell, Tissue and Organ Culture, 98: 331- 340.
- Fang, D.Q., Zhang, W.C., Xiao, S.Y., 1993. Studies on taxonomy and evolution of Citrus and its related genera by isozyme analysis (in Chinese with English abstract). Acta Phytotaxon Sin 31: 329-352.
- Fang, D.Q., Federici, C.T., Roose, M.L., 1997. Development of molecular markers linked to a gene controlling fruit acidity in Citrus. Genome, 40: 841-849.
- FAO. 2008. Food and Agricultural Organization of the United Nations, http://faostat.fao.org
- Febres, V.J., Lee, R.F., Moore, G.A., 2008. Transgenic resistance to Citrus tristeza virus in grapefruit. Genetic Transformation and Hybridization, 27:93-104.
- Federici, C.T., Fang, D.Q., Scora, R.W., Roose, M.L., 1998. Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor. App. Genet., 96: 812-822.
- Garcia, A., Asins, A.J., Forner, J., Carbonell, E.A., 1999. Genetic analysis of apomixis in Citrus and Poncirus by molecular markers. Theor. Appl. Genet. 99: 511-518.
- Gauss, C., Kalkum, M., Löwe, M., Lehrach, H., Klose, J., 1999. Analysis of the mouse proteome (I) Brain proteins: Separation by two-dimensional electrophoresis and identification by mass spectrometry and genetic variation. Electrophoresis, 20: 575-600.
- Gmitter, F.G., Ling, X.B., Deng, X.X., 1990. Induction of triploid Citrus plants from endosperm calli in vitro. Theor. Appl. Genet., 80: 785-790.
- Grewal, H.S., Dhatt, A.S., Gosal, S.S., 1994. In vitro shoottip grafting in Citrus. Annals Biology Ludhiana, 10: 1-6.
- Green, R.M., Vardi, A., Galun, E., 1986. The plastome of Citrus, physical map, variation among Citrus cultivars and species and comparison with related genera. Theor. Appl. Genet., 72: 170-177.
- Grosser, J.W., Gmitter Jr., F.G., 1990. Protoplast fusion and citrus improvement, Plant Breed. Rev., 8:339–374.
- Grosser, J.W., Mourao, F.A.A., Gmitter, F.G., Louzada, E.S., Jiang, J., Baergen, K., Quiros, A., Cabasson, C., Schell, J.L., Chandler, J.L., 1996. Allotetraploid hybrids between Citrus and seven related genera produced by somatic hybridization. Theor. Appl. Genet., 92: 577- 582.
- Grosser, J.W., Chandler, J.L., Duncan, L.W., 2007. Production of mandarin + pummelo somatic hybrid citrus rootstocks with potential for improved tolerance/resistance to sting nematode. Sci. Hort., 113: 33-36.
- Guerra, M.S., 1984. Cytogenetics of Rutaceae, II: nuclear DNA content. Caryologia, 37: 219-226.
- Gülsen, O., Roose, M.L., 2001a. Lemons: diversity and relationships with selected Citrus genotypes as measured with nuclear genome markers. J. Amer. Soc. Hort. Sci., 126: 309-317.
- Gülsen, O., Roose, M.L., 2001b. Chloroplast and Nuclear Genome Analysis of the Parentage of Lemons. J. Amer. Soc. Hort. Sci., 126:210-215.
- Gülsen, O., Uzun, A., Canan, I., Seday, U., Canihos, E., 2010. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica 173: 265-277.
- Gutierrez, E.M.A., Luth, D., Moore, G.A., 1997. Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus. Plant Cell. Reports, 16: 745-753.
- Gygi, S.P., Rist, B., Aebersold, R., 2000. Measuring gene expression by quantitative proteome analysis, Curr Opin Biotechnol., 11: 396- 401.
- Herrero, R., Asins, M.J., Carbonell, E.A., Navarro, L., 1996. Genetic diversity in the orange subfamily Aurantioideae. I. Intraspecies and intragenus genetic variability. Theor. Appl. Genet., 92: 599-609.
- Hidaka, T., Omaru, M., Ugaki, M., Tomiyama, M., Kato, A., Oshima, M., Motoyoshi, F., 1990. Agrobacteriummediated transformation and regeneration of Citrus spp. From suspension cells. Jpn. Breed., 40: 199-207.
- Huang, L.C., Chen, W.L., Chiu, D.S., 1988. In vitro graftenhanced nucellar plant development in the monoembryogenic Citrus grandis L. J. Hort. Science, 63: 705-709.
- Jain, S.M., Sopory, S.K., Veilleux, R.E., 1997. In vitro haploid production in higher plants. Volume 5: oil, ornamental and miscellaneous plants. Mohan Jain, S., Sopory, S.K., Veileux, R.E. (eds.) Curr. Plant Science Technology in Agric., 29:256.
- Jarrell, D.C., Roose, M.L., Traugh, S.N., Kupper, R.S., 1992. A genetic map of citrus based on the segregation of isozymes and RFLPs in an intergeneric cross. Theor. Appl. Genet., 84: 49-56.
- Kaneyoshi, J., Kanou, T., Kuwata, Y., Hirao, A., Nakatani, S., Kobayashi, S., 1994. Breeding of triploid citrus cultivars I. Production of triploids from satsuma mandarin (Citrus unshiu Marc.) X tetraploid ponkan mandarin (Citrus reticulata Blanco) crosses. J. Jpn. Soc. Hort. Sci., 66: 9-14.
- Kaneyoshi, J., Kanou, T., Kuwata, Y., Hirao, A., Nakatani, S., Kobayashi, S., 1997. Breeding of triploid citrus cultivars I. Production of triploids from satsuma mandarin (Citrus unshiu Marc.) X tetraploid ponkan mandarin (Citrus reticulata Blanco) crosses. J. Jpn. Soc. Hort. Sci., 66: 9-14.
- Kayim, M., Koc, N.K., Tor, M., 1994. Gene transfer into citrus (Citrus limon L.) nucellar cells by particle bombardment and expression of GUS activity. Turkish J. Agric. Forestry, 20: 349-352.
- Kayim, M., Koc, N.K., 2006. The effects of some carbohydrates on growth and somatic embryogenesis in citrus callus culture. Sci. Hort., 109: 29-34.
- Kepiro, J.L., Roose, M.L., 2010. AFLP markers closely linked to a major gene essential for nucellar embryony (apomixis) in Citrus maxima × Poncirus trifoliata. Tree Genetics and Genomes, 6:1–11.
- Kobayashi, S., Uchimiya, H., 1989. Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer. Jpn. J. Genet., 64: 91-97.
- Komatsu, A., Takanokura, Y., Omura, M. Akihama, T., 1996. Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from a citrus fruit (Citrus unshiu Marc). Mol. Gen. Genet. 252: 346-351.
- Krueger, R., Gülsen, O., Roose, M.L. (Abs). 2000. Use of Molecular Markers in Management of Citrus Germplasm Resources. 9. Int. Soc. Citriculture Meeting, Orlando, USA..
- Liou, P.C., Gmitter, F.G., Moore, G.A., 1996. Characterization of the Citrus genome through analysis of restriction fragment length polymorphisms. Theor. Appl. Genet., 92: 425-435.
- Malik, S.K., Chaudhury, R., 2006. The cryopreservation of embryonic axes of two wild and endangered Citrus species. Plant Genetic Resources, 4: 204-209.
- Mendes, A.F.S., Cidade, L.C., Manzoli, G.N., Otoni, W.C., Filho, W.S.S., Costa, M.G.C., 2008. Tissue culture parameters in sweet orange cultivars. Pesq. Agropec. Bras., 43:1093-1096.
- Moriguchi, T., Hidaka, T., Omura, M., Motomura, T., Akihama, T., 1996. Genotype and parental combination influence efficiency of cybrid induction in Citrus by electrofusion. HortScience, 31: 275-278.
- Mourao-Fo, F.A.A., Gmitter, F.G., Grosser, J.W., 1996. New tetraploid breeding parents for triploid seedless citrus cultivar development. Fruit Varieties Journal, 50: 76-80.
- Muccilli, V., Licciardello, C., Fontanini, D., Patrizia-Russo, M., Cunsolo, V., Saletti, R., 2009. Reforgiato Recupero, G., Foti, S., Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time. J. Proteom., 73: 134- 152.
- Nadel, B., Spiegel-Roy, P., 1987. Selection of Citrus limon cell culture variants resistant to the Mal secco toxin. Plant Sci. 53: 177-182.
- Nicolosi, E., Deng, Z.N., Gentile, A., La Malfa, S., Continella, G., Tribulata, E., 2000. Citrus phylogeny and genetic origin og important species as investigated by molecular markers. Theor. Appl. Genet., 100: 1155- 1166.
- Normah, M.N., Siti Dewi Serimala, M.N., Ellis, R.H., Black, M., Murdoch, A.J. (eds.), Hong, T.D., 1997. Cryopreservation of seeds and embryonic axes of several Citrus species. Basic and applied aspects of seed biology. Proc Fifth International Workshop on Seeds, UK, pp 817-823.
- Oh, S., 1997. The effect of prefreezing treatment and cryoprotectants on the survival of cryopreserved somatic embryos and plant regeneration in Korean native citrus species. Altman, A., Ziv, M., (eds.). 3th Int. Symp. on In vitro Culture and Hortic. Breed., Jerusalem, Israel. Acta Horticulturae, 447: 499-505.
- Ohgawara, T., Kobayashi, S., Ohgawara, E., Uchimiya, H., Ishii, S., 1985. Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor. Appl. Genet., 71: 1-4.
- Oliveira, R.P., Mendes, B.M.J., Tulmann Neto, A., De- Oliveira, R.P., 1994. Cell suspension culture of two citrus rootstocks. Revista Brasileira de Fisiologia Vegetal, 6: 141-144.
- Pan, Z., Guan, R., Zhu, S., Deng, X.X., 2009. Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck). Plant Cell Rep., 28: 281–289.
- Pang, X.M., Hu, C.G., Deng, X.X., 2007. Phylogenetic relationship within Citrus and related genera as inferred from AFLP markers. Genet. Res. Crop Evol., 54: 429– 436.
- Parthasaraty, V.A., Nagaraju, V., Rahman, S.A.S., 1997. In vitro grafting of Citrus reticulata Blanco. Folia- Horticulturae, 2: 87-90.
- Pavan, A., Calixto, C., Cardoso, S.C., Mendes, B.M.J., Filho, A.B., Lopes, J.R.S., Carvalho, C.R., Filho, F.A.A.M., 2007. Evaluation of ‘Hamlin’ sweet orange + ‘Montenegrina’ mandarin somatic hybrid for tolerance to Xanthomonas axonopodis pv. citri and Xylella fastidiosa. Sci. Hort., 113: 278-285.
- Pena, L., Cervera, M., Juarez, J., Navarro, A., Pina, J.A., Navarro, L., 1997. Genetic transformation of lime (Citrus aurantiifolia Swing.): factors affecting transformation and regeneration. Plant Cell Rep., 16: 731-737.
- Pena, L., Cervera, M., Fagoaga, C., Perez, R., Romero, J., Juarez, J., Pina, J.A., Navarro, L., 2004. Agrobacterium mediated transformation of Citrus. Transgenic crops of the world: essential protocols (eds: Curtis, I.S.). Kluwer Academic Publisher. pp: 454.
- Perez-Molphe-Balch, E., Ochoa-Alejo, N., 1997. In vitro plant regeneration of Mexican lime and mandarin by direct organogenesis. HortScience, 32: 931-934.
- Perez, R.M., Navarro, L., Duran-Vila, N., 1997. Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars. Plant Cell Reports, 17: 44-49.
- Quan, Y., Zhu, S.C., Tian, C.G., Jin, R.R., Shan, C.C., Quan, Y., Chen, Z.S., Guo, T.C., Zhang, J.R., Chen, S.C., 1996. Citrus germplasm preservation in vitro. South China Fruits, 25: 3-5.
- Roose, M.L., 1993. Genetic Mapping in Citrus. Proc. of Int. Mandarin Festival. Azuma-cho. Kagoshima 899-14, Japan. October 29-31.
- Roose, M.L., 2007., Mapping and marker assisted selection in Citrus. In: Khan IA (ed) Citrus genetics, breeding and biotechnology. CAB International, Wallingford, UK, pp 275–286
- Sharma, S., Singh, B., Rani, G., Zaidi, A.A., Hallan, V., Nagpal, A., Virk, G.S., 2007. In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting. In Vitro Cell.Dev.Biol.—Plant., 43: 254–259.
- Singh. S., Rajam, M.V., 2009. Citrus biotechnology: Achievements, limitations and future directions. Physiol. Mol. Biol. Plants, 15: 3-22.
- Seker, M., 1999. Aurantioideae altfamilyasındaki önemli turunçgil genotiplerinin tanılanmasında genom büyüklükleri ve izoenzim analizlerinden yararlanma olanakları. Çukurova Üniv. Fen. Bil. Ens. Adana.
- Shi, J.X., Chen, S., Gollop, N., Goren, R., Goldschmidt, E.E., Porat, R., 2008. Effects of anaerobic stress on the proteome of citrus fruit. Plant Science, 175: 478–486.
- Sim, G.E., Goh, C.J., Loh, C.S., 1989. Micropropagation of Citrus mitis Blanco: multiple bud formation of from shoot and root explants in the presence of 6- benzylaminopurine. Plant Sci., 69: 203-210.
- Spiegel-Roy, P., Ben-Hayyim, G., 1985. Selection and breeding for salinity tolerance in vitro. Plant Soil, 89: 243-252.
- Stam, P., 1993. Constraction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant Journal, 3: 739-744.
- Swingle, W.T., Reece, P.C., 1967. The botany of Citrus and its wild relatives. In: Reuther W, Webber HJ, Batchelor DL (eds). The Citrus Industry, vol. 1. University of California, Berkeley, pp.190-430.
- Tanaka, T., 1977. Fundamental Discussion of Citrus Classification. Stud. Citrol., 14: 1-6
- Tapati, D., Mitra, G.C., Chatterjee, A., Das, T., 1995. Micropropagation of Citrus sinensis var. mosambi - an important scion. Phytomorphology, 45: 57-64.
- Uzun, A., Yesiloğlu, T., Aka-Kacar, Y., Tuzcu, O., Gülsen, O., 2009. Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scien. Hortic., 121: 306-312.
- Xu, X.Y., Liu, J.H., Deng, X.X., 2006a. Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast–protoplast fusion. Genetic Transformation and Hybridization, 25: 533-539.
- Xu, C.J., Bao, L., Zhang, B., Bei, Z.M., Ye, X.Y., Zhang, S.L.,Chen, K.S., 2006b. Parentage analysis of huyou (Citrus changshanensis) based on internal transcribed spacer sequences. Plant Bred., 125: 519-522.
- Vardi, A., Epstein, E., Breiman, A., 1986. Is the Phytopthora citrophthora culture filtrate a reliable tool for the in vitro selection of resistant Citrus variants. Theor. Appl. Genet., 72: 596-574.
- Vardi, A., Gallun, E., 1988. recent advances in protoplast culture in horticultural crops-citrus. Scientia Horticulturae 37: 217-230.
- Vardi, A., Bleichman, S., Aviv, D., 1990. Genetic transformation of Citrus protoplasts and regeneration of transgenic plants. Plant Sci., 69: 199-206.
- Wu, X.B., Wang. J., Liu, J.H., Deng, X.X., 2008. Involvement of polyamine biosynthesis in somatic embryogenesis of Valencia sweet orange (Citrus sinensis) induced by glycerol. J. Plant Phys., 166: 52-62.
- Yamamoto, M., Kobayashi, S., Nakamura,. Y., Yamada, Y., 1993. Phylogenic relationships of Citrus revealed by diversity of cytoplasmic genomes. In: Hayashi T, Omura M, Scott NS (eds). Techniques on gene diagnosis and breeding in fruit trees. Fruit Trees Research Station, Okitsu, Japan, pp.39-46.
- Yang, Z.N., Ingelbrecht, I.L., Louzada, E., Skaria, M., Mirkov, T.E., 2000. Agrobacterium-mediated transformation of the commercially important grapefruit cultivar Rio Red (Citrus paradisi Macf.). Plant Cell Reports, 19:1203–1211.
- Yao, J., Jin, H.W., Gleave, A.P., Morris, B.A.M., Yao, J.L., Wu, L.U., 1996. Transformation of citrus embryogenic cells using particle bombardment and production of transgenic embryos. Plant Science Limerick, 113: 175- 183.
- Zanek, M.C., Reyes, C.A., Cervera, M., Pena, E.J., Velázquez, K., Costa, N., Plata, M.I., Grau, O., Pena, L., García, M.L., 2008. Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. Plant Cell Reports, 27: 57-66.
- Zarei, A., Rahimian, H., 1997. Elimination of citrus tristeza virus from two cultivars of satsuma mandarin through shoot-tip grafting. Iranian J. Plant Pathology., 33: 84-89.