Semen tespitinde farklı yöntemlerin karşılaştırılması

Adli bilimler, cinsel saldırı olgularında çok büyük bir rol üstlenmektedir. Cinsel saldırı iddiasını kesin ispatlayacak, sanığa ait semen ve/veya spermatozoa, mağdurun vücut boşluklarından, giysilerinden ya da olay yerinden elde edilebilir. Vücut boşluklarındaki semen veya spermatozoa, drenajla veya kişinin yıkamasıyla dilüe olarak ya eser miktarda kalacaktır ya da yok olacaktır. Bu çalışmada bu ölçüt göz önünde bulundurularak, rastgele seçilmiş 20 gönüllü katılımcıdan (yaş aralığı 20-57) cinsel perhizli ve perhizsiz örnekler alınmış ve farklı oranlarda dilüe edilmişlerdir. Çalışmanın ilk aşamasında spermatozoonlar, morfolojik olarak Corin-Stockis, Field Rapid Blue-Red ve Eosin-Nigrosin boyaları ile gösterilmiştir. İkinci aşamada ise adlî bilimler açısından semenin ispatında önemli bir belirteç olan PSA, Seratec PSA Semiquant test kullanılarak gösterilmiştir. Çalışmanın sonucunda, cinsel perhizli örneklerde daha fazla spermatozoa olduğu ve boyaların var olan spermatozoayı gösterdiği, ancak en iyi boyamanın Field Rapid Blue-Red boyası ile gerçekleştiği gözlemlenmiştir. Çok yüksek dilüsyonlarda spermatozoayı morfolojik olarak göstermek mümkün olmaz iken PSA konsantrasyon ölçümü semenin varlığını ispatlamada başarılı olmuştur.

Comparison of different semen determination methods

Forensic science is a major role in solving sexual assault cases. The semen or spermatozoa that originated from the assailant, which will completely prove the sexual assault claim. The semen and/or spermatozoa can be collected from victim's body cavities, clothes and crime scene. The semen or spermatozoa in the body cavities can be diluted or destroyed by draining or showering by the victim. Keeping this criterion in mind we configured the study accordingly. We first collected fresh semen samples from 20 randomly selected volunteer participants (age range 20-57). We obtained the semen samples in two states. The first semen sample was collected a minimum of 72 hours after ejaculation, the second sample was collected maximum 24 hours after ejaculation. We diluted the fresh semen samples with different ratios; we evaluated the samples with two different methods. In the first method of study we made the spermatozoids visible by staining them with Corin-Stockis, Field Rapid Blue-Red and Eosin-Nigrosin stains. In the second method we evaluated PSA concentration, which is a remarkable marker for forensic science in demonstrating semen, with Seratec PSA Semiquant test. In conclusion, we found out that, spermatozoa concentration in sexual absistance is high and all morphology stains make spermatozoa visible, but the most effective stain was Field Rapid Blue-Red stain. In high dilutions, spermatozoa can not be seen nor observed as morphologically however, evaluation of PSA concentration has great success to prove semen presence.

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ISSN: 1018-5275
Başlangıç: 1985
Yayıncı: BAYT Yayıncılık

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