Nisin ve monensin’in rumen bakterilerinin saf kültürleri üzerine karşılaştırmalı etkileri

Nisin, Lactococcus lactis tarafından üretilen düşük moleküler ağırlıklı bir bakteriyosindir. Nisin’in rumen bakterilerinin saf kültürleri üzerine etkileri hakkında oldukça sınırlı düzeyde literatür bilgisi bulunmaktadır. Bu çalışmanın amacı, nisin ve monensin’in bazı Gram-pozitif ve Gram-negatif rumen bakterilerinin saf kültürleri üzerine karşılaştırmalı etkilerini araştırmaktır. Nisin ve monensin’in antibakteriyal aktivite analizleri, anaerobik koşullarda mikrodilüsyon yöntemi kullanılarak yapılmıştır. Monensin’in Gram-pozitif rumen bakterileri üzerine antibakteriyal etkisi nisin’den daha yüksek bulunmuştur. Ruminococcus albus ve Eubacterium ruminantium monensin’e karşı en duyarlı bakteriler olarak belirlenmiştir. Bu bakterilerin büyümesi, monensin tarafından sırasıyla 6 ve 12 µg/mL konsantrasyonlarında tamamen baskılanmıştır. Nisin, monensin’den farklı olarak R. albus, E. ruminantium ve Streptecoccus bovis üzerine uyarıcı etkiler göstermiştir (p

Comparative effects of nisin and monensin on pure cultures of rumen bacteria

Nisin is a low molecular weight bacteriocin which is produced by Lactococcus lactis. Literature about the effects ofnisin on pure cultures of rumen bacteria is scarce. The objective of the present study was to investigate the comparativeeffects of nisin and monensin on pure cultures of some Gram-positive and Gram-negative rumen bacteria. Theantibacterial activity assays of nisin and monensin were carried out using broth microdilution method in anaerobicconditions. Antibacterial effect of monensin on Gram-positive rumen bacteria was higher than nisin. Ruminococcusalbus and Eubacterium ruminantium were the most sensitive bacteria to monensin. Growth of these bacteria wasinhibited completely by monensin, at 6 and 12 µg/mL concentrations respectively. Nisin exhibited stimulatory effectson R. albus, E. ruminantium and, Streptecoccus bovis (p

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