Ayşe BULUT, Faik Serhat ÖZSOY, Gülçin AKCA, Nazime TUNCAY, Özgür Yıldırım TORUN, Ömer Engin BULUT

Hidrojen Peroksit/Kolloidal Gümüş’ün Staphylococcus aureus Üzerindeki Etkisi: Pilot Çalışma

Amaç: Mevcut çalışmanın amacı, rutin diş tedavisi sırasında diş hekimlerinin yakın çevresindeki S. aureus aerosollerinin dağılımını belirlemeyi ve hidrojen peroksit/kolloidal gümüş dezenfektanı kullanarak S. aureus kolonizasyonundaki azalmayı araştırmaktır. Gereç ve Yöntem: Çalışma iki gruba ayrılmıştır. Grup A'da (n=90 petri kabı) steril distile su, Grup B'de (n = 90 petri kabı) hidrojen peroksit/kolloidal gümüş ilaveli steril distile su kullanılmıştır. Petri kapları, saat 6 ve 9 pozisyonlarına karşılık gelen bir kafa fantomu üzerine iki yönde ayrı ayrı yerleştirilmiştir. 50 cm, 100 cm ve 200 cm mesafelere kadar üç petri kabı yan yana yerleştirilip S. aureus (ATCC 29213) solüsyonu ile kontamine edilmiştir. Veri analizlerinde Mann Whitney U testi kullanılmıştır. Bulgular: Grup B'de S. aureus bakteri sayısı Grup A’ya göre 50 cm, 100 cm ve 200 cm mesafelere kadar saat 6 ve 9 pozisyonlarına karşılık gelen yönlerde istatistiksel olarak anlamlı derecede azalmıştır (p = 0.00). Sonuç: Hidrojen Peroksit/Kolloidal-Ag dental işlemlerde S. aureus'a karşı destekleyici önlem olarak güvenle kullanılabilir.

Anahtar Kelimeler:

Hidrojen peroksit/kolloidal gümüş, Kontaminasyon, Staphylococcus aureus

The Effect of Hydrogen Peroxide/Colloidal-Ag on Staphylococcus aureus: A Pilot Study

Objective: To determine the distribution of Staphylococcus aureus (S. aureus) aerosols in the immediate environment of dental operators during routine dental treatment and to investigate the reduction in S. aureus colonization using 0.5% hydrogen peroxide/colloidal silver disinfectant were aimed. Materials and Methods: The study was divided into two groups. Sterile distilled water was used in Group A (n = 90 Petri plates). Sterile distilled water with hydrogen peroxide/colloidal silver was used in Group B (n = 90 Petri plates). The plates were set up seperately in two directions on the head phantom corresponding to the 6 and 9 o’clock positions. Three petri plates were placed side by side up to 50 cm, 100 cm and 200 cm distances, and the experimental design were proceeded by contaminating with S. aureus (ATCC 29213). Mann Whitney U test was used for comparing to independent samples. Results: When compared to Group A, S. aureus grown colonies on agar plates in Group B was significantly decreased in directions corresponding to the 6 and 9 o’clock positions to 50 cm, 100 cm, and 200 cm distances (p = 0.00). Conclusion: H2O2/Colloidal-Ag can safely be used as a supportive precaution against S. aureus.

Keywords:

Contamination, Hydrogen peroxide/colloidal silver, Staphylococcus aureus,

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