A well-differentiated invasive ocular squamous cell carcinoma (OSCC) localized in the conjunctiva and medial peribulbar re-gion, including the third eyelid, was diagnosed in the left eye of a 5-year-old Holstein cow causing ocular pain. Marginal re-section of the neoplastic mass was performed with retrobulbar anesthesia following sedation and auriculopalpebral nerve block. On the 3rd postoperative day, 20% decreased milk yield reached normal levels. This case was found worth presenting in terms of performing a standing surgery of an OSCC located at the medial peribulbar region under sedation with retrobulbar anesthesia and regional nerve block, by applying the principles of ophthalmic surgery and inserting a penrose drain.
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