Kolorektal kanserde lncRNA DPP10-AS1 ekspresyonu tümör baskılayıcı ve tümör mikroçevresinde metabolit transportuyla ilişkili olabilir mi?

Giriş ve amaç: Onkogen ve tümör baskılayıcı gen ifadeleri başta olmak üzere birçok RNA ve proteinle moleküler etkileşimde bulunabilen lncRNA’lar solid organ kanserlerinde ilişkilendirilmeye başlanmıştır. Deneysel çalışmalarda Wnt/β -katenin yolağı ile ilişkilendirilen lncRNA DPP10-AS1’in çalışmamızda kolorektal tümörlerdeki ifadesi ve serum biyobelirteçleriyle olan ilişkisinin incelenmesi amaçlanmıştır. Gereç ve yöntem: Kolorektal kanser tanılı elli bir olgunun tümör ve tümörsüz çevre dokusuna ait formalinle fikse edilmiş parafine emdirilmiş bloklarından total RNA izolasyonu gerçekleştirildi. İzole edilen total RNA’dan lncRNA’lara spesifik c-DNA sentezi gerçekleştirildikten sonra DPP10-AS1’e özgü primerler ile gerçek zamanlı polimeraz zincir reaksiyonu ile ekspresyon düzeyi tespit edildi. Ekspresyon düzeyleri olguların serum biyomarkerlarıyla korelasyon yönünden incelendi. Bulgular: lncRNA DPP10-AS1 ‘in katlı değişimi tümörsüz çevre dokusunda tümör dokusuna göre yaklaşık 5,7 kat yüksek olduğu tespit edilmiştir (P=0,0002). Histopatolojik bulgularla lncRNA DPP10-AS1 arasında istatistiksel bir farklılık tespit edilmezken, uzak organ metastazı olmayanlarda olanlara göre 1,5 kat yüksek olduğu izlenmiştir (p>0.05). DPP10-AS1 ile albümin (r: ,403; p=0,033) ve amilaz (r: ,450; p= 0,031) arasında pozitif korelasyonları tespit edildi. Sonuç: DPP10-AS1’in tümör baskılayıcı rolü olduğunu, albüminle korelasyonu tümör mikroçevresinde sekonder metabolitlerin transportunda rol oynayabileceğini düşündürmektedir. DPP10-AS1 ile amilaz korelasyonunun literatürde tanımlanan yüksek amilaz düzeylerinin tümör farklılaşması, çoğalması sürecindeki etkisiyle paralele şekilde tümör mikroçevresinin bir yanıtı olarak ifade edilebileceğini düşünmekteyiz.

Anahtar Kelimeler:

lncRNA, DPP10-AS1, Amilaz, Albümin, Kolorektal kanser

Could lncRNA DPP10-AS1 expression in colorectal cancer be associated with tumor suppressor and metabolite transport in the tumor microenvironment?

Background and aims: lncRNAs, which can interact with many RNAs and proteins, especially oncogene and tumor suppressor gene expressions, have begun to be associated in solid organ cancers. We aimed to examine the expression of lncRNA DPP10-AS1, which is associated with the Wnt/β-catenin pathway in experimental studies, in colorectal tumors and its relationship with serum biomarkers. Materials and methods: Total RNA isolation was performed from formalin-fixed paraffin-impregnated blocks of tumor and tumor-free surrounding tissue of fifty-one patients with colorectal cancer. After specific c-DNA synthesis was performed from the isolated total RNA to lncRNAs, the expression level was determined by real-time polymerase chain reaction with primers specific to DPP10-AS1. Expression levels were analyzed in terms of correlation with serum biomarkers of the cases. Results: The fold change of lncRNA DPP10-AS1 was found to be approximately 5.7 times higher in tumor-free surrounding tissue than in tumor tissue (P=0.0002). While no statistical difference was detected between histopathological findings and lncRNA DPP10-AS1, it was observed that it was 1.5 times higher in patients without distant organ metastasis (p>0.05). Positive correlations were detected between DPP10-AS1 and albumin (r: .403; p=0.033) and amylase (r: .450; p= 0.031). Conclusion: DPP10-AS1 has a tumor suppressive role, and its correlation with albumin suggests that it may play a role in the transport of secondary metabolites in the tumor microenvironment. We think that the correlation between DPP10-AS1 and amylase can be expressed as a response of the tumor microenvironment in parallel with the effect of high amylase levels on tumor differentiation and proliferation, as defined in the literature.

Keywords:

lncRNA, DPP10-AS1, Amylase, Albumin, Colorectal cancer,

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