Viral Hemorajik Septisemi Virüs Genotip le Suşlarının Çipura (Spams Aurata) ve Levrek (Dicentrarchus Labrax) Balıkları Uzerinde Patojenitelerinin Belirlenmesi

Bu çalışmada, Türkiye'de kültüıü yapılan çipura ve levrek balıklarının ülkemizden izole edilen, viral hemorajik septisemi virüs genotip 1e suşlarına karşı duyarlılıklarının belirlenmesi amaçlandı. Bu amaçla TB13/H15 ve SWl3/G izolatları ile 360'ar adet çipura (Sparus aurata) ve Levrek (Dicentrarchus labrax) üzerinde enfeksiyon denemesi gerçekleştirildi. Enfeksiyon dozu intraperitoneal 10'5 DKID50 /balık ve daldırma 10'5 DKID50 mll olarak uygulandı. Yapılan enfeksiyon denemesinin sonunda çipuralarda en yüksek mortalite oranı 13 ,3 bulunurken levrek balıklarında olarak bulundu. Ölen ve hayatta kalan balıklardan hücre kültüründe yapılan ekimler sonunda, daldırma yoluyla virüs verilen balıkların hiçbirisinden yeniden virüs izolasyonu yapılamadı. Sonuç olarak yapılan çalışma ile viral hemorajik septisemi virüsün çipura ve levrek balıklarında düşük patoj eniteye sahip olduğu ortaya konuldu.

Determination of Susceptibility of Sea Bass (Dicentrarchııs labrax) and Sea Bream (Spams aurata) to Viral Hemorrhagic Septicemia Virus Genotype le Strains

In this study, we have aimed the determination of susceptibility of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) which they have been cultured in Turkey to viral haemorrhagic septicemia Virus. For this purpose, an infection trial has been conducted on number of 360 sea bass and sea bream With using TB13/H15 and SW13/G isolates. The dose of infection applied as 10"5 TCID50 ml‘1 for infection via immersion and 10'5 TCID50 /fish for intraperitoneal infection. As result of the infection trial, the average mortality rate for sea bream and sea bass has been found as 13,3 and in the intraperitoneal infections. At the end ofthe study, virus was not isolated from dead or alive fısh which infected by immersion. In conclusion, it has been founded that viral haemorrhagic septicemia virus (Genotype le strains) has low pathogenicity on the sea bass and sea bream.

Kaynakça

Castric, J. ve de Kinkelin, P. 1984. Experimental study of the susceptibility of two marine fish species, sea bass (Dicentrarchus labrax) and turbot (Scophthalmus maximus), to viral haemorrhagic septicaemia. Aquaculture, 41 203-212.

Hedrick, R.P., Batts, W. N., Yun, S., Traxler, G.S., Kaufman, J. ve Winton, JR. 2003. Host and geographic range extensions of the North American strain of viral hemorrhagic septicemia virus. Diseases of Aquatic Organisms, 55: 211- 220.

Isshiki, T., Nagano, T. ve Miyazaki, T. 2003. Susceptibility of various marine fish species to viral hemorrhagic septicemia virus isolated from Japanese flounder. Fish Pathology, 38: 113—1 15

Ito, T., Mori, K. I., Arimoto, M. ve Nakajima, K. 2004. Virulence of viral hemorrhagic septicemia virus (VHSV) isolates from Japanese flounder Paralichthys olivaceus in rainbow trout and several species of marine fish. Fish Pathology, 39: 103-104.

Kalayci, G., Incoglu, S. ve Ozkan, B. 2006. First isolation of Viral haemorrhagic septicaemia (VHS) Virüs from turbot (Scophthalmus maximus) cultured in the Trabzon coastal area of the Black Sea in Turkey. Bulletin of the European Association ofFish Pathologists, 26: 157—161.

Mortensen, H. F., Heuer, O. E., Lorenzen, N., Otte, L. ve Olesen, N.J. 1999. lsolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Research, 63: 95—106.

Nishizawa, T., Savaş, H., Isıdan, H., Üstündağ, C., Iwamoto, H. ve Yoshimizu, M. 2006. Genotyping and Pathogenicity of Viral Hemorrhagic Septicemia Virus from Free-Living Turbot (Psetta maxima) in Turkish Coastal Area of the Black Sea. Applied and Environmental Microbiology, 72: 2373-2378.

Schlotfeldt, H. ., Ahne, W., Vestergârd—Jorgensen, P. E. ve Glende, W. 1991. Occurrence of viral haemorrhagic septicaemia in turbot (Scop— hthalmus maximus): natural outbreak. Bulletin of the European Association of Fish Pathologists, 11: 105-107.

Skall, H. F., Olesen, N. J. ve Mellergaard, S. 2005a. Prevalence of viral haemorrhagic septicaemia virus in Danish marine fishes and its occurrence in new host species. Diseases ofAquatic Organisms, 66: 145—151.

Skall, H. F., Olesen, N. J. ve Mellergaard, S. 2005b. Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming-a review. Journal ofFish Diseases, 28, 509—529.

Snow, M., Bain, N., Black, J., Taupin, V., Cunningham, CO., King, J. A., Skall, H. F. ve Raynard, R. S. 2004. Genetic population structure ofmarine viral haemorrhagic septicaemia Virus (VHSV). Diseases oquuatic Organisms, 61: 11-21.

Takano, R., Nishizawa, T., Arimoto, M. ve Muroga, K. 2000. lsolation of viral haemorrhagic septicaemia virus (VHSV) from Wild Japanese flounder, Paralichthys olivaceus. Bulletin of the European Association of ish Pathologists, 20: 186-192.

Winton, J. R., Batts, W. N., Nishizawa, T. ve Stehr, C. M. 1989. Characterization of the first North American isolates of viral hemorrhagic septi— cemia virus. Newsletter of American Fisheries Society, Fish Health Section, 17: 23.

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