Ezgi AKAR, Selin TURAL EMON, Mustafa EFENDİOĞLU, Baris ERDOĞAN, Taner ENGİN, fügen aker

Effects of tamoxifen therapy on sciatic nerve crush injury: An experimental study in rats

Objective: This study aimed to determine the effects of tamoxifen on sciatic nerve crush injury in a rat model using histopathological,histomorphometric, and immunohistochemical approaches.Methods: In this study, 24 male Sprague-Dawley rats aged of 5 to 7 weeks and weighing between 300 g and 400g were used. The ratswere randomly divided into 3 groups: control (group C), sciatic nerve injury (group SNI), and sciatic nerve injury with tamoxifen (groupSNT). The sciatic nerve crush injury model was performed using the De Koning’s crush force method. In group C, only a skin incisionwas made and then the skin was sutured. In group SNI, the injury model was performed but no treatment was applied. In group SNT, theinjury model was executed, and then 40 mg/kg/day tamoxifen was given for 4 weeks by intraperitoneal methods. At the end of 4 weeks,all animals were killed using high doses of an anesthetic. Approximately, 2-cm sciatic nerve samples were obtained for histopathological,histomorphometric, and immunohistochemical analyses using the old skin incision.Results: In the histopathological examination, vascular congestion and density of vacuolization were significantly lower in group SNTthan in group SNI (p

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