Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Hem sağlık hem de gıda sektörü için önemli bir bitki olan Stevia rebaudiana Bertoni, kalorisi olmayan tatlandırıcı bileşikler içermektedir ve stevia-kaynaklı sentetik olmayan bu tatlandırıcılar için talepte sürekli bir artış söz konusudur. Mevcut çalışmanın amacı, S. rebaudiana'nın in vitro ticari üretimi için yüksek steviosid ve rebaudiosid A içeriğine sahip klonların belirlenmesidir. Bu amaçla, Stevia tohumları in vitro koşullarda kültüre alınmış ve yüksek çoğaltım oranı ve yüksek steviosid ve rebaudiosid A içeriği gösteren klon hatları seçilmiştir. Elde edilen iki klon hattı ticari bir üretici tarafından Stevia'nın ticari üretimi için adapte edilmiştir.

Stevia rebaudiana Bertoni, which is an important plant for both the health and the food sectors, contains non calorie sweetener compounds and there is a continuous increase in the demand for these stevia-based non-synthetic sweeteners. The aim of the present work was to determine clones that have high stevioside and rebaudioside A contents for in vitro commercial production of Stevia rebaudiana. For this purpose, the seeds of Stevia were cultured in vitro and the clone lines that showed the highest multiplication rate and highest stevioside and rebaudioside A contents were selected. Two clone lines obtained were adapted by a commercial producer for the commercial production of Stevia.

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