Kolesterol Tayini için Biyosensör Geliştirilmesi

Bu çalışmada, kolesterol amperometrik tayini için Prusya mavisi (PB) temelli amperometrik biyosensörler hazırlandı. Bu amaçla, jelatin ve gluteraldehit içeren ortam kullanılarak çapraz bağlama tekniği ile PB ve kolesterol oksidaz (COx) enzimi screen-printed elektrotların (SPE) yüzeyine immobilize edildi. SPE/PB/COx elektrotların kolesterol tayini için uygun oldukları gözlendi ve optimizasyon çalışmaları bu enzim elektrotları kullanılarak yapıldı. SPE/PB/COx enzim elektrodu için optimum jelatin miktarı, gluteraldehit oranı, PB derişimi, tampon cinsi, tampon derişimi, sırasıyla fosfat tamponu; 0,002 g, % 0.5, 0.02 M, 0.05 M; pH:7,5 olarak belirlendi. Enzim elektrodun, kolesterol için doğrusal çalışma aralığı 2×10-6-2×10-5 M, gözlenebilme sınırı 2×10-6 M, cevap süresi 50 s ve raf ömrü yaklaşık bir ay olarak bulundu. SPE/PB/COX kolesterol biyosensörü hazırlanması optimizasyonu başarılı bir şekilde elde edildi.

Development of Biosensors for Cholesterol Determination

In this study, for the determination of cholesterol amperometric Prussian blue (PB) based amperometric biosensors were prepared. For this purpose, the cross-linking techniques using glutaraldehyde medium containing gelatin and PB and cholesterol oxidase (COX) enzyme was immobilized on the surface of the screen-printed electrode. SPE / PB / COX was observed that the electrodes are suitable for the determination of cholesterol and optimization study was performed using the enzyme electrodes. SPE / PB / COX enzyme optimum amount of gelatin to the electrodes, glutaraldehyde ratio of concentration, buffer type, buffer concentration, respectively, in phosphate buffer; 0.002 g, 0.5%, 0.02 M, 0.05 M; pH was set at 7.5. The enzyme electrode linear operating range for cholesterol, 2 × 10-6-2 × 10-5 M, observed ability to limit 2 × 10-6 M, response time of 50 h and shelf-life was found to be about a month. Optimization of SPE/PB/COX cholesterol biosensor preparation was successfully achieved.

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  • Bailey J.E., Ollis F.D. 1986. Biochemical Engineering Fundementals 2nd ed. McGraw Hill International Editions, 984.
  • Basu A. K., Chattopadhyay P., Roychoudhuri U., Chakraborty R. 2007. Development Of Cholesterol Biosensor Based On Immobilized Cholesterol Esterase And Cholesterol Oxidase On Oxygen Electrode For The Determination Of Total Cholesterol In Food Samples. Bioelectrochemistry, 70: 375–379.
  • Bokoch M.P., Devadoss A., Palencsar M.S., Burgess J.D. 2004. Steady-State Oxidation Of Cholesterol Catalyzed By Cholesterol Oxidase İn Lipid Bilayer Membranes On Platinum Electrodes. Analytica Chimica Acta, 519: 47-55.
  • Brahim S., Narinesing D., Guiseppi-Elie A. 2001. Amperometric Determination Of Cholesterol In Serum Using Biosensor Of Cholesterol Oxidase Contained Within A Polypyrrole-hydrogel Membrane. Analytica Chimica Acta, 448(1-2): 27-36.
  • Chauhan N., Pundir C. S. 2011. Co-Immobilization Of Cholesterol Esterase, Cholesterol Oxidase And Peroxidase On Pvc Strip For Serum Cholesterol Determination. Anal Methods, 3 :1360–1365.
  • Fan, Q., Shan, D., Xue, H., He, Y. and Cosnier, S. 2007. Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix. Biosensors and Bioelectronics, 22, 816-821.
  • Fujishiro K., Uchida H., Shimokawa K., Nakano M., Sano F., Ohta T., Kayahara N., Aisaka K., Uwajima T. 2002. Purification And Propperties On A New Brevibacterium Sterolicum Cholesterol Oxidase Produce By E.Coli Mm294/Pnh10. FEMS Microbiology Letters, 215: 243-248.
  • Huy N.L., Thuy N.T., Binh N.H., Thinh N.N., Lam T.D. 2013. Covalent Immobilization Of Cholesterol Oxidase and Poly(styrene-co-acrylic acid) Magnetic Microspheres On Polyaniline Films For Amperometric Cholesterol Biosensing. Anal Methods, 5(6): 1392-1398.
  • Isobe K., Shoji K., Nakanıshı Y., Yokoe M., Wakao N. 2003. Purification and Some Properties Of Cholesterol Oxidase Stable İn Detergents Frod Γ-Probacterium Y-134. Journal of Biosicience and Bioengineering, 95(3): 257-263.
  • Kochana, J., Nowak, P., Jarosz-Wilkolazka, A.J., Bioeron, M. 2008. Tyrosinase/laccase bienzyme biosensor for amperometric determination of phenolic compounds. Microchemical Journal, 89, 171-174.
  • Kumar S., Singh J., Agrawal V. V., Malhotra B. D. 2011. Biocompatible Self-Assembled Monolayer Platform Based On (3-Glycidoxypropyl)Trimethoxysilane For Total Cholesterol Estimation. Anal Methods, 3:2237–2245.
  • Lin C.L, Liao L.C. 2014. Preparation And Characterization Of Micropatterned Prussian Blue Thin Films With Enhanced Electrochromic Properties. Surface and Coatings Technology, 259, B, 330-334.
  • Nishiya Y., Hirayama N. 1999. Alteration Of Substrate Affinity Of Streptomyces Cholesterol Oxidase Application To The Rate Assay Of Cholesterol Serum. Clinica Chimica Acta, 287: 111-122.
  • Özer B.C., Özyörük H., Çelebi S.S., Yıldız A. 2007. Amperometric Enzyme Electrode For Free Cholesterol Determination Prepared With Cholesterol Oxidase Immobilized In Poly (Vinylferrocenium) Film. Enzyme and Microbial Technology, 40(2): 262-265.
  • Rajesh, Takashima, W. and Kaneto, K. 2004. Amperometric phenol biosensor based on covalent immobilization of tyrosinase onto an electrochemically prepared novel copolymer poly(N-3-aminoproply pyrrole-co-pyrrole) film. Sensors and Actuators B, 102, 271-277.
  • Shan, D., Zhang, J., Xue, H.G., Zhang, Y.C., Cosnier, S. and Ding, S.N. 2009. Polycyrstalline bismuth oxide films for development of amperometric biosensensor. Biosensors and Bioelectronics, 24, 2671-3676.
  • Singh K., Basu T., Solanki P.R., Malhotra B.D. 2009. Poly (Pyrrole-Co-N-Methyl Pyrrole) For Application To Cholesterol Sensor. Journal Of Materials Science, 44: 954–961.
  • Singh S., Solanki P.R., Pandey M.K., Malhotra B.D. 2006a. Covelent Immobilization Of Cholesterol Esterase And Cholesterol Oxidase On Polyaniline Films For Application To Cholesterol Biosensor. Analytica Chimica Acta, 568(1-2 ): 126-132.
  • Singh S., Chaubey A., Malhotra B.D. 2004. Amperometric Cholesterol Biosensor Based On Immobilized Cholesterol Esterase And Cholesterol Oxidase On Conduction Polypyrrole Films. Analytica Chimica Acta, 502: 229-234.
  • Solanki P.R., Arya S.K., Singh S.P., Pandey M.K., Malhotra B.D. 2007a. Application Of Electrochemically Prepared Poly-N-Methylpyrrole-P-Toluene Sulphonate Films To Cholesterol Biosensor. Sensors and Actuators B: Chemical, 123(2): 829-839.
  • Yıldırımoglu F. 2009. Kolesterol Tayini İçin Yeni Bir Biyosensör Hazırlanması. Yüksek Lisans Tezi. Gazi Üniversitesi, Türkiye.
  • Telefoncu A. 1999. Biyoreseptör İmmobilizasyonu, Biyosensörler. Biyokimya Lisans Üstü Yazokulu. 42-61.
  • Tembe, S., Karve, M., Inamdar, S., Haram, S., Melo, J. and D’Souza, S. 2006. Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film. Analytical Biochemistry, 349, 72-77.
  • Türkarslan Ö., Kayahan S.K., Toppare L. 2009. Poly(Pyrrole) Versus Poly(3,4-Ethylenedioxythiophene): Amperometric Cholesterol Biosensor Matrices. J Solid State Electrochem, 13: 657–663.
  • Vérdine, C., Fabiano, S. and Tran-Minh, C. 2003. Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support. Talanta, 59, 535-544.
  • Wang H., Mu S. 1999. Bioelectrochemical Characteristics Of Cholesterol Immobilized In A Polyaniline Film. Sensors and Actuators B: Chemical, 56(1-2): 22-30.