ABSTRACTThe present study was undertaken to investigate the incidence of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 in 350 composite milk samples (four quarters in one collection vial) obtained from five different farms in Istanbul. Each sample was aseptically taken straight from udder of individual cattle. The results showed that 0.57% (2/350) of milk samples were contaminated with L. monocytogenes. Salmonella spp. and E. coli O157:H7 were not detected in any samples. In conclusion, considering that the mortality of listeriosis is very high, low prevalence rate of L. monocytogenes should not be disregarded. Therefore, it is essential to ensure improving the quality of production technology and developing the sanitation strategies for enhancing the safety of food. Keywords: Raw Milk, Listeria monocytogenes, Salmonella spp., Escherichia coli O157:H7