Celalettin SEVER, Fatih UYGUR, Yalçın KÜLAHÇI, Gamze KÖSE TORUN, Muammer URHAN, Zafer KÜÇÜKODACI, Günalp UZUN, Osman İPÇİOĞLU, Tuncer ÇAYCI

Hiperbarik oksijen tedavisinin sıçanlarda kemik prefabrikasyonu üzerine etkisi

Amac: Bu deneysel calışmanın amacı hidroksiapatit (HA) seramik bloklarına mezenkimal kok hucre, vaskuler pedikul ve hiperbarik oksijen tedavisi (HBOT) uygulanarak, sıcanlarda vaskularize kemik prefabrikasyonunun gercekleştirilmesidir. Calışma planı: Calışmada 45 adet erkek Sprague-Dawley sıcan, her birinde 15 sıcan olan uc gruba ayrıldı. Tum gruplarda HA seramikleri yuzeyel inferior epigastrik arter ve ven ile pedikulize edildi. Grup 2’de HA seramiklerinin icine mezenkimal kok hucre implantasyonu yapıldı. Grup 3’te ise hem mezenkimal kok hucre hem de HBOT uygulandı. Yeni kemik oluşumu ve neovaskularizasyon varlı.ı ve derecesi radyolojik, mikroanjiografik, sintigrafik, biyokimyasal ve histomorfometrik yontemler ile de.erlendirildi. Sonuclar: Neovaskularizasyon ve yeni kemik oluşumu en fazla, mezenkimal kok hucre implantasyonu yapılan ve takiben HBOT uygulanan Grup 3’te goruldu. Cıkarımlar: HBOT, neovaskularizasyon ve yeni kemik oluşumunu artırır, bu nedenle vaskulerize kemik greftlerinin optimal ve hızlı kemik prefabrikasyonunu sa.layabilir.

Effect of hyperbaric oxygen therapy on bone prefabrication in rats

using the interconnected porous coralline hydroxyapatite ceramic by combining vascular bundle implantation, mesenchymal stem cells, and hyperbaric oxygen therapy (HBOT) administration in a rat model. Methods: Forty-five male Sprague-Dawley rats were divided into three groups, each containing 15 rats. The hydroxyapatite ceramics were vascularized by the superficial inferior epigastric artery and vein in all groups. These vessels passed through the hole of the hydroxyapatite blocks. In Group 2, mesenchymal stem cells were administered into the hydroxyapatite. In Group 3, both mesenchymal stem cells and HBOT were administered. The presence and density of any new bone formation and neovascularization were evaluated by radiography, microangiography, scintigraphy, biochemical analysis, and histomorphometry. Results: Neovascularization and bone formation were significantly greater in Group 3, in which both mesenchymal stem cells and HBOT were applied, than the other groups. Conclusion: HBOT enhances neovascularization and osteogenesis, thus HBOT can provide optimal and faster prefabrication of a vascularized bone graft.

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