MEHMET KARACA

Fiber development characteristics of cotton mutants: fuzzless-lintless, ligon lintless and fuzzless-lint

Bu çalışmanın ana amacı üç farklı mutant pamuğun; linter (hay) ve lint üretmeyen resesif mutant lintersiz-lintsiz (fl), kısa lifler veren ve tek bir gen tarafından kontrol edilen, seçkin bir dominant Ligon lintless $(Li_1)$ ve yalnızca lint üreten lintersiz fuzzless-lint (f-142) lif oluşumunu başlatma'öîielliklerihin karşı laştırılmasıdır. Çalışmada bir Tarama Elektron Miksroskop (TEM) kullanılarak lif oluşumundaki değişiklikler izlenmiştir. Sonuçlar $Li_1$ ve f-142'nin çiçeklenmeden 0, 1 ve 3 gün sonraki lif gelişiminin normal,Teksas Markır (TM-1) pamuğuna çok benzediğini göstermiştir. Ancak ileriki dönemlerde lif uzaması mutant ovüllerinde çiçeklenmeden 8-10 gün sonra dururken normal $TM_1$ ve mutant fiızzless-lint'de devam etmiştir. Lintersiz-Lintsiz mutant ovüllerinde ise linter yada lint gelişememiştir. Lintersiz-Lintsiz, lintersiz-lint ve $Li_1$ mutantlarının kullanılması açıkça göstermiştir ki linter ve lint lifleri aynı zamanda gelişmeye başlamakta ancak(bunlar farklı genler tarafından kontrol edilmektedir. Bu çalışma yine mutant $Li_1$ geninin ileriki dönemlerinde, tahminen ileri lif uzama döneminde aktif olduğunu göstermiştir.

Anahtar Kelimeler:

pamuk, mutant, taramalı elektron mikroskopi, bitki püskülü, lif, lif özellikleri

Pamuk lif mutantlarının gelişim özellikleri: lintersiz-lintsiz, ligon lintsiz ve lintersiz-lint

The main objective of this research was to compare fiber initiation properties of 3 different cotton mutants; fuzzless-lintless (fl), recessive mutant which does not produce fuzz and lint .fibers, Ligon lintless $(Li_1)$, a monogenic novel dominant mutant producing only short fibers (short fuzz and lint fibers) an&fuzzless-lint (f-142) which only produces lint fibers. In this research, a Scanning Electrpn Microscopy (SEM) was used to monitor the changes in the fiber initiations. Studies indicated that the fiber initiation of $Li_1$ and f-142 at 0,1 and 3 days post anthesis (DPA) were very similar with that of the normal cotton, Texas Marker 1, (TM|). However, at later stages fiber elongation stopped in the $Li_1$ ovules at about 8-10 DPA, whereas it continued in the $TM_1$ and f-l42 ovules. The fuzzless-lintless ovules failed to produce both fuzz and lint fibers. The use offitzzless-lint, fiizzless-lintless and $Li_1$ mutants clearly indicated that fuzz fibers and lint fibers initiate at the same time but they are controlled by different genes. Present study also suggested that $Li_1$ gene Is active during the latter stages of fiber development, probably during the later elongation phase.

Keywords:

cotton, mutants, scanning electron microscopy, lint, fibre, fibre properties,

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