Soya Bitkisindeki Glutatyon Redüktaz Aktivitesi ve mRNA Seviyesinin Kuraklık Stresinde Salisilik Asit ile Değişimleri
Bu çalışma, kuraklık stresi ve salisilik asitin (SA) soya bitkisindeki etkilerini değerlendirmek için gerçekleştirilmiştir. Bu amaçla, su stresine maruz bırakılan soya bitkisindeki SA, reaktif oksijen türleri (ROS) ve glutatyon redüktaz (GR) enzimi arasındaki ilişki incelenmiştir. Soya fasulyesi (Glycine max L. cv.) bitkileri serada kum tepsilere ekilerek büyütülmüştür. İkinci yaprak tamamen çıktığında bitkilerin yarısı bir hafta süreyle kuraklığa maruz bırakılmıştır. Bir haftanın sonunda hem kontrol hemde kuraklık stres grupları SA (200 μmol/L) ile muamele edilmiş ve iki gün sonra tüm gruplardan kesimler alınmıştır. Kontrol, kontrol+SA, kuraklık, kuraklık+SA muameleli yapraklarda glutatyon redüktaz aktivitesi ve mRNA seviyeleri ölçülmüştür. Bu çalışmada, 200 μmol/L SA muamelesi kontrol şartlarında GR aktivitesini önemli ölçüde artırmıştır. Kuraklık muameleli yapraklarda, kontroller ile karşılaştırıldığında, GR enzim aktivitesinin arttığı gözlenmiştir. Ancak su stresi altında SA muamelesi ile GR enzim aktivitesi önemli ölçüde azalmıştır. GR enzimini kodlayan genlerin transkripsiyon seviyeleri real-time PCR (Polimeraz zincirleme tepkimesi) kullanılarak ölçülmüştür. SA muamelesi kuraklığa maruz kalan soya yapraklarının GR-RNA seviyelerini hızlı bir şekilde azaltmıştır.
Anahtar Kelimeler:
Glutatyon redüktaz, kuraklık, salisilik asit, soya
Glutathione Reductase Activity in Soybean Plants and Changes in mRNA Levels with Salicylic Acid in Drought Stress
This study was carried to evaluate the effect of drought stress and salicylic acid (SA) treatments in soybean plants. Soybean (Glycinemax L. cv.) plants were grown to sown in trays of sand in greenhouse. When the second leaf was fully expanded, half of the plants were exposed to drought stress for one week. At the end of one week, half of the plants in the both control and drought stress groups were treated with SA (200 μmol/L) and two days later was taken cuttings from the whole. The activities of glutathione reductase (GR) and levels of mRNA have been measured in control, control-SA treatment, drought treament and drought-SA treatment leaves. In this study, 200 μmol/L SA treatment significantly has been increased GR activity in control conditions. Drought treated leaves have been observed elevated in the activities of the GR enzyme, compared to controls. But, under medium water deficit, GR activity significantly reducing with SA treatment. The transcript levels of the genes encoding GR enzyme have been measured using quantitative real-time PCR (Polymerase Chain Reaction). SA treatment has decreased rapidly GR -RNA levels of soybean leaves exposed to drought stress.
Keywords:
Glutathione reductase, drought, salicylic acid, soybean,
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- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 2022
- Yayıncı: Akademik Perspektif Derneği