Gökkuşağı Alabahk (Oncorhynchus mykiss W., 1792)Spermasında Motilite, Canlılık ve Fertilizasyon Üzerine Bir Sulandırıcı Olarak Üzüm Pekmezinin Etkisi

Bu çalışmada, gökkuşağı alabalıklarının (Oncorhynchus mykiss) dondurulan spermasına 5, ve 10 oranında katılan pekmezin spermatozoa motilitesi, canlı spermatozoa oranı ve döllenme oranı üzerine etkisi incelendi. Altı adet erkek balık sperması dimetil sülfoksit ile birlikte 5, ve 10 oranında pekmez içeren sulandırıcı ile sulandırıldı. Spermalar 0.25 ml payetlerde, sıvı azot buharında dondurularak hafta boyunca sıvı azot içerisinde muhafaza edildi. Spermatozoa motilitesi üzerine pekmez etkisi açısından, %10 oranında üzüm pekmezinin (P0.05) döllenme oranı üzerine pelanezin tüm oranlarında önemli bir fark bulunamadığı saptandı. Ancak, 10 oranında kullamlan pekmez ile dondurulan sperınalardan elde edilen döllenme oranı glikoz sulandırıcısı için elde edilen en yüksek değerden daha iyi oldu (96.4%). Çalışma sonuçlarına göre, 10 oranında pekmezin gökkuşağı alabalıklarının spermalarınm dondurulmasında kullanılabileceği kanaatine varıldı.

The Effect of Grape Molasses as an Extender on Motility, Viability and Fertility in Rainbow Trout (Oncorhynchus mykiss W., 1792) Sperm

In this study, the effects ofthree different dosages ofgrape molasses (5, 7, 10 %) as diluent were investigated on post-thaw motility, sperm viability and fertilization rate of frozen spermatozoa from the rainbow trout (Oncorhynchus mykiss). Semen from six male fish sperm was diluted with in extenders containing 7, and 10% grape molasses with dimethyl sulfoxide 7%. The semen was frozen in 0.25 ml straws in liquid nitrogen vapor and stored in liquid nitrogen for weeks. From the point of grape molasses effect over sperm motility, grape molasses level of 10 was found more beneficial (P0.05). But, the 10% grape molasses solution was better (96.4%) than the glucose solution which has the highest fertilization rate (P

Kaynakça

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Kaynak Göster

Yunus Araştırma Bülteni
  • ISSN: 1303-4456
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2001

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