Cytotoxic and Cytogenetic Effects of Bisphenol—A in Mytilus galloprovincialis (Lamarck 1819)

Bisfenol-A (BPA), çeşitli uygulamalar için sentezlenen önemli endüstriyel kimyasallardan biridir. En son çalışmalara göre sanayinin birçok alanında yaygın kullanımı nedeniyle BPA'mn çevresel konsantrasyonları yüksek risk düzeyinde bulunmaktadır. BPA'nm Mytilus galloprovincialis (Lamarck 1819) üzerine genotoksisitesinin belirlendiği çalışma bulunmamaktadır. Mikronukleus denemeleri, interfaz hücrelerin sitoplazmasındaki DNA parçacıklarının değişimine neden olan kimyasalların ve kirliliğin belirlenmesinde kullanılan mutajenite test sistemidir. Bu amaçla, micronukleus (MN) ve diğer çekirdek anormallikleri (nuklear bud, binukleus, apoptatcik çekirdek) incelenmiştir. Midyeler BPA' n1n(l2.5-100 ug-BPA/L) artan konsantrasyonları semi-statik labaratuvar şartlarında maruz bırakılmışlardır. Çalışmanm sonuçlarına göre; BPA'mn tüm konsantrasyonlan genotoxic hasarı arttırmakta ve %o 11, 33 to %o 37, 35 MN oluşumun aneden olduğu gözlenmiştir. Bu nedenle, MN testiile BPA'nm DNA düzeyinde, M. galloprovincialis' in hücreleri etkileyip etkilemediğini belirlenmeye çalışılmıştır.

Bisfenol-A'nın Mytilusgalloprovincialis (Lamarck 1819) Üzerine Sitotoksik ve Genotoksik Etkileri

Bisphenol-A (BPA), is one of the most important industrial chemicals synthesized for diverse applications. The environmental concentrations ofBPA are at high risk level due to use widely in many fields of industry according to the latest studies. However, less knowledge has been founded to the toxicity of BPA. No studies have been founded genotoxicity of BPA to the Mytilus galloprovincialis (Lamarck 1819). The Micronucleus test is biomarker of mutagenicity/genotoxicty used for determining changes in DNA fragments exposure to pollution and chemicals. For this purpose, frequency of MN and other nuclear abnormalities (binucleated, nuclear buds, fragmented/apoptotic cells) was analyzed. Increasing concentrations ofBPA (12.5- 100 ug-BPA/L) were tested for determination ofMN under semistatic conditions in the laboratory. According to the results of the study, it is revealed that all concentration of BPA increases the genotoxic damage, and elevates the micronucleus frequency from %o 33 to %o 37, 35 Thus, it was tried to determine whether BPA affected the cells of galloprovincialis (Lamarck 1819) at the level of DNA through to the MN assay.

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Kaynak Göster

Yunus Araştırma Bülteni
  • ISSN: 1303-4456
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2001

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