Antibacterial Activity of Chitosan, Some Plant Seed Extracts and Oils Against Escherichia coli and Staphylococcus aureus

In this present study, various concentrations (0.5, 1, and 2%) of chitosan extracted through the chemical methods from the shells of crayfish (Astacus leptodactylus), pink shrimp (Parapenaeus longirostris), blue crab (Callinectes sapidus) shells, methanolic extracts of black cumin (Nigella sativa L), flaxseed (Linum usitatissimum), Chaste Tree (Vitex agnus-castus L.) and black cumin and flaxseed oil were tested in vitro for their antibacterial activities against two pathogenic bacteria Escherichia coli and Staphylococcus aureus, using the disk diffusion method. On the other hand, were Erythromycin and florfenicol were used as a positive control. The negative control was Acetic Acid and cotton oil. Antimicrobial activity of chitosan is affected by different intrinsic and extrinsic factors. Chitosan source, molecular weight, deacetylation degree, viscosity, and solvent material, pH, ionic strength, metal ions, and bacteria cultures. In the present study, all four extracted chitosans were showed different antimicrobial effects on two different types of bacteria, while there are not any antibacterial effect of aqueous extracts and oils of the three plants seeds that used in this study. This is the first report concerning the antimicrobial activity of chitosan compared to some plant seed extracts and oils against Escherichia coli and Staphylococcus aureus. Furthermore, results showed the chitosan of this species might be an alternative as an antimicrobial agent for the pharmaceutical industry.


Al-Manhel, A. J., Al-Hilphy, A. R. S. & Niamah, A. K. (2018). Extraction of chitosan, characterisation and its use for water purification. Journal of the Saudi Society of Agricultural Sciences, 17(2): 186-190.

Al-Reza, S. M., Rahman, A., Lee, J. & Kang, S. C. (2010). Potential roles of essential oil and organic extracts of Zizyphus jujuba in inhibiting food-borne pathogens. Food Chemistry, 119(3): 981-986.

Bauer, A. W. (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, 45: 149-158.

Benhabiles, M. S., Salah, R., Lounici, H., Drouiche, N., Goosen, M. F. A. & Mameri, N. (2012). Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food hydrocolloids, 29(1): 48-56.

Bilen, S., Ünal, S. & Güvensoy, H. (2016) Effects of oyster mushroom (Pleurotus ostreatus) (Urtica dioica) methanolic extracts on immune responses and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). Aquaculture, 454: 90-94.

Boonlertnirun, S., Boonraung, C. & Suvanasara, R. (2017). Application of chitosan in rice production. Journal of metals, materials and minerals, 18(2).

Calsamiglia, S., Busquet, M., Cardozo, P. W., Castillejos, L. & Ferret, A. (2007). Invited review: essential oils as modifiers of rumen microbial fermentation. Journal of dairy science, 90(6): 2580-2595.

Cheng, S., Huang, C., Chen, Y., Yu, J., Chen, W. & Chang, S. (2009). Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. Bio resource Technology, 100: 452–456.

Darmadji, P. & Izumimoto, M. (1994). Effect of chitosan in meat preservation. Meat science, 38(2): 243-254.

Hemaiswarya, S., Kruthiventi, A. K. & Doble, M. (2008). Synergism between natural products and antibiotics against infectious diseases. Phytomedicine, 15(8): 639- 652.

Hongpattarakere, T. & Riyaphan, O. (2008). Effect of deacetylation conditions on antimicrobial activity of chitosans prepared from carapace of black tiger shrimp. Songklanakarin Journal of Science & Technology, 30.

Jeon, Y. J., Park, P. J. & Kim, S. K. (2001). Antimicrobial effect of chitooligosaccharides produced by bioreactor. Carbohydrate polymers, 44(1): 71-76.

Küçükgülmez, A., Gülnaz, O., Celik, M., Yanar, Y., Kadak, A. E. & Gerçek, G. (2012). Antimicrobial activity of the chitosan extracted from Metapenaeus stebbingi shell wastes. Journal of Polymers and the Environment, 20(2): 431-437.

Küçükgülmez, A., Kadak, A. E. & Gökçin, M. (2013). Antioxidative and antimicrobial activities of shrimp chitosan on gilthead sea bream (Sparus aurata) during refrigerated storage. International journal of food science & technology, 48(1): 51-57.

Kurita, K., Akao, H., Yang, J. & Shimojoh, M. (2003). Nonnatural branched polysaccharides: synthesis and properties of chitin and chitosan having disaccharide maltose branches. Biomacromolecules, 4(5): 1264- 1268.

Mukherjee, D. P. (2001). U.S. Patent No. 6,310,188. Washington, DC: U.S. Patent and Trademark Office.

Muzzarelli, R. A. A. (1985). Chitin. In: Aspinall, G.O. (Ed.), The Polysaccharides. Academic Press, New York, pp. 417– 450.

No, H. K. & Meyers, S. P. (1995). Preparation and characterization of chitin and chitosan – a review. Journal of Aquatic Food Product Technology, 4(2): 27– 52.

Noor, A., Bansal, V. S. & Vijayalakshmi, M. A. (2013). Current update on anti-diabetic biomolecules from key traditional Indian medicinal plants. Current science, 721-727.

Pakravan, S., Hajimoradloo, A. & Ghorbani, R. (2012) Effect of dietary willow herb, Epilobium hirsutum extract on growth performance, body composition, haematological parameters and Aeromonas hydrophila challenge on common carp, Cyprinus carpio. Aquaculture Research, 43: 861-869.

Phillipson, J. D. (1994) Natural products as drugs. Transactions of the Royal Society of Tropical Medicine and Hygiene, 88: 17–19.

Rabea, E. I., Badawy, M. E. T., Stevens, C. V., Smagghe, G. & Steurbaut, W. (2003). Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules, 4(6): 1457-1465.

Ray, A. B., Sarma, B. K. & Singh, U. P. (2004) Medicinal properties of Plants: Antifungal, Antibacterial and Antiviral Activities. Lucknow, International Book 600 pp.

Seo, S., King, J. M., Prinyawiwatkul, W. & Janes, M. (2008). Antibacterial activity of ozone‐depolymerized crawfish chitosan. Journal of food science, 73(8): M400-M404.

Seo, S. W. (2006). Depolymerization and Decolorization of Chitosan by Ozone Treatment. Chung-Ang University, Master Thesis.

Singh, G., Marimuthu, P., de Heluani, C. S. & Catalan, C. (2005a). Chemical constituents and antimicrobial and antioxidant potentials of essential oil and acetone extract of Nigella sativa seeds. Journal of the Science of Food and Agriculture, 85(13): 2297-2306.

Singh, G., Maurya, S., Catalan, C. & De Lampasona, M. P. (2005b). Studies on essential oils, Part 42: chemical, antifungal, antioxidant and sprout suppressant studies on ginger essential oil and its oleoresin. Flavour and fragrance journal, 20(1): 1-6.

Škrinjar, M. M. & Nemet, N. T. (2009). Antimicrobial effects of spices and herbs essential oils. Acta periodica technologica, (40): 195-209.

Tajik, H., Moradi, M., Rohani, S. M. R., Erfani, A. M. & Jalali, F. S. S. (2008). Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the product. Molecules, 13(6): 1263-1274.

Tsai, G. J., Zhang, S. L. & Shieh, P. L. (2004). Antimicrobial activity of a low-molecular-weight chitosan obtained from cellulase digestion of chitosan. Journal of food protection, 67(2): 396-398.

Viegas, C. & Bolzani, V. S. (2006). Os produtos naturais e a química medicinal moderna. Química Nova, 29: 326- 337.

WHO. (1996). The World health report: 1996: fighting disease, fostering development. World Health Organization. Yang, T. C., Chou, C. C. & Li, C. F. (2005). Antibacterial activity of N-alkylated disaccharide chitosan derivatives. International Journal of Food Microbiology, 97(3): 237-245.

Zheng, L. Y. & Zhu, J. F. (2003). Study on antimicrobial activity of chitosan with different molecular weights. Carbohydrate polymers, 54(4): 527-530.

Kaynak Göster

Alınteri Zirai Bilimler Dergisi
  • ISSN: 2564-7814
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 2007