Ameneh KHOSHVAGHTI, Atbin ASKARI, Saeed NAZIFI, Mohsen GHANE

Evaluation of Some Antioxidant Enzymes in Cattle Infected with Foot and Mouth Virus

ABSTRACTThe present study was conducted to evaluate the activities of superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase and their correlation in cattle infected with foot and mouth virus (FMD). Forty cows were diagnosed and confirmed for FMD. Ten clinically healthy adult cattle were selected as control. Superoxide dismutase (SOD) glutathione peroxidase (GPx), malondialdehyde (MDA) and catalase (CAT) were measured by validated standard methods. SOD and GPx were significantly lower in the infected animals compared with the healthy group (P<0.05 in all cases). The mean concentration of malondialdehyde (MDA) was significantly higher in infected animals compared with healthy cows (P<0.05). There were no significant differences between the mean activities of catalase in control group compared with infected group. There were significant associations between (GPx) and catalase (CAT) in the control group. In the FMD group, significant associations were observed for MDA with catalase. The results of this study revealed that SOD, GPx decreased and MDA increased in FMD-infected cattle in response to viral infection. However, in the future more detailed future studies are required to characterize such responses and to improve the development of novel control strategies against FMD.Key Words: Superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase, Foot and mouth disease ÖZETŞAP VİRÜSÜ İLE İNFEKTE SIĞIRLARDA BAZI ANTİOKSİDAN ENZİMLERİN DEĞERLENDİRİLMESİBu çalışma şap virüsü (FMD) ile infekte sığırlarda süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz aktivitelerini ve bunların bağıntılarını değerlendirmek için yapılmıştır. Kırk adet ineğin FMD yönünden tanısı ve konfirmasyonu yapıldı. Kontol grubu olarak on tane klinik olarak sağlıklı yetişkin inek belirlenmiştir. Süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), malondialdehit (MDA) ve katalaz (CAT) düzeyleri geçerli standart yöntemler ile ölçüldü. İnfekte hayvanlarda SOD ve GPx değerleri kontrol grubu ile karşılaştırıldığında (P<0,05 her vakada) anlamlı olarak düşük bulundu. İnfekte hayvanlarda malondialdehit (MDA)'in ortalama konsantrasyonu kontrol grubu ile karşılaştırıldığında (P<0,05) anlamlı olarak daha yüksek bulundu. İnfekte grubu ile kontrol grubu karşılaştırıldığında katalaz düzeylerinin ortalama değerleri arasında anlamlı bir değişiklik yoktu. Kontrol grubu içinde GPx ve katalaz (CAT) değerleri arasında anlamlı bir ilişki saptandı. İnfekte grubunda katalaz ile MDA değerleri arasında anlamlı bir ilişki gözlemlendi. Bu çalışmanın sonucunda, şap virüsü ile infekte sığırlarda viral infeksiyona yanıt olarak SOD ve GPx değerlerinin azaldığı ve MDA değerlerinin arttığı belirlenmiştir. Ancak, gelecekte daha detaylı çalışmalar sayesinde bu gibi antioksidan enzimleri değişimlerinin karakterize edilmesi ve şap hastalığına karşı yeni kontrol stratejilerinin geliştirilmesi mümkün olacaktır.Anahtar Kelimeler: Süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz, Şap hastalığı

Anahtar Kelimeler:

Süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz, Şap hastalığı

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The present study was conducted to evaluate the activities of superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase and their correlation in cattle infected with foot and mouth virus (FMD). Forty cows were diagnosed and confirmed for FMD. Ten clinically healthy adult cattle were selected as control. Superoxide dismutase (SOD) glutathione peroxidase (GPx), malondialdehyde (MDA) and catalase (CAT) were measured by validated standard methods. SOD and GPx were significantly lower in the infected animals compared with the healthy group (P<0.05 in all cases). The mean concentration of malondialdehyde (MDA) was significantly higher in infected animals compared with healthy cows (P<0.05). There were no significant differences between the mean activities of catalase in control group compared with infected group. There were significant associations between (GPx) and catalase (CAT) in the control group. In the FMD group, significant associations were observed for MDA with catalase. The results of this study revealed that SOD, GPx decreased and MDA increased in FMD-infected cattle in response to viral infection. However, in the future more detailed future studies are required to characterize such responses and to improve the development of novel control strategies against FMD.

Keywords:

Superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase, Foot and mouth disease,

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Aebi, H.E., 1983. Catalase. In: Bergmeyer, H.U., (Ed.). Methods of Enzymatic Analysis. 3 rd Ed. Verlag Chemie, Weinheim 273-286.
Beck, M.A., 2001. Antioxidants and viral infections: host immune response and viral pathogenicity. Journal of the American College of Nutrition 20 (5 Suppl), 384-388.
Belsham, G.J., 1993. Distinctive features of foot-andmouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. Progress in Biophysics and Molecular Biology 60, 241-260.
Blot, W.J., Li, J.Y., Taylor, P.R., 1993. Nutrition intervention trails in Linxian, China, Supplementation with specific vitamin mineral combination cancer incidence and disease specific mortality in the general population. Journal of the National Cancer Institute 85, 1483-1492.
Clark, L.C., Combs, G.F., Turnbull, B.W., 1996. Nutritional prevention of cancer study group. Effect of selenium supplementation for cancer prevention in patients with carcinoma of skin: A randomized controlled trial. The Journal of the American Medical Association 276, 1957-1963.
Combs, G.F., Gray, W.P., 1998. Chemopreventive agents: Selenium. Pharmacology and Therapeutics 79, 179-192.
Duffield-Lillico, A.J., Reid, M.E., Turnbull, B.W., Combs, G.F., Slate, E.H., Fischbach, L.A., Marshall, J.R., Clark, L.C., 2002. Baseline characteristics and the effect of selenium supplementation on cancer incidence in a randomized clinical trial: a summary report of the nutritional prevention of cancer trial. Cancer Epidemiology, Biomarkers & Prevention 11, 630-6
Gonzales, R., Auclair, C., Voisin, E., Gautero, H., Dhermy, D., Boivin, P., 1984. Superoxide dismutase, catalase and glutathione peroxydase in red blood cells from patients with malignant diseases. Cancer Research 44, 4137- 4139.
Hagar, H.H., El-Etter, E., Arafa, M., 2006. Taurine attenuates hypertension and renal function induced by cyclosporine in rats. Clinical and Experimental Pharmacology and Physiology 33, 189-1
Halliwell, B., Gutteridge, J.M.C., 1999. Antioxidant defenses. In: Halliwell B, Gutteridge JMC, editors. Free Radicals in Biology and Medicine. 3rd ed. Oxford: Clarendon Press, pp. 200-216.
Heinonen, O.P., Albanes, D., Vitamino, J., 1998. Prostate cancer and supplementation with alpha-tocopherol and beta -carotene: incidence and mortality in a controlled trial. Journal of the National Cancer Institute 90, 440-446.
Holben, D.H., Smith, A.M., 1999. The diverse role of selenium within selenoproteins: A review. Journal of American Dietetic Association 99, 836-8
Jain, N.C., 1986. Schalms Veterinary Hematology. 4 th ed. Lea and Febiger Publication. pp. 381-383.
Kargin, F., Fidanci, U.R., 2001. Kidney diseases and antioxidative metabolism in dogs. Turkish Journal of Veterinary and Animal Sciences 25, 607-6
Kidd, P.M., 1997. Glutathione: systemic protectant against oxidative and free radical damage. Alternative Medicine Review 2 (3), 155-179.
Kotos, H.A., Wel, E.P., 1986. Superoxide production in experimental brain injury. Journal of Neurosurgery 64, 803-807.
Lopaczyski, W., Zeisel, S.H., 2001. Antioxidants, programmed cell death and cancer. Nutrition Research 21, 295-307. Lykkesfeldt, J., 200 Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophoto-metry. Clinical Chemistry 47, 1725-1727.
Patterson, R.A., Leacke, D.S., 1998. Human serum, cysteine and histidine inhibit the oxidation of low density lipoprotein less at acidic pH. Federal European Biochemistry Society Letter 434, 317-321.
Rweyemamu, M.M., Booth, J.C., Head, M., Pay, T.W.F., 1978. Microneutralization tests for serological typing and subtyping of foot and mouth disease virus strains. Journal of Hygiene 81, 107-123.
Salonen, J.T., Alfthan, G., Huttunen, J.K., Pikkarainen, J., Puske, P.P., 19 Association between cardiovascular death and myocardial infarction and serum selenium in a matched-pair longitudinal study. Lancet 2, 1751
Salt, J.S., 1993. The carrier state in foot and mouth disease: an immunological review. British Veterinary Journal 149, 207-223.
Salt, J.S., 1998. Persistent infection with foot-andmouth disease virus. Top Tropical Virology, 1, 77-1
Simsek, S., Yuce, A., Utuk, A.E., 2006. Determination of serum malondialdehyde levels in sheep naturally infected with Dicrocoelium dendriticum. Fırat Üniversitesi Sağlık Bilimleri Dergisi 20, 217-220.
Verhagen, H., Buijsse, B., Jansen, E., Bueno-deMesquita, B., 2006. The state of antioxidant affairs. Nutrition Today 41, 244-250.
Zal, F., Mostafavi-Pour, Z., Vessal, M., 2007. Comparison of the effects of vitamin E and/or quercetin in attenuating chronic cyclosporine AIncluded nephrotoxicity in male rats. Clinical and Experimental Pharmacology and Physiology 34, 720-724.