Seda IŞIKLAR, Cihan ÇAKIR, Işıl KASAPOĞLU, Göktan KUSPİNAR, Kiper ASLAN, Gürkan UNCU, Berrin AVCI

Kısa Abstinens Süresiyle Ardışık Ejakülasyonun Sperm Kromatin Bütünlüğü ve Antioksidan Aktiviteye Etkisi

Üremeye yardımcı tedavi uygulamalarında tercih edilen tedavi yaklaşımına göre semen parametrelerinin embriyoloji laboratuvarı sonuçlarına ve klinik başarıya etkisi değişmektedir. Semen parametreleri abstinens süresine ve androloji laboratuvarında uygulanan yıkama protokollerine göre değişmekte ve insemine edilecek sperm materyalinin kalitesini etkilemektedir. Bu çalışmada normozoospermik erkeklerde kısa abstinens süresinin rutin semen parametrelerine, sperm kromatin ve DNA bütünlüğüne, oksidatif strese karşı gelişen antioksidan kapasiteye etkisinin değerlendirilmesi amaçlandı. Aynı hastadan ardışık ejakülasyonla 2-5 günlük abstinens süresi sonrası (n=36) ve 1 saat abstinens süresi sonrası (n=36) alınan numuneler yıkama öncesi ve yıkama sonrası değerlendirildi. Yıkama öncesinde sperm volümünün ve total motil sperm sayısının kısa abstinens grubunda anlamlı olarak azaldığı bulundu. Yıkama sonrasında gruplar arasında motilitenin değişmediği, konsantrasyonun kısa abstinens grubunda anlamlı olarak azaldığı görüldü. Abstinens süresi kısa tutulduğunda sperm kromatin hasarının ve DNA fragmantasyon oranının azaldığı, antioksidan kapasitede bir değişiklik oluşturmadığı saptandı. Sonuç olarak normozoospermik olgularda, abstinens süresinin kısa tutulması sperm konsantrasyonunu ve total progressif motil sperm sayısını azaltmakla birlikte, uygulanacak üremeye yardımcı tedavi yaklaşımına göre inseminasyonda kromatin ve DNA bütünlüğü açısından daha kaliteli sperm kullanılmasına imkan sağlayacaktır. Ardışık ejakülasyon ve abstinens süresindeki kısalma aktioksidan kapasitede olumlu ya da olumsuz bir etki oluşturmamaktadır.

Anahtar Kelimeler:

Semen analizi, sperm DNA fragmantasyonu, sperm morfolojisi, anilin mavisi, total antioksidan kapasitesi

Effect of Sequential Ejaculation with Short Abstinence Time on Sperm Chromatin Integrity and Antioxidant Activity

The effects of semen parameters on embryology laboratory results and clinical success vary according to the preferred treatment approach in assisted reproductive technology. Semen parameters vary according to the duration of abstinence and the washing protocols applied in the andrology laboratory and effect the quality of the sperm material to be inseminated. This study aimed to evaluate the effect of a short abstinence period on routine semen parameters, sperm chromatin, DNA integrity, and antioxidant capacity against oxidative stress in normozoospermic men. Samples taken from the same patient after 2-5 days of abstinence with consecutive ejaculation (n=36) and after 1 hour of abstinence (n=36) were evaluated before and after washing. It was found that sperm volume and total motile sperm count were significantly decreased in the short abstinence group before washing. It was observed that motility did not change between the groups after washing, and the concentration decreased significantly in the short abstinence group. It was determined that sperm chromatin damage and DNA fragmentation rate decreased when the abstinence period was kept short, and there was no change in antioxidant capacity. As a result, in normozoospermic cases, keeping the abstinence period short will decrease sperm concentration and total progressive motile sperm count and allow the use of higher quality sperm in terms of chromatin and DNA integrity in insemination compared to the assisted approach reproductive treatment. Sequential ejaculation and shortening of the abstinence time do not have a positive or negative effect on the antioxidant capacity.

Keywords:

Semen analysis, sperm DNA fragmentation, sperm morphology, aniline blue, total antioxidant capacity,

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