Tuğba SARDOHAN KÖSEOĞLU, Atilla EVCİN, Esengül KIR, Burcu ÖZCAN, Nalan Çiçek BEZİR, İsmail ÖZMEN

Gümüş Nitrat Katkılı Modifiye Nanofiber Membranların Hazırlanması, Karakterizasyonu ve Antibakteriyel Özellikleri

Son yıllarda, fonksiyonelleştirilmiş, elektro lif çekimi yöntemi ile hazırlanmış nanofiber membranlar ile antibakteriyel malzeme üretimi oldukça dikkat çekmektedir. Modifiye edilmiş nanofiber membranların üretiminde polimerik materyaller ile nanopartiküllerin kombinasyonu gerçekleştirilmektedir. Ag, Cu, Ce, ZnO ve TiO2 gibi birçok nanopartikülün kullanımı artmış ve antibakteriyel aktiviteleri üzerine çalışmaları hız kazanmıştır. Bu nedenle, bu çalışmada, elektro lif çekimi tekniği kullanılarak poliviniliden florür (PVDF) destek tabakası üzerine gümüş nitrat ilavesi ile modifiye nanofiber membranlar hazırlanmıştır. Kitosan (CS), polivinil alkol (PVA), polivinil prolidon (PVP) ve TiO2 gibi kimyasallar bu membranların hazırlanmasında kullanılmıştır. Modifiye nanofiber membranların karakterizasyonu ise taramalı elektron mikroskobu (SEM), enerji dağılım X-ışınları spektroskopisi (EDS) ve atomik güç mikroskobu (AFM) teknikleri kullanılarak gerçekleştirilmiştir. Karakterizasyon teknikleri, hazırlanan nanofiber membranların nano yapıda olduklarını kanıtlamıştır.Farklı miktarlarda AgNO3 içeren modifiye nanofiber membranların antibakteriyel özellikleri, seçilen E. coli (25922), S. aureus (ATCC 25923, ATCC 6538) ve P. aeruginosa (ATCC 35032) bakterilerine karşı iki farklı metot kullanılarak (Agar disk difüzyon ve yayma plak) test edilmiştir. Sonuç olarak, modifiye nanofiber membranların antibakteriyel aktiviteleri, gümüş iyonlarının ilavesi ile tüm bakterilere karşı artmıştır.

Preparation, Characterization and Antibacterial Properties of Silver Nitrate Doped Modified Nanofiber Membranes

In recent years, production of antibacterial material using functionalized nanofiber membranes prepared by electro-spinning method has attracted much attention. In the production of modified nanofiber membranes, the combination of polymeric materials with nanoparticles has been performed. The use of many nanoparticles such as Ag, Cu, Ce, ZnO and TiO2 has increased and studies on their antibacterial activities have accelerated. Therefore, in this study, modified nanofiber membranes were prepared by adding silver nitrate on the polyvinylidene fluoride (PVDF) support layer using electrospinning technique. Chitosan (CS), poly(vinyl alcohol) (PVA), polyvinyl prolidone (PVP) and TiO2 were used to prepare these membranes. Characterization of the modified nanofiber membranes was also performed by using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and atomic force microscopy (AFM) techniques. The characterization techniques have proven that the prepared nanofiber membranes are nano-structured. The antibacterial properties of modified nanofiber membranes containing different amounts of AgNO3 were tested against selected E. coli (25922), S. aureus (ATCC 25923, ATCC 6538) and P. aeruginosa (ATCC 35032) bacteria by using two different methods (Agar disc diffusion and Baird Parker agar plate). As a result, the antibacterial activities of modified nanofiber membranes increased against all bacteria with the addition of silver ions.

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