Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Hem sağlık hem de gıda sektörü için önemli bir bitki olan Stevia rebaudiana Bertoni, kalorisi olmayan tatlandırıcı bileşikler içermektedir ve stevia-kaynaklı sentetik olmayan bu tatlandırıcılar için talepte sürekli bir artış söz konusudur. Mevcut çalışmanın amacı, S. rebaudiana'nın in vitro ticari üretimi için yüksek steviosid ve rebaudiosid A içeriğine sahip klonların belirlenmesidir. Bu amaçla, Stevia tohumları in vitro koşullarda kültüre alınmış ve yüksek çoğaltım oranı ve yüksek steviosid ve rebaudiosid A içeriği gösteren klon hatları seçilmiştir. Elde edilen iki klon hattı ticari bir üretici tarafından Stevia'nın ticari üretimi için adapte edilmiştir.

Stevia rebaudiana Bertoni, which is an important plant for both the health and the food sectors, contains non calorie sweetener compounds and there is a continuous increase in the demand for these stevia-based non-synthetic sweeteners. The aim of the present work was to determine clones that have high stevioside and rebaudioside A contents for in vitro commercial production of Stevia rebaudiana. For this purpose, the seeds of Stevia were cultured in vitro and the clone lines that showed the highest multiplication rate and highest stevioside and rebaudioside A contents were selected. Two clone lines obtained were adapted by a commercial producer for the commercial production of Stevia.

Kaynakça

Ahmed, M. B., M. Salahin, R. Karim, M. A. Razvy, M. M. Hannan, R. Sultana, M. Hossain, and R. Islam. 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni) in Bangladesh. J. Sci. Res. 2: 121-125.

Ali, A., I. Gull, S. Naz, and S. Afghan. 2010. Biochemical investigation during different stages of in vitro propagation of Stevia rebaudiana. Pak. J. Bot. 42 (4): 2827-2837.

Bayraktar, M., S. Hayta, S., Parlak, and A. Gurel. 2015. Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots, Trees, 29 (4): 999-1009. doi: 10.1007/s00468-015- 1179-2.

Bondarev, N., O. Reshetnyak, and A. Nosov. 2001. Peculiarities of diterpenoidsteviol glycoside production in in vitro cultures of Stevia rebaudiana Bertoni. Plant Sci. 161: 155-163.

Bondarev, N., O. Reshetnyak, and A. Nosov. 2003. Effects of nutrient medium composition on development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides. Plant Sci. 165: 845-850

Brandle, J. E., A. N. Starrat, and M. Gijen. 1998. Stevia rebaudiana: Its agricultural, biological, and chemical properties. Can. J. of Plant Sci. 78: 527-536.

Brandle, J. E., and P. G. Telme. 2007. Steviol glycoside biosynthesis.Phtochemistry 68: 1855-1863.

Erkucuk, A., I. H. Akgun, and O. Y. Celiktas. 2009. Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J. Supercrit. Fluid. 51: 29-35.

Gamborg, O. L., R. A. Miller, and K. Ojima.1968. Nutrient requirements of suspension cultures of soybean root cells. Exp.Cell. Res. 50 (1): 151-158.

Giridhar, P., K. S. Sowmya, A. Ramakrishna, and G. A. Ravishankar. 2010. Rapid clonal propagation and stevioside profiles of Stevia rebaudiana Bertoni. Int. J. Plant Dev. Biol. 4 (1): 47-52

Hwang, S. J. 2006. Rapid in vitro propagation and enhanced stevioside accumulation in Stevia rebaudiana Bert. J. Plant Biol. 49 (4): 267-270.

Ibrahim, I. A., M. I. Nasr, B. R. Mohammed, and M. M. ElZefzafi. 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Tech. 10: 254-259.

Khalil, S. A., R. Zamir, and N. Ahmad. 2014. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni). Saud. J. Biol. Sci. 21 (6): 566-573.

Koyama, E., K. Kitazawa, Y. Ohori, O. Izawa, K. Kakegawa, A. Fujino, and M. Ui. 2003. In vitro metabolism of the glycosidics weeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora. Food and Chemical Toxicology. 41: 359- 374.

Ladygin, V. G., N. I. Bondarev, G. A. Semenova, A. A. Smolov, O. V. Reshetnyak, and A. M. Nosov. 2008. Chloroplast ultrastructure, photosynthetic apparatus activities and production of steviol glycosides in Stevia rebaudiana in vivo and in vitro. Biologia Plantarum 52: 9-16.

Lata, H., S. Chandra, Y. H. Wang, V. Raman, and I. A. Khan. 2013. TDZ-induced high frequency plant regeneration through direct shoot organogenesis in Stevia rebaudianaBertoni: an important medicinal plant and a natural sweetener. Am. J. Plant Sci. 4: 117-128.

Lemus-Mondaca, R., A. Vega-Gálvez, L. Zura-Bravo, and K. Ah-Hen. 2012. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem. 132: 1121-1132.

Lloyd, G., and B. McCown. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. 30: 421-427.

Madan, S., S. Ahmad, G. N. Singh, K. Kohli, Y. Kumar, R. Singh, and M. Garg. 2010. Stevia rebaudiana (Bert.) Bertoni - A Review.Indian J. Nat. Prod. Res. 1 (3): 267-286.

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